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The secretory pathway for protein synthesis and sorting Proteins are synthesized and transferred into the lumen of the ER. From there the proteins are transported by vesicles to the cis-Golgi. The proteins go through the Golgi and proteins destined to the vacuole are sorted and packaged at the TGN into clathrin coated vesicles. These vesicles are transported to PVC before they reach the vacuole. Proteins determined for exocytosis are transported by vesicles to the plasma membrane where they fuse and release their contents into the extracellular space. Endocytotic vesicles budding off from the plasma membrane are transported to the PVC (Buchanan et al., 2000).
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Alle eukaryontischen Zellen benötigen für die Sekretion ein funktionierendes endomembranes System. Proteine, die sekretiert werden besitzen ein Signalpeptid und werden am Endoplasmatischen Retikulum (ER) synthetisiert. Das Signalpeptid initiiert den Transport in den Lumen des ER und wird danach enzymatisch vom Protein abgetrennt. Korrekt gefaltete...
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... of the cell wall in a growing plant cell needs a continuous production of cell wall precursors and the corresponding enzymes. These components are transported by vesicles and secreted in the extracellular space where the formation of the cell wall takes place ( Figure 2). ...
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... interaction to occur in vivo, SNP33 and KN need to be ex- pressed in overlapping domains. To test for SNP33 expression, we generated a polyclonal antiserum against the full-length pro- tein. The antiserum recognized different forms of recombinant SNP33 protein but not the related AtSNAP29 (Fig. 2 A). On Western blots of plant extracts, a band corresponding to the predicted size of SNP33 was detected. This band was only present in wild-type extracts but not in extracts from a snp33 T-DNA insertion mutant (see below), confirming the specific- ity of the anti-SNP33 antiserum (Fig. 2 ...
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... of recombinant SNP33 protein but not the related AtSNAP29 (Fig. 2 A). On Western blots of plant extracts, a band corresponding to the predicted size of SNP33 was detected. This band was only present in wild-type extracts but not in extracts from a snp33 T-DNA insertion mutant (see below), confirming the specific- ity of the anti-SNP33 antiserum (Fig. 2 ...
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... SNP33 protein was detected in snp33 mutant tissue (Fig. 2) even after overexposure of the Western blots, in- dicating that the mutant represents a complete loss of function. Thus, residual gene function does not account for the rather weak cytokinetic phenotype of snp33 mutants. Sequencing of the Arabidopsis genome identified two ad- ditional SNAP25 homologous genes, AtSNAP29 (AGI-ID: ...
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... RNA gel stained with ethidium bromide is shown as a control for loading. Figure 2 shows the kinetic of expression of AtSNAP33 and PR1 after inoculation of A. thaliana Col-0 with the downy mildew pathogen P. parasitica isolate NOCO and EMWA. The isolate NOCO forms a compatible interaction and the isolate EMWA an incompatible interaction with A. thaliana accession Columbia (Parker et al., 1993). ...
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... the atsnap33 mutant the defence responses were up regulated. The pathogenesis-related proteins PR-1 and PR-2 as well as the defensin PDF1.2 were expressed in 7 days old atsnap33 plantlets as shown by Northern blot analysis ( Figure 2E). ...
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... level of transcripts of AtSyp122 and AtSyp43 was analysed in different tissues of flowering plants and in roots grown in vitro (Figure 2). RNA hybridisation analysis was performed with a probe in a non conserved part of the sequence (see Figure 1A and 1B). ...
