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The aim of this study was to evaluate the morphology of the species Sticholecitha serpentis Prudhoe, 1949 by means of histological procedures. Helminths were previously fixed in Railliet & Henry solution, uncompressed and were included in 2-hydroxyethyl-methacrylate. Longitudinal and transverse serial sections with a 4-mum thickness were performed...
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... The P. gracile ovary was located near the posterior sucker similar to other rumen flukes [9]. In comparison to other trematodes whose posterior sucker is present in ventral area of the middle body, the ovaries were located near the posterior sucker [25,26]. In P. gracile, oogenesis in the tubules of the ovary includes at least four stages of development [27]. ...
As the aim of this study, we evaluated the histological morphology of adult Paramphistomum gracile. Adult flukes of which the bodies were 5-15 mm in length and 2-7 mm in width were examined by using a histological analysis. Longitudinal and transversal serial-sections that were stained with haematoxylin & eosin solution were described. The body surface and longitudinal section of P. gracile were also observed by using a scanning electron microscopy (SEM). In this species, the anterior sucker and posterior sucker (acetabulum) presented on an anterior and posterior part, respectively. The major folds were found at the areas of anterior sucker, genital canal, and posterior sucker. In fluke membrane, there was spineless at tegument surface and in tegument tissue. Histological data showed structural-systematic value of digestive tract, reproductive tract, excretory tract, copulatory organs, connective tissues and muscle tissues. Here we attempted to clarify the histological profiles of P. gracile that might contribute to increasing the knowledge and understanding of morphology in rumen fluke.
The present work aims to expand the knowledge of the digenean species Prosogonotrema bilabiatum (Sclerodistomidae), a parasite of Chaetodipterus faber (Acanthuriformes) from Brazil, with an integrative taxonomic approach, using light microscopy, scanning electron microscopy, histology, and molecular biology.
Forty-one digenean specimens were stained with hydrochloric carmine for morphological studies. Eleven parasites were dehydrated through a graded ethanol series, critical point dried with carbon dioxide, and coated with gold for scanning electron microscopy analysis. Four specimens were processed following histological routine and stained with hematoxylin and eosin and Gomori trichrome. DNA extracted was amplified using 28S partial primer D1–D3. Maximum likelihood and Bayesian inference were performed for phylogenetic analysis.
Morphometric and morphological data of the specimens studied ranged in accordance as observed in previous descriptions of the species. Observations from scanning electron microscopy and histology corroborated with those observed in stained whole mounts. Molecular analysis showed that specimens of P. bilabiatum from Brazil clustered with another two sequences of this species from different hosts and localities, with a high node support value.
The integrative taxonomic approach allowed to record and describe new characteristics of P. bilabiatum related to the tegument, the structure and the arrangement of its tissues. The use of molecular markers confirmed that specimens identified as P. bilabiatum from different hosts and localities are all conspecific. Further studies, mainly molecular with less conserved genetic markers, should be carried out to better understand the phylogenetic relationships of Prosogonotrema with Hemiuroidea.
