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Unrooted tree showing the phylogenetic position of B. paucivorans CF62 T within the genus Brevibacterium and related taxa. Bar, 10 nucleotide substitutions per 100 nucleotides.
Source publication
Seven isolates from various human body sites displayed general chemotaxonomic and phenotypic characteristics of the genus Brevibacterium. This was corroborated by the 16S rRNA gene sequence analysis of strain CF62T, showing a sequence similarity of 99% to Brevibacterium mcbrellneri. However, DNA-DNA hybridization, a peculiar amino acid content of t...
Context in source publication
Context 1
... Journal of Systematic and Evolutionary Microbiology 51 There is genetic, chemotaxonomic and phenotypic evidence that strains CF62 T , CF41, CF45, CF67 and CF69 belong to a single species in the genus Brevi- bacterium, distinct from the other species described in this genus and related most closely to B. mcbrellneri (Fig. 1). The name Brevibacterium paucivorans sp. nov. is proposed for this new species. Although strains CF73 and CF89 have not been subjected to genetic analysis, their chemotaxonomic and phenotypic properties suggest strongly that they belong to the new ...
Similar publications
16S rRNA gene sequence analysis showed that the type strain Brevibacterium frigoritolerans DSM 8801T had the highest similarity (99.7%) with the reference strain Bacillus simplex NBRC 15720T, followed by Bacillus muralis DSM 16288T (99.6%), Bacillus butanolivorans DSM 18926T (99.5%), and Bacillus loiseleuriae FJAT-27997T (97.9%). This relationship...
Citations
... Genetically, the sequenced ge-nomes have relatively high G + C contents within a range of 55.8-72.4 mol% (Wauters et al., 2001;Deng et al., 2020). Phenotypically, almost all of the members are nonmotile and can tolerate 10% (w/v) or even higher of NaCl, with a few exceptions. ...
Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20′54″N, 106°49′20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09′10″N, 102°04′39″E, October 2013) of South China, respectively. Optimal growth is obtained at 25–28°C (range, 4–32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0–10.0) in the presence of 0.5–1.0% NaCl (w/v) (range, 0–15% NaCl [w/v]). The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7–46.8%; ANI, 91.9–92.1%). Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively. The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T. Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001. Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene. Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp. nov. is proposed (type strain HY170T = CGMCC 1.18600T = JCM 34230T).
... bacterio.net/brevibacterium.html) (Choi et al. 2018). Brevibacterium has been isolated from various environments such as food (e.g., fermented seafood [Choi et al. 2013] and cheese [Leclercq-Perlat et al. 2000]), humans (e.g., clinical specimens [Wauters et al. 2001] and the human body [Wauters et al. 2003]), and saline environments (e.g., saline soil [Tang et al. 2008], beach sediment [Lee 2006], marine environments [Lee 2008], salt-lake sediment [Guan et al. 2010]), and other environment such as soil of ginseng field (Jung et al. 2018). ...
Purpose
To analyze the complete genome sequence of the Brevibacterium frigoritolerans ZB201705, a Brevibacterium strain was isolated from the maize rhizosphere in drought- and salt-stressed soil, and the activity of the strain under simulated drought and high salt conditions was assessed.
Methods
We used a combination of the PacBio RS and Illumina sequencing platforms to obtain the complete genome sequence of B. frigoritolerans ZB201705.
Results
The genome consists of 5,475,560 bp in a linear chromosome with no gaps, 4,391 protein-coding sequences, 39 ribosomal RNAs, and 81 transfer RNAs. The genome analysis revealed many putative gene clusters involved in defense mechanisms. In addition, an activity analysis of the strain under high-salt and simulated drought conditions helped clarify its potential tolerance to these abiotic stresses.
Conclusions
Our data revealed the complete genome sequence of the new isolated strain, and showed that it produces many proteins involved in drought and salt stress responses, suggesting that B. frigoritolerans ZB201705 may be a potential factor to increase crop yield under abiotic stresses. The information provided here on the genome of B. frigoritolerans ZB201705 provides valuable insight into rhizobacteria-mediated plant salt and drought tolerance and rhizobacteria-based solutions for agriculture under abiotic stress.
