Figure 1 - available from: Scientific Reports
This content is subject to copyright. Terms and conditions apply.
Source publication
Phycoerythrin is the main phycobiliprotein that responsible for harvesting light for photosynthesis in cyanobacteria. In this research, phycoerythrin extracted from the cyanobacterium Nostoc carneum has been used to reduce silver nitrate for silver nanoparticles (AgNPs). UV–visible spectrophotometry for measuring surface plasmon resonance showed a...
Contexts in source publication
Context 1
... are classified based on their spectral properties. Phycoerythrin (PE, λ A max = 540-570 nm), phycocyanin (PC, λ A max = 610-620 nm) and allophycocyanin (APC, λ A max = 650-655 nm) which are the mostly detected phycobiliproteins 13 . In the present study, phycoerythrin extracted from Nostoc carneum has λ A max = 560 nm that displayed in Fig. 1 AgNPs synthesis by phycoerythrin. Silver nanoparticles biosynthesis was performed by addition of 1 mL of 0.8 mg/mL of lyophilized phycoerythrin solution to 19 mL (20 mM) of aqueous AgNO 3 and kept in illumina- tion conditition. The pink color of phycoerythrin reaction mixture turned into dark brown color which demon- strating AgNPs ...
Context 2
... and dispersion of the synthesized AgNPs. The stability and the degree of dispersion of the synthesized AgNPs was studied by keeping AgNPs under dark condition in closed bottle for 1 day, 6 months and 9 months. It has been observed that, there is no significant change in color or appearance of agglomeration over a period of 6 months (Fig. 10A). The plasmonic properties characterized by UV-visible spectroscopy. UV-vis spectra indi- cated a shift in the peak position of the surface plasmon resonance from 430 to 432 nm with the stored AgNPs on 9 th month (Fig. 10B). Also, size and morphology of synthesized AgNPs were validated using transmission electron microscopy (TEM). TEM ...
Context 3
... It has been observed that, there is no significant change in color or appearance of agglomeration over a period of 6 months (Fig. 10A). The plasmonic properties characterized by UV-visible spectroscopy. UV-vis spectra indi- cated a shift in the peak position of the surface plasmon resonance from 430 to 432 nm with the stored AgNPs on 9 th month (Fig. 10B). Also, size and morphology of synthesized AgNPs were validated using transmission electron microscopy (TEM). TEM images presented in Fig. 5 for synthesized AgNPs after 1 day of synthesis and in Fig. 10C for stored AgNPs revealed that the AgNPs were well dispersed and there was no significant alteration in the size and the shape of ...
Context 4
... UV-vis spectra indi- cated a shift in the peak position of the surface plasmon resonance from 430 to 432 nm with the stored AgNPs on 9 th month (Fig. 10B). Also, size and morphology of synthesized AgNPs were validated using transmission electron microscopy (TEM). TEM images presented in Fig. 5 for synthesized AgNPs after 1 day of synthesis and in Fig. 10C for stored AgNPs revealed that the AgNPs were well dispersed and there was no significant alteration in the size and the shape of AgNPs and no agglomeration tendency was noticed for a period of 6 months. So we can consider that the AgNPs show better long-term stability. The dispersion stability of the AgNPs after a period of 6 months ...
Context 5
... noticed for a period of 6 months. So we can consider that the AgNPs show better long-term stability. The dispersion stability of the AgNPs after a period of 6 months was further analyzed by quantitative analysis of particle size distribution using Minitab 16 Statistical Software to calculate the mean size of particles which was found to be 15.84 (Fig. ...
Context 6
... efficiency of AgNPs. In this study, the antibacterial efficiency of AgNPs was assessed against Staphylococcus aureus and Streptococcus sp. (G + ve bacteria), in addition, Enterobacter aerogenes and E. coli (G − ve bacteria) using the method of disc-diffusion as shown in Table 4 and Fig. 11. AgNO 3 solution with 20 mM concentration was used as control. The highest antibacterial efficiency was noticed against E. coli (21 mm of inhi- bition zone) while a lower efficiency was noticed against Staphylococcus aureus (16 mm of inhibition zone). The diameter of inhibition zones against Streptococcus sp. and Enterobacter aerogenes ...
Context 7
... vitro anti-cancer activity. The cell line death rate of MCF-7 is demonstrated in Fig. 12 which increases with increase in AgNPs concentration. The IC 50 (the half maximal inhibitory concentration) value is the concentra- tion of AgNPs required to produce cell growth inhibition of MCF-7 cell line by 50% after 48 hours of incubation compared to untreated controls. The IC 50 of the biosynthesized AgNPs was recorded at 13.07 ± ...
