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A single PCR protocol for methicillin-resistant S. aureus (MRSA) housekeeping genes, carbamate kinase, shikimate dehydrogenase, glycerol kinase, guanylate kinase, phosphate acetyltransferase, triosephosphate isomerases, and acetyl coenzyme-A acetyltransferase for multilocus sequence typing was developed. This PCR strategy provides a simple, rapid a...
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... in a single PCR setup produced distinct bands, corresponding to their respective molecular sizes that were recognizable in stained agarose gels. Explicit and high yield products were obtained by using newly developed TD-PCR approach. In addition, two different cycles of annealing temperature were adjusted so that the aforementioned primers (Enright et al., 2000) could be used for all S. aureus MLST loci. Each allele of all S. aureus strains were amplified satisfactorily by using new TD-PCR strategy (Figure 2). The characterization of pathogenic isolates plays an essential role in the epidemiology of infectious diseases, generating the necessary information for identification, tracking, and intervening against disease outbreaks. MLST has been applied to all the major human ...