... Brevibacterium species are gram-positive, irregular, slender, rod-shaped, non-acid fast bacteria which resemble corynebacteria. At the present time, ten species are classified in this genus: B. linens, B. iodinum, B. epidermidis, B. casei, B. mcbrellneri, B. otitidis, B. avium, B. paucivorans, B. luteolum and B. sanguinis [4][5][6][7]. The main habitats of Brevibacterium sp. are dairy products, where the bacteria contribute to the aroma and color. ...
Background
Brevibacteria are obligate aerobic gram-positive rods that are associated with milk products and are also found on human skin. Brevibacterium has been reported as a rare cause of catheter related blood steam infection mainly in immunocompromised hosts such as malignancies or AIDS patients.
Case presentation
A 94-year old woman, which had a past history of diabetes mellitus and chronic heart failure, presented with high fever associated with decreased oral intake and appetite loss and was admitted to our institute. A physical examination at the time of presentation was unremarkable. On day 2, both blood cultures collected on admission became positive with coryneform organism within 24 h without Staphylococci and Brevibacterium species were identified by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Subsequently, genetic investigation by 16S ribosomal RNA analysis was performed in order to identify the organism. Finally, the result identified this pathogen as Brevibacterium paucivorans with 99.5% homology on the Ez taxon database.
The patient was started empirically on meropenem and teicoplanin for broad-spectrum antibiotic coverage. The patient’s fever finally abated and labs were also improved. On day 14, the antibiotic therapy was discontinued. The site of infections was unknown. We hereby report a case of Brevibacterium paicivorans bacteremia in an immunocompetent patient and review cases of Brevibacterium specises bacteremia previously reported. This is the first case of B. paucivorans bacteremia as far as we could search.
Conclusion
Physicians and microbiologists should be aware that Brevibacteria are uncommon but important agents which could cause opportunistic infections in immunocompetent.
... The genus shows, however, an extreme heterogeneity in physiological, biochemical, and chemical features, resulting today in the delineation of 28 Brevibacterium species which were isolated from habitats as diverse as dairy products, poultry skin, insects, soil, mural paintings, wall, clinical samples, the human microbiome, brown algae, and salt-lake, marine, or beach sediments ( > Table 7.1). Historically, B. linens and B. iodinum were the first species described within this genus (Collins et al. 1980), followed by B. casei (Collins et al. 1983), B. epidermidis (Collins et al. 1983), B. mcbrellneri (McBride et al. 1993), B. otitidis (Pascual et al. 1996), B. avium (Pascual and Collins 1999), B. paucivorans (Wauters et al. 2001), B. luteolum (Wauters et al. 2003), B. sanguinis (Wauters et al. 2004), B. celere (Ivanova et al. 2004), B. permense (Gavrish et al. 2004), B. picturae (Heyrman et al. 2004), B. samyangense (Lee 2006), B. ravenspurgense (Mages et al. 2008), B. album (Tang et al. 2008), B. marinum (Lee 2008), B. oceani (Bhadra et al. 2008), B. massiliense (Roux and Raoult 2009), B. sandarakinum (Kämpfer et al. 2010), B. salitolerans (Guan et al. 2010), B. pityocampae (Kati et al. 2010), B. siliguriense (Kumar et al. 2011), B. ammoniilyticum (Kim et al. 2012), B. daeguense (Cui et al. 2013), and B. yomogidense (Tonouchi et al. 2013). In addition to the description of novel species, other resulted from the reclassification of heterogeneous ones. ...
... Moreover, B. mcbrellneri was isolated from genital hair of patients infected with white piedra in association with Trichosporon beigelii (McBride et al. 1993). B. luteolum (Wauters et al. 2003), B. paucivorans (Wauters et al. 2001), and B. sanguinis (Wauters et al. 2004) were also associated to human infections. Isolates belonging to the Brevibacterium genus have been also implicated in corneal ulcers (Ghosheh et al. 2007) but also bacteremia (Ulrich et al. 2006). ...
