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Ultraviolet-visible (UV-vis) spectroscopic analysis and the color change of silver nanoparticles (Ag-NPs) synthesized by endophytic S. laurentii R-1. (A) the UV-vis spectroscopy; (B) the AgNO3 solution; (C) the S. laurentii biomass filtrate, and (D) the biosynthesized Ag-NPs.
Source publication
Improvement of the medical textile industry has received more attention recently, especially with widespread of microbial and viral infections. Medical textiles with new properties, such as bacterial pathogens self-cleaning, have been explored with nanotechnology. In this study, an endophytic actinomycetes strain of Streptomyces laurentii R-1 was i...
Contexts in source publication
Context 1
... the current study, the actinomycetal mediated synthesis of nanoparticles was optimized by adding 1 mM pure AgNO3 solution to the washed cell-free extract, incubated for 24 h, at 35° C (pH= 9). The extracellular synthesis of nanomaterials was inferred by the color of the liquid shifting from colorless to yellowish-brown ( Figure 2B-D). Silver nitrate solution and/or biomass filtrate were tested as a negative control and did not exhibit any color change after the incubation periods. ...
Context 2
... spectroscopic analysis confirmed the biogenic synthesis of Ag-NPs, which displayed a surface plasmon resonance (SPR) with a specific sharp peak at 420 nm, which was characteristic for the spherical Ag-NPs ( Figure 2A) [31]. Fouda et al. [17] reported that the SPR range for Ag-NPs synthesized by endophytic Streptomyces spp. was 440-450 nm and that the standard SPR peak for AgNPs synthesized by the green approach was from 400 to 450 nm [32]. ...
Citations
... It is clear from our results that SeNPs and AgNPs affect the percentage of observed larval mortality, increasing gradually with the increase of concentration. The estimated LC 50 values, at 95% probability, were 39.2 and 52 ppm for larvae treated with SeNPs and AgNPs, respectively after 24 h (Table 1). ...
... The successful management of several insect pests has been done in recent years, thanks to the application of nanotechnology in all disciplines, including pesticide preparations. Due to their low toxicity, environmental friendliness, and affordability, green-synthesized AgNPs are employed more frequently than other metal NPs [50,51]. The effective 70.000 ± 0.00 functionality group of a plant chemical embedded with an Ag ion-containing liquid during the reduction stage resulted in the formation of tiny size NPs, which is the mechanism of this green synthesis [52]. ...
Some of the significant globally prevalent vector-borne illnesses are caused by Culex pipiens. Synthetic pesticides have been widely utilized to eradicate C. pipiens, which has led to a number of health risks for people, insect resistance, and environmental contamination. Alternative strategies are therefore vitally needed. In the current investigation, the Trichoderma viride fungal culture filtrate was used to create selenium and silver nanoparticles (SeNPs and AgNPs, respectively) and test them on C. pipiens larvae in their fourth instar stage. The death rate increased significantly when SeNP and AgNP concentrations increased, according to the results. SeNPs and AgNPs significantly affected the developmental and detoxification enzymes in fourth instar larvae of C. pipiens at 24 h after being treated with the sublethal concentration of the tested NPs. As a result of their insecticidal effect on C. pipiens larvae, SeNPs and AgNPs are considered effective and promising larvicidal agents.
... Chemotherapy is the most common method for treating patients with colon cancer [1,4]. Nonetheless, chemotherapy drugs not only target cancerous cells but also damage normal body tissues and cause side effects [4,5,6]. Therefore, antitumor drug carriers that maintain or improve the effectiveness of chemotherapy while reducing the severity of reactions and side effects are much needed [1]. ...
... So far, no ndings related to the toxicity of these nanoparticles have been reported [11,12]. Nanoparticles can be synthesized through various methods, including physical, chemical, and biological approaches [5]. However, the synthesis conditions in synthetic methods,such as the use of hazardous chemicals, acidic pH, and high temperatures, limit their application in biomedical. ...
... Actinomycetes are promising candidates for both extracellular and intracellular production of metal nanoparticles due to the production of strong bioactive secondary metabolites, easy transportation, the lowest cost, and the least exploration. The nanoparticles produced by Actinomycetes have good stability and with the ability of genetic manipulation, they are a very suitable option [4,5]. The aim of this study is to biosynthesize ZnO NPs utilizing the actinomycete extract of Amycolatopsis roodepoortensis strain EA7, isolated from agricultural soils in the Alborz mountain range of Iran (southwest of Guilan province). ...