Citations
... This occurs through a series of events including interruption of intracellular oxygen reduction, interaction with respiratory chain proteins on cell membranes, generation of free radicals and reactive oxygen species (ROS) production that leads to cellular oxidative stress. Overall, these findings provide substantial support for the antibacterial activity of SAgNPs synthesized using S. platensis extract, which is mediated by multiple mechanisms including physical disruption of the cell wall, interaction with cellular components, and induction of oxidative stress [41][42][43][44]. ...
The development of nanoparticles derived from noble metals has garnered significant attention in recent years, particularly with gold and silver being identified as prominent options for production of nanoparticles. The increasing interest in silver nanoparticles synthesis is due to its multidimensional applications such as water treatment, biosensor, food packaging and preservation, anti-microbial, anti-cancer and anti-inflammatory. Based on its diverse applications, the present study aimed to synthesize silver nanoparticles (SAgNPs) using Spirulina platensis extract as a green source and evaluate their antibacterial, antioxidant, and biocompatible properties. Techniques including UV spectroscopy, scanning electron microscopy (SEM) and Fourier transform-infrared spectroscopy (FTIR) were used to characterize the synthesized SAgNPs. The antioxidant activity was determined through DPPH radical scavenging activity, while the antibacterial activity was assessed using disk diffusion method. Results from UV- spectroscopy revealed a peak absorption at the range of 400–500 nm, with a prominent peak at 423 nm, which confirms the successful biosynthesis of SAgNPs. SEM analysis indicated an average particle size ranging from 50–70 nm. Results from FTIR analysis identified different functional groups (OH-, carbonyl and amino acids) which stabilized the nanoparticles synthesis. Hemolysis assay demonstrated less than 5% hemolysis at all the concentrations studied (100–12.5 µM) that represents the biocompatible nature of SAgNPs. The synthesized SAgNPs showed potent antibacterial activity against both S. aureus and E. coli. Furthermore, the nanoparticles displayed notable antioxidant activity, as evidenced by the antioxidant assay. In conclusion, the biosynthesized SAgNPs derived from Spirulina platensis demonstrated potential antibacterial, antioxidant, and biocompatible properties. These findings highlight the potential of utilizing Spirulina platensis for the biosynthesis of AgNPs with diverse applications in biomedical and pharmaceutical fields.
... In the economic field, its yield in the global market is projected to be more than 3 trillion USD until 2020 and more than 15 billion dollars of investment has been done in the last few years [23]. Nanomaterials have emerging significance due to their distinct physical, chemical, and electrical characteristics, while bulk materials have fixed physical properties [24]. In the present scenario, rapid increase in urbanization, industrialization, and fossil fuel consumption has badly affected the natural ecosystem so it has become very important to explore eco-friendly strategies to cope with these hazards and nanobiotechnology is one of them [25]. ...
Nanobiotechnology has gained significant attention due to its capacity to generate substantial benefits through the integration of microbial biotechnology and nanotechnology. Among microbial organisms, Actinomycetes, particularly the prominent genus Streptomycetes, have garnered attention for their prolific production of antibiotics. Streptomycetes have emerged as pivotal contributors to the discovery of a substantial number of antibiotics and play a dominant role in combating infectious diseases on a global scale. Despite the noteworthy progress achieved through the development and utilization of antibiotics to combat infectious pathogens, the prevalence of infectious diseases remains a prominent cause of mortality worldwide, particularly among the elderly and children. The emergence of antibiotic resistance among pathogens has diminished the efficacy of antibiotics in recent decades. Nevertheless, Streptomycetes continue to demonstrate their potential by producing bioactive metabolites for the synthesis of nanoparticles. Streptomycetes are instrumental in producing nanoparticles with diverse bioactive characteristics, including antiviral, antibacterial, antifungal, antioxidant, and antitumor properties. Biologically synthesized nanoparticles have exhibited a meaningful reduction in the impact of antibiotic resistance, providing resources for the development of new and effective drugs. This review succinctly outlines the significant applications of Streptomycetes as a crucial element in nanoparticle synthesis, showcasing their potential for diverse and enhanced beneficial applications.
... The present model has an R 2 value of 0.9779. A high level of correlation was considered to be achieved when the model's (R 2 ) value is more than 0.9 25 . When the R 2 value is relatively close to 1, the model is more accurate in predicting the response 102 . ...