Brevibacterium constitutes a ubiquitous range of species for which only the halotolerant sulfur aroma production has been utilized in the dairy food industry. Recently, however, a wide range of new isolates from different environments including soil, sediment, and seawater has been assigned to this genus. Since these isolates show quite diverse metabolic properties, they pave the way to new applications such as metal processing or bioremediation. Despite its long-time industrial use, especially as inoculants in the cheese-making industry, the genomic era for this genus has just started, with the first genomes of B. aurantiacum ATCC9174 (an industrial strain), B. massiliense DSM23039 (a pathogenic strain), and, finally, Brevibacterium sp. JC43 (a human gut strain) being released recently. Other genome sequences of Brevibacteriaceae are on their way. This includes the genome sequences from several strains of the industrially used B. linens (ATCC9172 and OC2 strains) and the genital pathogen Brevibacterium mcbrellneri. Genomic data should open investigation of the valuable but not yet fully deciphered metabolic properties of species and, on the other side, allow a better understanding of the specialization of distinct species within this genus as pathogens. Genetic manipulation of members of this genus is also not yet fully developed and tools remain to be derived from the genomic information. The recently obtained but still limited genomic data should be screened carefully with special focus on genetic elements such as genomic islands and plasmids. In addition, the investigation of mechanism underlying lateral gene transfer will also give novel insights and potentially applicable genetic tools for this genus.
... Fifty bacterial isolates were isolated from dairy product, skin and blood from cancer and kidney failure dialysis patients. The isolates were identified and classified numerically using a set of 52 phenetic characteristics such as gram staining, cellular morphology, colonial morphology, pigmentation and rod-coccus cycle, the production of many enzymes, the ability to grow at different temperatures and concentration of NaCl in addition to their ability to oxidizing different CHO [2,9,10] and sensitivity to antibiotics [11]. Other tests were also carried out. ...
Fifty bacterial isolates isolated from dairy product, skin and blood from cancer and kidney failure dialysis patients were identified to twenty two species and the following genera:- Brevibacterium, Corynebacterium, Arthrobacter, Actinomyces, Exiguobacterium, Kocuria, Micrococcus, Rothia, Rhodococcus using a set of phenetic characteristics. Twelve isolates of the different species from the genera Brevibacterium, Arthrobacter, Corynebacterium, Kocuria, Rhodococcus, Rothia were selected and probed for lin gene by polymerase chain reaction. One species Kocuria rhizophila which inhibited most of the tested organisms did not have lin gene in the chromosome, while, the species Corynebacterium glucuronolyticum, Arthrobacter comminsii, Arthrobacter oxydans have the lin gene. Our results found there wide distribution of the structural gene encoding this linocin M18 within coryneform bacteria and also in the genus Kocuria.
... Fifty bacterial isolates were isolated from dairy product, skin and blood from cancer and kidney failure dialysis patients. The isolates were identified and classified numerically using a set of 52 phenetic characteristics such as gram staining, cellular morphology, colonial morphology, pigmentation and rod-coccus cycle, the production of many enzymes, the ability to grow at different temperatures and concentration of NaCl in addition to their ability to oxidizing different CHO [2,9,10] and sensitivity to antibiotics [11]. Other tests were also carried out. ...
... We performed 16S rRNA gene sequencing, as previously described and compared the sequence results using the basic local alignment search tool with the EzTaxon database.6,7 The 16S rRNA gene amplified using a polymerase chain reaction Mastermix (Fermentas, Thermo Scientific, MA, USA) with the conserved primers PKRF (5′-ATC CTG GCT CAG AGC GAA CG-3′) and PKRR (5′-CCC TAC GGC TAC CTT GTT ACG-3′) generated a nearly complete sequence of the 16S rRNA gene (∼1365 bp) (Veriti-PCR; Applied Biosystems Inc., CA, USA). ...
