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The stem loop structure of the candidate Novel miRNAs. a is the stem loop structure of unconservative_ST4.03ch04_13453; b is the stem loop structure of unconservative_ST4.03ch05_20491; c is the stem loop structure of unconservative_ST4.03ch06_24957; d is the stem loop structure of unconservative_ST4.03ch11_40149
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Low-temperature is one of the most severe abiotic stresses affecting the potato production as the cultivated potato (Solanum tuberosum) is frost sensitive. MicroRNAs have been identified in response to low-temperature stress in plants. Here, via high throughput sequencing, we described the profiling of low-temperature stress response to miRNA and m...
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The refrigerated induce the sweetening of the potato, which results in the dark color. The objective of this study was to determine the temperature and shelf life of Markies potato tubers under refrigerated storage for the processing of French fries. The levels of total soluble sugar (TSS), reducing sugars (RS), peroxidase (POD) and polyphenoloxida...
Abstract This work aimed to analyze 1,4-DMN efficiency in the suppression of sprouting and the consequent maintenance of quality in potato tubers of Asterix and Challenger cultivars stored during different periods under varying temperatures: eight treatments with and without 1,4-DMN under temperatures of 8 °C and 20 °C. Treatments effect were evalu...
Citations
... miR-96 is upregulated in CRC, and it plays a role in downregulating p53 by targeting Tumor Protein P53 Inducible Nuclear Protein 1 (TP53INP1) (Gao and Wang 2015). miR-34a increases the activity of p53 by inhibiting Sirtuin 1 (SIRT1) (Yan et al. 2021). miR-320a directly targets SRY-Box Transcription Factor 4 (SOX4) and Forkhead Box M1 (FOXM1) and exerts a cancer-suppressive effect on CRC (Vishnubalaji et al. 2016). ...
This study is aimed at investigating the roles of Toll-like receptor 4 (TLR4) and microRNA-7 (miR-7) in colorectal cancer (CRC) development and progression. We assessed TLR4 and miR-7 expression in CRC cells and tissues using reverse transcription-quantitative polymerase chain reaction. The relationship between miR-7 and TLR4 was analyzed through dual luciferase reporter assays. MTT, wound healing, and cell invasion assays were conducted to examine the effects of TLR4 and miR-7 on CRC cell proliferation, migration, and invasion. Western blotting was used to explore the involvement of the TRAF6/NF-κB signaling pathway. miR-7 was underexpressed in CRC, while TLR4 levels were increased. miR-7 negatively regulated TLR4 expression and its knockdown enhanced CRC cell proliferation, migration, and invasion. TLR4 knockdown had the opposite effects. The TRAF6/NF-κB pathway was linked to TLR4’s role in tumor progression. miR-7 might inhibit TRAF6/NF-κB target a signaling pathway of TLR4 and promote CRC occurrence. miR-7 may therefore be used as a sensitive biomarker in CRC patients.
... There are RNAs in nature that are non-coding, small (21-24 nucleotides), endogenous, and highly conserved across species which are involved in the regulation of plant growth, signaling, development and activate response to external environmental factors including drought stress (Pagano et al. 2021). Such RNAs are known as microRNAs (miRNAs), several studies indicate that miRNAs play an active role in response to abiotic stress in different plants including peanuts , sunflower (Liang et al. 2020), pigeon peas (Buch et al. 2020), potato (Liao et al. 2021), Arabidopsis (Fasani et al. 2021) and tea (Zhu et al. 2021). ...
Drought stress is a significant environmental factor that adversely affects the growth and development of carrot
(Daucus carota L.), resulting in reduced crop yields and quality. Drought stress induces a range of physiological
and biochemical changes in carrots, including reduced germination, hindered cell elongation, wilting, and disrupted
photosynthetic efficiency, ultimately leading to stunted growth and decreased root development. Recent research
has focused on understanding the molecular mechanisms underlying carrot’s response to drought stress, identifying
key genes and transcription factors involved in drought tolerance. Transcriptomic and proteomic analyses have
provided insights into the regulatory networks and signaling pathways involved in drought stress adaptation. Among
biochemical processes, water scarcity alters carrot antioxidant levels, osmolytes, and hormones. This review provides
an overview of the effects of drought stress on carrots and highlights recent advances in drought stress-related
studies on this crop. Some recent advances in understanding the effects of drought stress on carrots and developing
strategies for drought stress mitigation are crucial for ensuring sustainable carrot production in the face of changing
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affected by drought.