Chitosan is a natural non-toxic, biocompatible, biodegradable, and mucoadhesive polymer. It also has a broad spectrum of applications such as agriculture, medical fields, cosmetics and food industries. In this investigation, chitosan nanoparticles were produced by an aqueous extract of Cympopogon citratus leaves as a reducing agent. According to the SEM and TEM micrographs, CNPs had a spherical shape, and size ranging from 8.08 to 12.01 nm. CNPs have a positively charged surface with a Zeta potential of + 26 mV. The crystalline feature of CNPs is determined by X-ray diffraction. There are many functional groups, including C꞊C, CH2-OH, C–O, C-S, N–H, CN, CH and OH were detected by FTIR analysis. As shown by the thermogravimetric study, CNPs have a high thermal stability. For the optimization of the green synthesis of CNPs, a Face centered central composite design (FCCCD) with 30 trials was used. The maximum yield of CNPs (13.99 mg CNPs/mL) was produced with chitosan concentration 1.5%, pH 4.5 at 40 °C, and incubation period of 30 min. The antifungal activity of CNPs was evaluated against phytopathogenic fungus; Fusarium culmorum. A 100% rate of mycelial growth inhibition was gained by the application of 20 mg CNPs/mL. The antitumor activity of the green synthesized CNPs was examined using 6 different cell lines, the viability of the cells reduced when the concentration of green synthesized CNPs increased, the IC50 dose of the green synthesized CNPs on the examined cell lines HePG-2, MCF-7, HCT-116, PC-3, Hela and WI-38 was 36.25 ± 2.3, 31.21 ± 2.2, 67.45 ± 3.5, 56.30 ± 3.3, 44.62 ± 2.6 and 74.90 ± 3.8; respectively.
... For example, Phycoerythrin from Nostoc carneum was effectively employed to produce silver nanoparticles in an environmentally friendly green technique. According to the results, biosynthesized AgNPs have a large potential for usage in cosmetic, pharmacological, medical, and therapeutic applications since they are very effective against hemolysis, germs, and cancer (El-Naggar et al. 2018). More research is needed to determine the toxicity and bioaccumulation of silver nanoparticles. ...
Marine resources are attracting a lot of interest from biologists and chemists due to their sophisticated and potent biological uses. Marine resources are diversified to usher in a new era of marine flora. Marine natural Pigments (MNPs) such as Chlorophyll, Carotenoids, and Phycobiliproteins can give therapeutic advantages. MNPs have many therapeutic applications including antioxidants, anti-inflammatory, anti-obesity, anti-angiogenic, and many more. Because of their numerous advantages over synthetic medications, MNPs can be used in the therapeutic and diagnosis of serious diseases. Discussed biological activities of MNPs strengthen the semi-synthetic pathway of drug discovery and open a new era of marine pharmaceuticals through computational study. In computational study, the molecule isolated from MNPs can have a stronger influence on both therapeutic and diagnostic outcomes. As a biomarker, MNP-derived fluorophores can be exploited in early diagnostic procedures. The primary goals of this study are to present an up-to-date view of recent research on the therapeutics and diagnostics advantages of MNPs, as well as structural properties and a list of marine organisms that generate various MNPs. We also discussed current trends and future prospects of the MNPs Market. This review encourages researchers to develop potent drugs with fresh insights into the possible function of MNPs to improve human health.
... An intensive signal at -27 mV observed with NL(C)-SeNPs can contribute to the presence of negatively charged species on the surface of the nanoparticles. [38] The zeta potential results correlate with the previous study by Zhang et al., in which the SeNPs synthesised using green tea, and Lycium barbarum polysaccharides showed a zeta potential of -24.1 mV [39] suggesting a tendency to repel each other and contribute towards colloidal stability. Furthermore, according to the previous report by Gunti et al., the negative charge on the surface of the nanoparticles can be primarily due to reducing species such as alkaloids, phenolic compounds and flavonoids [40]. ...
... An intensive signal at -27 mV observed with NL(C)-SeNPs can contribute to the presence of negatively charged species on the surface of the nanoparticles. [38] The zeta potential results correlate with the previous study by Zhang et al., in which the SeNPs synthesised using green tea, and Lycium barbarum polysaccharides showed a zeta potential of -24.1 mV [39] suggesting a tendency to repel each other and contribute towards colloidal stability. Furthermore, according to the previous report by Gunti et al., the negative charge on the surface of the nanoparticles can be primarily due to reducing species such as alkaloids, phenolic compounds and flavonoids [40]. ...