Although not previously known to cause human infections, Kocuria species have now emerged as human pathogens, mostly in compromised hosts with severe underlying disease. Recently, there has been an increasing incidence of different types of Kocuria infections reported, most likely due to the adoption of better identification methods. Here, we report a case of peritonitis caused by Kocuria rosea in a diabetic nephropathy patient who was on continuous ambulatory peritoneal dialysis. Sepsis and peritonitis caused by K. rosea in our case yielded two identical Kocuria isolates from the peritoneal dialysate fluid within a period of three days. The infection was subsequently resolved by antibiotic treatment and catheter removal. In addition to reporting this case, we herein review the literature concerning the emergence of Kocuria as a significant human pathogen. The majority of cases were device-related, acquired in the hospital or endogenous, and different Kocuria species appear to share a common etiology of peritonitis. The overall disease burden associated with Kocuria appears to be high, and the treatment guidelines for diseases associated with Kocuria have not yet been clearly defined.
... Fifty bacterial strains were isolated from dairy product, skin and blood from cancer and kidney failure dialysis patients. The strains were identified and classified numerically using a set of 52 phenetic characteristics such as gram staining, cellular morphology, colonial morphology, pigmentation and rod-coccus cycle, the production of many enzymes, the ability to grow at different temperatures and concentration of NaCl in addition to their ability to oxidizing different CHO [2,9,10] and sensitivity to antibiotics [11]. Other tests were also carried out. ...
Fifty bacterial strains isolated from dairy product, skin and blood from cancer and kidney failure dialysis patients were identified to 22 species and the following genera: Brevibacterium, Corynebacterium, Arthrobacter, Actinomyces, Exiguobacterium, Kocuria, Micrococcus, Rothia, Rhodococcus and classified numerically using a set of 52 phenetic characteristics, using simple matching coefficient (Ssm) and clustering method of unweighted average linkage between groups by SPSS program. They were also grouped to 7 main clusters and 29 sub-clusters in the hierarchical tree. Twelve isolates of the different species from the genera Brevibacterium, Arthrobacter, Corynebacterium, Kocuria, Rhodococcus, Rothia were selected from the taxonomic clusters and probed for lin gene by PCR. One species Kocuria rhizophila which inhibited most of the test organism did not have lin gene in the chromosome while the species Corynebacterium glucuronolyticum, Arthrobacter comminsii, Arthrobacter oxydans have the lin gene. Our results establish a wide distribution of the structural gene encoding this linocin M18 within coryneform bacteria and also in the genus Kocuria.
... We performed 16S rRNA gene sequencing, as previously described and compared the sequence results using the basic local alignment search tool with the EzTaxon database. 6,7 The 16S rRNA gene amplified using a polymerase chain reaction Mastermix (Fermentas, Thermo Scientific, MA, USA) with the conserved primers PKRF (59-ATC CTG GCT CAG AGC GAA CG-39) and PKRR (59-CCC TAC GGC TAC CTT GTT ACG-39) generated a nearly complete sequence of the 16S rRNA gene (,1365 bp) (Veriti-PCR; Applied Biosystems Inc., CA, USA). The polymerase chain reaction product was purified (Hi-Media, Mumbai, India) and sequenced using ABI technology (Eurofins, Bangalore, India). ...
Although not previously known to cause human infections, Kocuria species have now emerged as human pathogens, mostly in compromised hosts with severe underlying disease. Recently, there has been an increasing incidence of different types of Kocuria infections reported, most likely due to the adoption of better identification methods. Here, we report a case of peritonitis caused by Kocuria rosea in a diabetic nephropathy patient who was on continuous ambulatory peritoneal dialysis. Sepsis and peritonitis caused by K. rosea in our case yielded two identical Kocuria isolates from the peritoneal dialysate fluid within a period of three days. The infection was subsequently resolved by antibiotic treatment and catheter removal. In addition to reporting this case, we herein review the literature concerning the emergence of Kocuria as a significant human pathogen. The majority of cases were device-related, acquired in the hospital or endogenous, and different Kocuria species appear to share a common etiology of peritonitis. The overall disease burden associated with Kocuria appears to be high, and the treatment guidelines for diseases associated with Kocuria have not yet been clearly defined.