... miRNA*s and 34 targets for two particular potato genotypes to cold storage treatment regarding the decrease in sugar deposition, indicating a potential role for miRNAs in cold-induced sweetening. Recently, another study group has reported the profiling of miRNA and target mRNAs in potato plants, Favorita var., using high-throughput sequencing under low temperature stress (0°C 12 h) by creating two RNA libraries and six mRNA libraries [97]. There were 294 known and 211 novel miRNAs identified, where 24 miRNAs showed higher expression in the low temperature-treated group than controls, and 80 miRNAs displayed lower expression in treated plants compared to control plants. ...
Potato (Solanum tuberosum L.) is one of the essential non-cereal crops with noticeably greater production and consumption rates across the world. Because of the diverse range of utilization of nutritious tubers, potatoes can be used as an alternative food to address regional, national, and global food security issues compromised by global climate change. Since potato yield and quality are dramatically affected by abiotic stress conditions, the development of potato plants showing greater performance after being exposed to individual or combined stress treatments has become intriguing for the past decade. In this book chapter, recent studies and/or approaches associated with potato genomics, transcriptomics, and identification of miRNAs are summarized to discuss the response of potatoes to abiotic stress factors in different regulatory levels. Such a summary should encompass the importance and identification of factors for the development of potato plants under current and potential exacerbating effects caused by abiotic stress.
... chilling damage at -0.8 ℃, frost damage at -2 ℃, death at -3 ℃ [5]. At the same time, low temperature also has a series effects on the metabolism of malondialdehyde (MDA) [6], proline (Pro) [7], soluble sugar [8] and other substances [9], and lead to changes in molecular metabolism and subsequent physiological metabolism. However, the specific molecular mechanisms by which potato responds to low-temperature are not known and therefore research is urgently needed. ...
Background
Potato (Solanum tuberosum L.) is one of the world's most important crops, the cultivated potato is frost-sensitive, and low-temperature severely influences potato production. However, the mechanism by which potato responds to low-temperature stress is unclear. In this research, we apply a combination of second-generation sequencing and third-generation sequencing technologies to sequence full-length transcriptomes in low-temperature-sensitive cultivars to identify the important genes and main pathways related to low-temperature resistance.
Results
In this study, we obtained 41,016 high-quality transcripts, which included 15,189 putative new transcripts. Amongst them, we identified 11,665 open reading frames, 6085 simple sequence repeats out of the potato dataset. We used public available genomic contigs to analyze the gene features, simple sequence repeat, and alternative splicing event of 24,658 non-redundant transcript sequences, predicted the coding sequence and identified the alternative polyadenylation. We performed cluster analysis, GO, and KEGG functional analysis of 4518 genes that were differentially expressed between the different low-temperature treatments. We examined 36 transcription factor families and identified 542 transcription factors in the differentially expressed genes, and 64 transcription factors were found in the AP2 transcription factor family which was the most. We measured the malondialdehyde, soluble sugar, and proline contents and the expression genes changed associated with low temperature resistance in the low-temperature treated leaves. We also tentatively speculate that StLPIN10369.5 and StCDPK16 may play a central coordinating role in the response of potatoes to low temperature stress.
Conclusions
Overall, this study provided the first large-scale full-length transcriptome sequencing of potato and will facilitate structure–function genetic and comparative genomics studies of this important crop.
... Further, with the advent of high-throughput sequencing, the existing repository for miRNAs, the potential regulators of gene expression, mainly via post-transcriptional gene silencing, have been expanded across the plant kingdom. The involvement of miRNAs in response to varied environmental cues like salinity, drought, cold, and nutrient deficiency have been reported in several plants, like Arabidopsis (Fasani et al. 2021), tea , potato (Liao et al. 2021), sunflower (Liang et al. 2020), including legumes such as, grass pea (Bhat et al. 2020), pigeon pea (Buch et al. 2020), and peanut (Ren et al. 2020). So far cowpea is concerned, only Barrera-Figueroa et al. (2011) have performed miRNA profiling in response to drought stress in the cultivars contrast for drought tolerance. ...
Key message
Drought stress response studies and overexpression of vun-miR408 proved it to be essential for abiotic stress tolerance in cowpea.