Selenium has many beneficial bioactive properties yet has a narrow therapeutic window. This problem can be addressed by selenium in nanoform or selenium nanoparticles (SeNPs). There are several chemical and physical approaches that can be employed for the synthesis of SeNPs. However, the biological route for SeNP synthesis is known to be more eco-friendly, economical, and biocompatible when assessing bioactivities. The present study demonstrates a biological approach that effectively facilitates the synthesis and stabilization of SeNPs with the help of secondary metabolites derived from endophytic fungi N. guilinensis i.e., NL(C)-SeNPs. The nanoparticles formed were characterized via various techniques i.e., UV-visible spectroscopy, FTIR, DLS, and TEM. The synthesized NL(C)-SeNPs were spherical with a size of 55 ± 7.0 nm. These capped SeNPs (NL(C)-SeNPs) show prominent bioactivity in terms of in-vitro anti-oxidant properties and anti-microbial activity on Escherichia coli, Enterobacter faecalis, and Staphylococcus aureus and antifungal activity on Aspergillus niger and Fusarium laterium. The results indicated NL(C)-SeNPs portray increased potential anti-oxidant and anti-microbial activity in a dose-dependent manner. Furthermore, their anti-cancer activity on the HepG2 cell line was also observed in a dose-dependent manner. However, additional studies related to the toxicity and synergistic effects of SeNPs, are required before their therapeutic applications
... NPP is displayed in Fig. 9A, indicating that the residuals are found on the diagonal straight line of collagen-NPs biosynthesis by the cell-free supernatant of Streptomyces plicatus strain NEAA-3. This demonstrates that the predicted data fit the experimental findings, confirming the model's accuracy 19,85 . Figure 9B demonstrated the relationship between the predicted and experimental collagen-NPs biosynthesis values. ...
... The green line represents the best lambda value (best lambda = 0.79). The blue line lies in the optimal zone between the minimum and maximum C.I., this indicates that the predicted data fits the experimental data well and confirming that no data transformation is required 19,88 . ...
Collagen nanoparticles (collagen-NPs) are promising biological polymer nanoparticles due to their exceptional biodegradability and biocompatibility. Collagen-NPs were bio-fabricated from pure marine collagen using the cell-free supernatant of a newly isolated strain, Streptomyces sp. strain NEAA-3. Streptomyces sp. strain NEAA-3 was identified as Streptomyces plicatus strain NEAA-3 based on its cultural, morphological, physiological properties and 16S rRNA sequence analysis. The sequence data has been deposited under accession number OR501412.1 in the GenBank database. The face-centered central composite design (FCCD) was used to improve collagen-NPs biosynthesis. The maximum yield of collagen-NPs was 9.33 mg/mL with a collagen concentration of 10 mg/mL, an initial pH of 7, an incubation time of 72 h, and a temperature of 35ºC. Using the desirability function approach, the collagen-NPs biosynthesis obtained after FCCD optimization (9.53 mg/mL) was 3.92 times more than the collagen-NPs biosynthesis obtained before optimization process (2.43 mg/mL). The TEM analysis of collagen-NPs revealed hollow sphere nanoscale particles with an average diameter of 33.15 ± 10.02 nm. FTIR spectra confirmed the functional groups of the collagen and the cell-free supernatant that are essential for the efficient capping of collagen-NPs. The biosynthesized collagen-NPs exhibited antioxidant activity and cytotoxic activity against HeP-G2, MCF-7 and HCT116 cell lines. Collagen-NPs assessed as nanocarrier with methotrexate (MTX), a chemotherapeutic agent, at pH values of 5.5 and 7.4. The TEM analysis revealed that the average size of MTX-loaded collagen-NPs was 35.4 ± 8.9 nm. The percentages of drug loading (DL%) and encapsulation efficiency (EE%) were respectively 22.67 and 45.81 %.
... Biogenically MgONPs may be synthesized by employing plants leaves, roots, flower and bark extracts as well as from bacteria (Acinetobacter johnsonii strain RTN1, Streptomyces coelicolor strain E72) [14,15], fungi (Aspergillus flavus) [16] and algae (Sargassum wighitii) [17]. However, biogenic synthesis of magnesium-oxide nanoparticles from plant is a preferred method because of cost effectiveness, high yield and ease of cultivation [18]. ...
