UVC treatment and SPA of ICP1 A. UVC treatment of ICP1 resulted in a significant reduction in viable phage. Rows 1-3 are UV treated and show 0-2 infective phages in three replicates when undiluted (dilution factor noted at the top of panel). Row 4 is a negative control, a grid containing ICP1 that remained in the grid box during treatment. B. A representative micrograph of UVC-treated ICP1 showing an assembled phage (far right), an empty and full head (middle/left), and liposomes (round, bottom). C & D. SPA analysis resulted in structures of the control and UVC treated ICP1 phage with a resolutions of 7.1 Å and 6.6 Å, respectively. E. Determination of the T number = 13, which a measure of the icosahedral