The present study describes the biosynthesis of zinc oxide nanoparticles using the extract from the actinomycete isolate Amycolatopsis roodepoortensis strain EA7. The 16S rRNA gene analysis revealed that strain EA7 belonged to the Amycolatopsis roodepoortensis species with 99.63% confidence. Their physical characteristics were examined using XRD, FTIR, SEM, TEM, DLS, Zeta potational, and EDX. Microscopic examination revealed spherical ZnO NPs with an estimated average size of 2.98 ± 0.69 nm. The antibacterial and anticancer effect (colon cancer cell line HT-29) of these nanoparticles was observed in a dose-dependent manner. The values of IC50 ZnO-NPs for cell line HT-29 was calculated as 47 µg/ml. Also, the expression level of ATM, ATR, CHK1 and CHK2 genes effective in apoptosis as compared with the control sample in the cell line HT29 was increased as 2.35 ± 0.293, 2.87 ± 0/280, 3.67 ± 0.378, and 5.86 ± 0.495, respectively(P < 0.0001), which indicates the induction Apoptosis in HT-29 cells. However, there was no significant differences in reducing the expression of MMP-9 and BCL-2 genes effective in carcinogenesis as compared with the control sample. Also, flowcytometry results showed, 46.74% of the cells HT-29 entered the apoptosis stage (P < 0.0001). In the analysis of the cell cycle in the Sub-G1 phase, the percentage of cell population increased (35.60%), which indicates the induction of apoptosis (P < 0.001). Given the high antimicrobial and anticancer potential of the synthesized nanoparticles, this research represents a significant step towards further studies on these nanoparticles and the development of a new generation of antimicrobial and anticancer agents.
... Previously, the cytotoxicity of Ag NPs against various cancer cell lines has been reported in some studies. [34,[85][86][87] For example, in a recent study by Zubair et al., they synthesized silver nanoparticles using Acacia nilotica extract and tested them against HepG2 in vitro. [65] They reported that Ag NPs synthesized with the green approach showed promising results in HepG2 cells. ...
... On the other hand, the band detected at 1022.50 cm −1 could be allotted to the amines functional group [61]. The observed peak at 826.27 cm −1 might be ascribed to the bending motion of the carbon-carbon double bond (C]C) present in alkenes [62]. Furthermore, the band at 764.10 cm −1 might be ascribed to the C-Cl bond of halo compounds [63]. ...
The build-up of synthetic dyes in the environment and aquatic ecology is a significant environmental issue due to their inability to break down naturally. The overuse of chemical fungicides also poses a threat to the environment due to their accumulation and fostering of fungal resistance. Hence, the study was conducted to detect the antifungal properties and photocatalytic activity of greenly synthesized iron oxide nanoparticles (IONPs) prepared using the Hibiscus sabdariffa flower extract. The biogenic IONPs showed the highest photocatalytic activity against rhodamine B dye at a concentration of 4.0 mg/ml. The biogenic IONPs also demonstrated effective antifungal properties against Penicillium digitatum and Aspergillus niger strains, with relative inhibition percentages of mycelial growth being higher than those with the metalaxyl + mancozeb fungicide at 800 ppm concentration. The efficient photocatalytic activity of the biogenic IONPs against rhodamine B dye and their effective antifungal properties suggest their potential use as safe substitutes for commercial fungicides.
... Metal nanoparticles (M NPs) are widely studied in the fields of antibacterial catalysis [17][18][19], wastewater treatment [20], and biomedicine [21], due to their unique physicochemical properties, and are a popular research direction in materials science [22]. M NPs are usually synthesized using kettle reactors, but the synthesis process is prone to agglomeration, which leads to decreased catalytic effect, poor dispersibility and controllability [23]. ...
... The hydroxyl, carbonyl, and amine functional groups in these extracted metabolites react with metal ions to reduce their size to the nano-range. Flavonoids, in general, have various functional groups, and it is thought that the OH group of flavonoids is primarily responsible for the reduction of metal ions into NPs [12][13][14]. These molecules not only assist in the bio-reduction of ions to nanoscale sizes but also contribute to the capping of nanoparticles, which is critical for stability and biocompatibility. ...