At present, nanotechnology is developing at exponential rate because of its widespread applications in biomedical field. Traditionally, nanoparticles are fabricated through variety of physical and chemical approaches but because of their limited efficiency in life sciences, biogenic synthesis of nanoparticles has gained much attention. Biogenically fabricated MgONPs possess unique features that enabled their use in therapeutics and environmental biotechnology. Biogenically MgONPs can be fabricated by employing plant extracts (Manihot esculenta, Rhizophora lamarckii, Ocimum sanctum, Rosemary, Aloe vera, Orange, Artemisia abrotanum, Neem, Bauhinia purpurea), bacteria (S. coelicolor, A. johnsonii RTN), fungi (Aspergillus flavus TFR-12, Aspergillus brasiliensis TFR 2, white-button mushroom’s) and algae (S. whigti). These biogenically synthesized MgONPs are being reported as anti-fungal, antioxidant, antibacterial, anti-pyretic, anti-inflammatory and anti-cancer agents. Moreover, their photocatalytic activity is being discovered against certain organic dyes. This review focuses on biogenic synthesis of MgONPs, their applications in therapeutics and as antimicrobial agents as well as future prospects associated with their application in biomedical field.
... To assess each coefficient's significance and determine the pattern of the test variables' mutual interaction, the probability value of each coefficient was determined. Coefficients with smaller P-values (less than 0.05) are more significant 69,73 . With a P-value of less than 0.0001, the linear impacts of X 1 (pH), and X 4 (collagen concentration) and a P-value of 0.0005 for X 2 (temperature) demonstrated the maximum significance of these variables. ...
Collagen nanoparticles (collagen-NPs) are promising biopolymeric nanoparticles due to their superior biodegradability and biocompatibility. The low immunogenicity and non-toxicity of collagen-NPs makes it preferable for a wide range of applications. A total of eight morphologically distinct actinomycetes strains were newly isolated from various soil samples in Egypt. The cell-free supernatants of these strains were tested for their ability These strains' cell-free supernatants were tested for their ability to synthesize collagen-NPs. Five isolates had the ability to biosynthesize collagen-NPs. Among these, a potential culture, Streptomyces sp. NEAA-1, was chosen and identified as Streptomyces xinghaiensis NEAA-1 based on 16S rRNA sequence analysis as well as morphological, cultural and physiological properties. The sequence data has been deposited at the GenBank database under the accession No. OQ652077.1. Face-centered central composite design (FCCD) has been conducted to maximize collagen-NPs biosynthesis. Maximum collagen-NPs was 8.29 mg/mL under the condition of 10 mg/mL of collagen concentration, initial pH 7, incubation time of 48 h and temperature of 35ºC. The yield of collagen-NPs obtained via FCCD optimization (8.29 mg/mL) was 3.32-fold compared to the yield obtained under non-optimized conditions (2.5 mg/mL). TEM analysis of collagen-NPs showed hollow sphere nanoscale particles with mean of 32.83 ± 14.67 nm in diameter. FTIR spectra showed major peaks of amide I, amide II and amide III of collagen and also the cell-free supernatant involved in effective capping of collagen-NPs. The biosynthesized collagen-NPs exhibited anti-hemolytic, antioxidant and cytotoxic activities. The inhibitory concentrations (IC50) against MCF-7, HeP-G2 and HCT116 cell lines were 20.69 ±1.5, 24.48 ±1.9 and 15.61 ± 1.2 µg/mL; respectively. The in-vivo investigation showed that collagen-NPs can suppress Ehrlich ascites carcinoma (EAC) growth in mice. The collagen-NPs/DOX combination treatment showed considerable tumor growth suppression (95.58%). Collagen-NPs evaluated as nanocarrier with a chemotherapeutic agent, methotrexate (MTX). The average size of MTX loaded collagen-NPs was 42.37 ± 3.5 nm. Encapsulation efficiency percentage (EE %) was 48.91% and drug loading percentage (DL %) was 24.45%.
... The cytotoxic effect of the green-synthesized Ag-NPs on tumor cells is caused by the release of silver ions from the Ag-NPs, which results in interactions with cellular functional proteins, decreasing the viability and motility of the cells, as a molecule of DNA became more condensed. Furthermore, the generation of reactive oxygen species (ROS) and superoxide in mitochondria results in oxidative damage to DNA, proteins, and lipids, which ultimately leads to cell death [41,57,58]. ...
