Fig 1 - uploaded by Halla Abdul-Hadi Chabuk
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Transverse -section of the testicular tissue for Male Rats (control groups) showed the natural shape of the Seminiferous tubules and successive stages of the formation of sperm (Spermatogenesis) with a high level of sperm concentration Spermatozoa in the tubules cavity H.& E. (10 X).
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Toxoplasmosis is a zoonotic infection of animals caused by the protozoan parasite Toxoplasma gondii. It is one of the most important pathogens in humans and other warm-blooded animals that have an effect on reproductive function. The aim of this study has detected the effect of toxoplasmosis on some Physiological parameters and Histological structu...
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... current study showed changes in testicular tissue and epididymis tissue in animals infected with toxoplasmosis, may be reason to contact Tachyzoit with host cells and it penetrate these cells, leading to formation cysts, as a result, it divided and multiply within cysts and it continue in multiplication until the arrival of this number to a critical mass, Exploding the cell, which leads to apoptosis, necrosis this cells and severe inflammation in tissue, as well as the parasite secreted proteolytic enzyme, which analyzes cells and the presence of tissue cyst for years in various tissues of the body cause inflammation in the infected position (Topy et al, 1996). Also, we cannot seen Tachyzoiteby dyes routine tissue, but immune glow dye could show the parasite (Aghwan et al, 2010). ...
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Citations
... The results obtained in this study concerning sperm parameters were synonymous with the reports of Terpsidis et al. (2009), Abdoli et al. (2012, Chabuk (2019) and Tyebji et al. (2020). On the other hand, others (Moura et al., 2007;Abdulla et al., 2015) recorded no difference in the percentage of abnormal sperm between Toxoplasma-rats and control rats. ...
Infertility is a prevalent condition affecting approximately 70 million people worldwide and male factor contributes to about fifty percent of the issues. Studies on infectious agents as a possible cause of infertility has become prominent in the past decade. Toxoplasma gondii has emerged as a prime candidate as it has been found in the reproductive organs and semen of males of many animal species and humans. The aim of this study is to assess the effect of latent toxoplasmosis on experimental rat fertility. Ninety Toxoplasma infected rat were used as the experimental group besides, thirty control naïve ones. Both groups were observed clinically. Weekly assessment of fertility indices starting from the 7th week post infection till the 12th week were done by recording rat body weight, weight of testes, semen analysis and histo-morphometric analysis of the testes. Toxoplasma infected rats exhibited significant gradual loss of body weight and the absolute weight of the testes. The sperm characteristic parameters including percentage of motile sperm, percentage of viable sperm and sperm concentration in Toxoplasma infected rats showed highly significant decrease throughout the observation period in comparison to the control group with recording highly significant increase in the percentage of abnormal sperm forms. Pathological insults in tests of the infected rat group were denoted. Our findings demonstrated that Toxoplasma gondii is accused for affecting male rat main reproductive parameters and is implicated in the male reproductive disorders.
... The results obtained in this study concerning sperm parameters were synonymous with the reports of Terpsidis et al. (2009), Abdoli et al. (2012, Chabuk (2019) and Tyebji et al. (2020). On the other hand, others (Moura et al., 2007;Abdulla et al., 2015) recorded no difference in the percentage of abnormal sperm between Toxoplasma-rats and control rats. ...
Background: About one third of the human population is infected with Toxoplasma gondii, along with an over growing number of couples suffering from idiopathic infertility. There is lack about the possible role of Toxoplasma gondii infection in causing infertility in males to whom nearly 50% of the causes were attributed.
Objective: Providing novel insights into the associated morbidity in the testes and its reflection on semen analysis, which is considered as the main index of male fertility in clinical andrology.
Materials and Methods: Ninety Wistar male rats were infected with cystogenic type II Me49 Toxoplasma strain. Naïve (non-infected) male Wistar rats (30) were used as controls. From the 7th week post infection (time at which brain cysts are expected to be formed) until the 12th week, control (5) and Toxoplasma- infected (15) rats were subjected to weekly semen analysis and estimation of brain cyst burden.
Results: Sperm concentration and motility significantly decreased in Toxoplasma-infected rat groups compared to the control rat group. Moreover, abnormal sperm forms significantly increased in Toxoplasma-infected rats.
Conclusion: Our results demonstrated that Toxoplasma gondii had negative effects on sperm function and induced progressive deterioration of semen quality.
... These solutions were stirred, one drop was put on the slide, and a coverslip was put over the droplet. At least 10 microscopic fields were observed at 400x magnification to find the concentration of sperm (N) in 10 fields according to the following equation by Chabuk (2019). ...
... To evaluate the motility, one drop of the epididymis solutions was put on a clean slide to calculate the moving sperm percentage in 10 random fields according to the equation. After that, eosinnigrosin stains were used to stain the sperms to determine their morphology (Chabuk 2019). ...
The prevalence of testicular toxicity induced via contamination with heavy metals is increasing worldwide, and a close connection between testicular toxicity and lead has recently been identified. The present study demonstrated the possible therapeutic and protection properties of Nigella sativa oil (NSO) against dangerous testicular toxicity and sexual behaviour disorders in rat models induced by lead monoxide (PbO) food poisoning. A total of 24 male rats were used and divided into four groups, consisting of Group 1: Control (1 mL of distilled water); Group 2: PbO (80 mg/kg); Group 3: NSO (0.5 mL/rat); and Group 4: (NSO+PbO) 0.5 mL/rat of NSO, followed by PbO (80 mg/kg/day) respectively for 60 days. The testosterone, follicle-stimulating hormone (FSH), luteinizing hormone (LH), 17 beta-hydroxysteroid dehydrogenase activity (17β-HSD), sperm parameters, and sexual behaviour (copulation behaviour test) were measured. The results showed that sex hormone levels were significantly decreased (p<0.05) in rats exposed to PbO, while the levels of these hormones were significantly higher in rats exposed to NSO and NSO+PbO. The sperm parameters (numbers, concentrations, motilities, and abnormality) were lower in rats exposed to PbO compared to those in the control, NSO, and NSO+PbO groups that showed significant increases. Sperm abnormality and 17β-HSD activity showed a significant increase at p<0.05 in the group exposed to the PbO compared to those of the control, NSO, and NSO+PbO groups. The results also significantly suppressed sexual behaviour in rats exposed to PbO compared to those in the control, NSO, and NSO+PbO groups, rats were administered NSO+PbO showed improvement in sexual behaviour parameters compared to PbO groups. PbO toxicity was obvious in the reproductive tract through severe histopathological changes. The impact of lead on tissues was partially mitigated by NSO. The study showed protective effects of N. sativa oil against changes in sex hormones, sperm characteristics, and sexual behaviour in male rats exposed to lead monoxide-induced testicular toxicity.
... But after 12 weeks, PC had the greatest effect, such as congestion, SH, and bleeding in the blood vessels between the tubules by reducing the size of these tubules and witnessing necrosis and loss of most epithelial layers of epididym and reducing the size of the epididymal tubes. We also saw infection after 6 weeks, and the epididymal cavity was empty of sperm [31]. ...