... Brevibacterium ـﻲ ﺒﻴﺭﻜــ ـﻭﻋﺔ ﻤﻭﺴــ ـﻰ ﻋﻠــ ـﺎﺩﺍ ﺍﻋﺘﻤــ ﺍﻟﺘــﺼﻨﻴﻔﻴﺔ Bergey's Manual of Systematic Bacteriology ﺨﻀﺭﺍﺀ ﺍﻭ ﺼﻔﺭﺍﺀ ﺘﻅﻬﺭ ﺍﻟﺘﺤﻀﻴﻥ ﻓﺘﺭﺓ ﺯﻴﺎﺩﺓ ﻭﻋﻨﺩ ﻟﻤﺎﻋﺔ ﻤﻠﺴﺎﺀ ﻤﺤﺩﺒﺔ ﺍﻜﺜﺭ، ﺒﺭﺘﻘﺎﻟﻴﺔ ﺍﻭ ﻟﻠﺩﻡ، ﻤﺤﻠﻠﺔ ﻏﻴﺭ ، Brevibacterium ﺍﻟﻠـﻭﻥ ﺍﻋﻁـﺎﺀ ﻓـﻲ ﺘﺴﺎﻫﻡ ﺤﻴﺙ ﺍﻟﺤﻠﻴﺏ ﻤﻨﺘﺠﺎﺕ ﻓﻲ ﺍﺴﺎﺴﻴﺔ ﺒﺼﻭﺭﺓ ﺘﻭﺠﺩ ﻁﺒﻴﻌـﻲ ﺒﺸﻜل ﺍﻻﻨﺴﺎﻥ ﺠﻠﺩ ﻋﻠﻰ ﺘﻭﺠﺩ ﻭﺍﻴﻀﺎ ﺍﻟﺠﺒﻥ ﻟﺴﻁﺢ ﺍﻟﻤﻤﻴﺯﺓ ﻭﺍﻟﺭﺍﺌﺤﺔ Brevibacterium ................ 14 ﺍﻟﺭﻁﺒﺔ ﺍﻟﻤﻨﺎﻁﻕ ﻓﻘﺩ ﺍﻟﺠﺴﻡ ﻤﻥ ﺍﻟﻤﻨﺜﻨﻴﺔ ﻭﺍﻟﻤﻨﺎﻁﻕ ﺍﻻﻗﺩﺍﻡ ﺒﻴﻥ ﻤﻥ ﻋﺯﻟﺕ ﻫﻲ ﻭ ﻜﺭﻴﻬﺔ ﺭﺍﺌﺤﺔ ﺍﻋﻁﺎﺀ ﻓﻲ ﺘﺴﺎﻫﻡ ﻟﻠﺠﺴﻡ ) ﺍﻫﻤﻬﺎ ﺍﻻﻨﻭﺍﻉ ﻤﻥ ﺍﻟﻌﺩﻴﺩ ﺍﻟﺠﻨﺱ ﻴﻀﻡ ﺤـﺭﺍﺭﺓ ﺩﺭﺠـﺔ ﻋﻨـﺩ ﺍﻟﻨﻤـﻭ ﺘﻔﻀل ﺍﻟﺘﻲ ﻭﺍﻟﻨﻭﻉ ﺍﻟﺩﻤﻭﻴﺔ ﺍﻟﺩﻴﻠﺯﺓ ﺴﺎﺌل ﻭﻤﻥ ﺍﻻﻨﺴﺎﻥ ﺠﻠﺩ ﻤﻥ ﻭﺍﻟﻤﻌﺯﻭﻟﺔ ﻡ ﺍﻟﺒﻴـﻀﺎﺀ ﺒﺎﻟﻌﻘﺩﺓ ﺍﻻﺼﺎﺒﺔ ﻤﻥ ﻴﻌﺎﻨﻭﻥ ﺍﻟﺫﻴﻥ ﻟﻠﻤﺭﻀﻰWinn et al., 2006 ;Funke et al., 1997 ﺍﻟﺴﺭﻴﺭﻴﺔ ﺍﻟﻌﻴﻨﺎﺕ ﻤﻥ ﺍﻟﻤﻌﺯﻭﻻﻥ(Wauters et al., 2001) ...