Abstract
Small RNA and transcriptome sequencing of an elite high-yielding drought-tolerant Indian cowpea cultivar, Pusa Komal revealed a differential expression of 198 highly conserved, 21 legume-specific, 14 less-conserved, and 10 novel drought-responsive microRNAs (miRNAs) along with 3391 (up-regulated) and 3799 (down-regulated) genes, respectively, in the leaf and root libraries. Among the differentially expressed miRNAs, vun-miR408-3p, showed an up-regulation of 3.53-log2-fold change under drought stress. Furthermore, laccase 12 (LAC 12) was identified as the potential target of vun-miR408-3p using 5′ RNA ligase-mediated rapid amplification of cDNA ends. The stable transgenic cowpea lines overexpressing artificial vun-miR408-3p (OX-amiR408) displayed enhanced drought and salinity tolerance as compared to the wild-type plants. An average increase of 30.17% in chlorophyll, 26.57% in proline, and 27.62% in relative water content along with lesser cellular H2O2 level was observed in the transgenic lines in comparison with the wild-type plants under drought stress. Additionally, the scanning electron microscopic study revealed a decrease in the stomatal aperture and an increase in the trichome density in the transgenic lines. The expression levels of laccase 3 and laccase 12, the potential targets of miR408, related to lipid catabolic processes showed a significant reduction in the wild-type plants under drought stress and the transgenic lines, indicating the regulation of lignin content as a plausibly essential trait related to the drought tolerance in cowpea. Taken together, this study primarily focused on identification of drought-responsive miRNAs and genes in cowpea, and functional validation of role of miR408 towards drought stress response in cowpea.
... It was similar to previous reports on tomato and pepper. The expression levels ranged from a few to thousands and different conserved miRNAs had different expression levels (Cao et al., 2014;Din et al., 2016;Liu M. et al., 2017;Liao et al., 2021). However, a lot of novel miRNAs in potato had a higher expression level, with reads of more than 100, which was different from the previous studies in tomato and Arabidopsis (Fahlgren et al., 2007). ...
In recent years, with the wanton destruction of the ecological environment by humans and the frequent occurrence of extreme bad weather, many places that should have been warm and blooming in spring have instead experienced the phenomenon of the “April blizzard,” which has seriously affected China's crops, especially spring potato production in most areas. Potato cultivars, especially potato seedlings, are sensitive to frost, and low temperature frost has become one of the most important abiotic stresses affecting potato production. Potato cold tolerance is regulated by a complex gene network. Although some low temperature resistant microRNAs have been identified, little is known about the role of miRNAs in response to low temperature stress in potato. Therefore, the objective of this study is to clarify the influence of low temperature stress on the miRNA expression of potato by comparing the expression differences of miRNA in potato which was treated with different low temperatures. For the study, 307 known miRNAs belonging to 73 small RNA families and 211 novel miRNAs were obtained. When the temperature decreased, the number of both known and novel miRNA decreased, and the minimum temperature was −2°C. Most of the miRNAs respond to low temperature, drought, and disease stress; some conserved miRNAs were first found to respond to low temperature stress in potato, such as stu-miR530, stu-miR156d, and stu-miR167b. The Gene Ontology, Kyoto Encyclopedia of Genes, and Genomes pathway enrichment analysis of 442 different expression miRNAs target genes indicated that there existed diversified low temperature responsive pathways, but Abscisic Acid was found likely to play a central coordinating role in response to low temperature stress in many metabolism pathways. Quantitative real-time PCR assays indicated that the related targets were negatively regulated by the tested different expression miRNAs during low temperature stress. The results indicated that miRNAs may play an important coordination role in response to low temperature stress in many metabolic pathways by regulating abscisic acid and gibberellin, which provided insight into the roles of miRNAs during low temperature stress and would be helpful for alleviating low temperature stress and promoting low temperature resistant breeding in potatoes.
Solanaceous vegetables are major group contributing for the food security, nutritional quality, and economic empowerment of farmers. Recent climatic changes affect the production and productivity by enhanced sensitivities to biotic and abiotic stresses. Developments in the field of structural and functional genomics, proteomics facilitate the understanding of mechanism of tolerance to biotic and abiotic stresses. Prebreeding lines have been characterized through single-nucleotide polymorphism diversity analysis, and traits were finely mapped. Functional genomics approaches widely utilized for transformation with candidate genes to enhance tolerance to stresses as well as keeping quality. In this chapter, omics concepts, namely genomics, proteomics, transcriptomics, and metabalomics, playing important roles in understanding the mechanism of tolerances against biotic and abiotic stresses, genome organization and evolution of plants species, and utilization in development of crop varieties with novel traits have been discussed. Advances in the omic era elucidated the genome structures of economically important crops, namely rice, tomato, maize, etc., which demonstrated the characterization of various economically important genes and successful utilization in crop improvement programs.