In our study, we examined how well six Streptomyces strains bio-fabricated ZnONPs, MnONPs, and/or ZnO/MnO 2 nanocomposite. The most potent strain that generated efficient antimicrobial nanoparticles was then picked to increase the production of those particles in a semi-industrial pilot plant unit. Consequently, the intracellular extract of endophytic Streptomyces coelicolor strain E72 was used to achieve the bio-fabrication reaction of the spherical ZnO/MnO 2 nanocomposite (6–18 nm). The bio-fabricated ZnO/MnO 2 nanocomposite was validated and characterized using FTIR, XRD, SEM, TEM, TGA, and EDS analyses. Additionally, the production of this ZnO/MnO 2 nanocomponent was scaled up to a pilot plant unit with a semi-industrial size. The Plackett–Burman experimental method was used to maximize the production of ZnO/MnO 2 nanocomposites, which had increased 2.7-fold from their initial state. The bio-fabricated ZnO/MnO 2 nanocomposite was subsequently scaled up 31.25 times using an exponential pulse-feeding fermentation technique in a 70-L bioreactor. This ZnO/MnO 2 nanocomposite exhibited effective antimicrobial efficacy against all tested antibiotic-resistant human pathogens. The antimicrobial effects against Salmonella paratyphi (53.17 ± 2.8 mm) and Candida albicans (50.2 ± 1.01 mm) were the most potent at 90 and 130 µg/ml of ZnO/MnO 2 nanocomposite, respectively. This is the first full explanation of the ZnO/MnO 2 nanocomposite bio-fabrication at a semi-industrial scale employing endophytic strain E72 extract as a reducing/capping agent that reacted with MnCl 2 ·4H 2 O and Zn (CH 3 COO) 2 ·2H 2 O as precursors. This bio-fabricated ZnO/MnO 2 nanocomposite has the potential to be utilized in the development of pharmaceuticals, cosmetics, wound dressings, and burn therapy due to its powerful antimicrobial capabilities.
... Streptomyces is a filamentous Gram-positive bacterial genus, known for safety and human non-pathogenicity, producing various secondary metabolites, pollution free and environment friendlier. So among Actinomycetes, Streptomyces is the most candidates in the field of nanotechnology for the biological synthesis of Ag-and Cu-NPs on large scale and at low cost (Eid et al. 2020;Prasad et al. 2016). ...
Microbial resistance and biofilm formation have been considered as the main problems associated with microbial resistance. Several antimicrobial agents cannot penetrate biofilm layers and cannot eradicate microbial infection. Therefore, the aim of this study is the biological synthesis of silver and copper nanoparticles to assess their activities on bacterial attachment and on the viability of dormant cells within the biofilm matrix. Ag-NPs and Cu-NPs were biosynthesized using Streptomyces isolate S29. The biologically synthesized Ag-NPs and Cu-NPs exhibited brown and blue colors and were detected by UV/Vis spectrophotometry at 476 and 594 nm, respectively. The Ag-NPs showed an average size of 10–20 nm as indicated by TEM, and 25–35 nm for Cu-NPs. Both Ag-NPs and Cu-NPs were monodispersed with a polydispersity index of 0.1–0.546 and zeta potential were − 29.7, and − 33.7 mv, respectively. The biologically synthesized Ag-NPs and Cu-NPs significantly eliminated bacterial attachment and decreased the viable cells in the biofilm matrix as detected by using crystal violet and tri-phenyl tetrazolium chloride assays. Furthermore, Ag-NPs and Cu-NPs significantly eradicated mature biofilms developed by various Gram-negative pathogens, including A. baumannii, K. pneumoniae and P. aeruginosa standard strains and clinical isolates. Data were also confirmed at the molecular level with prominent elimination of biofilm gene expression carO, bssS and pelA in A. baumannii, K. pneumoniae and P. aeruginosa, respectively compared to untreated cells under the same conditions. As indicated, Ag-NPs and Cu-NPs could be used as adjuvant therapy in eradication of antibiotic resistance and biofilm matrix associated with Gram-negative bacterial infection.
Graphical Abstract
... NPs have recently been used in various industries, including agriculture, medicine, pharmaceuticals, textile industries, beauty products, biodegradation or photocatalytic processes, and archaeological preservation. Several NPs, such as MgO, CuO, ZnO, Ce 2 O, and others, are successfully synthesized by biological methods [22][23][24][25][26][27]. ...
... albicans biofilm action. Previous studies have proven that silver nanoparticles are effective against MDR pathogens and nosocomial infections [128][129][130][131]. Roberto et al. demonstrated that AgNPs can exert promising antifungal activity against MDR C. auris, whether present in planktonic form or sessile in biofilm [87]. ...
An emerging multidrug-resistant pathogenic yeast called Candida auris has a high potential to spread quickly among hospitalized patients and immunodeficient patients causing nosocomial outbreaks. It has the potential to cause pandemic outbreaks in about 45 nations with high mortality rates. Additionally, the fungus has become resistant to decontamination techniques and can survive for weeks in a hospital environment. Nanoparticles might be a good substitute to treat illnesses brought on by this newly discovered pathogen. Nanoparticles have become a trend and hot topic in recent years to combat this fatal fungus. This review gives a general insight into the epidemiology of C. auris and infection. It discusses the current conventional therapy and mechanism of resistance development. Furthermore, it focuses on nanoparticles, their different types, and up-to-date trials to evaluate the promising efficacy of nanoparticles with respect to C. auris.
... This indicates the high stability of synthesized CuO-NPs, the analysis was carried out in a wide range of ζ-values (positive and negative values) to detect all charges on the NPs surface. The ζ-potential spectrum showed that all CuO-NPs have a negative charge, which indicate that the particles remain for-away from each other, and this prevent the agglomeration or aggregation with time [76]. Also, the presence of varied capping agent that cover the surface of NPs can be increase the stability as reported previously [77]. ...
Background
The overuse of antibiotics leads to the emergence of antibiotic-resistant microbes which causes high mortality worldwide. Therefore, the synthesis of new active compounds has multifunctional activities are the main challenge. Nanotechnology provides a solution for this issue.
Method
The endophytic fungal strain Aspergillus terreus BR.1 was isolated from the healthy root of Allium sativum and identified using internal transcribed spacer (ITS) sequence analysis. The copper oxide nanoparticles (CuO-NPs) were synthesized by harnessing the metabolites of the endophytic fungal strain. The UV-Visble spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), Transmission electron micrscopy (TEM), Energy dispersive X-ray (EDX), X-ray diffraction (XRD), Dynamic light scattering (DLS), and zeta potential (ζ) were used for the characterization of synthesized CuO-NPs. The activity against different pathogenic bacteria and Candida species were investigated by agar well-diffusion method. The biocombatibility and anticancer activity were assessed by MTT assay method. The scavenging of DPPH was used to investigate the antioxidant activity of synthesized CuO-NPs.
Results
Data showed the successful formation of crystalline nature and spherical shape CuO-NPs with sizes in the ranges of 15–55 nm. The EDX reveals that the as-formed sample contains ions of C, O, Cl, and Cu with weight percentages of 18.7, 23.82, 11.31, and 46.17%, respectively. The DLS and ζ-potential showed high homogeneity and high stability of synthesized CuO-NPs with a polydispersity index (PDI) of 0.362 and ζ-value of − 26.6 mV. The synthesized CuO-NPs exhibited promising antibacterial and anti-Candida activity (concentration-dependent) with minimum inhibitory concentration (MIC) values in the ranges of 25–50 µg mL–1. Moreover, the fungal mediated-CuO-NPs targeted cancer cells of MCF7 and PC3 at low IC50 concentrations of 159.2 ± 4.5 and 116.2 ± 3.6 µg mL–1, respectively as compared to normal cells (Vero and Wi38 with IC50 value of 220.6 ± 3.7 and 229.5 ± 2.1 µg mL–1, respectively). The biosynthesized CuO-NPs showed antioxidant activity as detected by the DPPH method with scavenging percentages of 80.5 ± 1.2% at a concentration of 1000 µg mL–1 and decreased to 20.4 ± 4.2% at 1.9 µg mL–1 as compared to ascorbic acid (control) with scavenging activity of 97.3 ± 0.2 and 37.5 ± 1.3% at the same concentrations, respectively.
Conclusion
The fungal mediated-CuO-NPs exhibited promising activity and can be integrated into various biomedical and theraputic applications.
