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The relative quantification of Lactobacillus species in UC patients. The fold change was calculated using log10 RQ where RQ is 2−ΔΔCt. Statistical significance was determined by a t-test comparing ΔCt of healthy controls to ΔCt of UC patients. *P < 0.05.
Source publication
Aim:
To analyze the changes of different Lactobacillus species in ulcerative colitis patients and to further assess the therapeutic effects of selected Lactobacillus strains on dextran sulfate sodium (DSS)-induced experimental colitis in BALB/c mice.
Methods:
Forty-five active ulcerative colitis (UC) patients and 45 population-based healthy cont...
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Background
Ulcerative colitis (UC) is a common inflammatory intestinal disease. Astragali Radix (AR) is one of the traditional Chinese medicines used in clinic for UC treatment. In our previous study, the whole ingredient extract (WIE) from AR have been proved to possess better immunomodulatory effects on immunosuppressed mice compared with the tra...
Background:
Endometrial regenerative cells (ERCs) have been identified to ameliorate colitis in mice; however, whether exosomes derived from ERCs (ERC-exos) own similar effects on colitis remains unclear. Ferroptosis, an iron-dependent cell programmed death form, has been reported to promote inflammation in UC. Thus, in this study, whether ERC-exo...
Abstract Background Dextran sulfate sodium (DSS) replicates ulcerative colitis (UC)-like colitis in murine models. However, the microbial characteristics of DSS-triggered colitis require further clarification. To analyze the changes in gut microbiota associated with DSS-induced acute and chronic colitis. Methods Acute colitis was induced in mice by...
Accumulating evidence suggests that the gut microbiota plays an important role in the pathogenesis of inflammatory bowel disease (IBD). Carnosic acid (CA) is a major antioxidant component of rosemary and sage. Herein, we investigated the protective effects of dietary CA on dextran sodium sulfate (DSS)-induced colitis mouse model with an emphasis on...
Ulcerative colitis (UC) is a type of inflammatory bowel disease characterized by inflammation of the large intestine, rectal bleeding, and abdominal pain. It can be alleviated by certain bioactive compounds, including α-linolenic acid (ALA), which is a bioactive component in fermented black radish (Raphanus sativus L. var. niger). The aim of this s...
Citations
... A total of 2,707 studies were obtained, 291 studies duplicates were removed, 2,273 studies were excluded after reading the title/ abstract, and 65 studies were retained after reading the full text according to the PICO inclusion criteria, and then, through further screening, 1 study was repeatedly published, we were unable to obtain the full text from 12 studies, and 10 studies had incomplete data. Finally, 42 studies were included in the network meta-analysis Zakostelska et al., 2011;Chen et al., 2012;Chen et al., 2013;Pan et al., 2014;Elian et al., 2015;Cui et al., 2016;Kanda et al., 2016;Liu et al., 2016;Chae et al., 2018;Sun et al., 2018;Wang et al., 2018;Bian et al., 2019;Chae et al., 2019;Chen et al., 2019;Choi et al., 2019;Din et al., 2020;He et al., 2020;Hu et al., 2020;Sun et al., 2020;Yeo et al., 2020;Yu et al., 2020;Chen et al., 2021;Gao et al., 2021;Hu et al., 2021;Huang et al., 2021;Qu et al., 2021;Tong et al., 2021;Wang et al., 2021;Dong et al., 2022;Huang et al., 2022;Islam et al., 2022;Khan et al., 2022;Lee et al., 2022;Li et al., 2022;Lu et al., 2022;Ma et al., 2022;Qin et al., 2022;Shang et al., 2022;Wan et al., 2022;Wu et al., 2022). The specific selection flow chart was shown in Figure 1. ...
... There were 839 ulcerative colitis animal models, including 460 in the intervention group and 379 in the control group. There were four different kinds of mice, and 14 studies used the BALB/c mice (Lee et al., 2022;Chen et al., 2013;Liu et al., 2016;Pan et al., 2014;Liu et al., 2011;Kanda et al., 2016;Cui et al., 2016;Chen et al., 2019;Zakostelska et al., 2011;Elian et al., 2015;Sun et al., 2018;Yu et al., 2020;Wang et al., 2018;, with a total of 306 mice, 24 studies used the C57BL/ 6 mice (Chen et al., 2012;Chae et al., 2018;Bian et al., 2019;Chae et al., 2019;Din et al., 2020;He et al., 2020;Hu et al., 2020;Yeo et al., 2020;Chen et al., 2021;Gao et al., 2021;Hu et al., 2021;Huang et al., 2021;Qu et al., 2021;Tong et al., 2021;Dong et al., 2022;Huang et al., 2022;Islam et al., 2022;Khan et al., 2022;Lu et al., 2022;Ma et al., 2022;Qin et al., 2022;Shang et al., 2022;Wan et al., 2022;Wu et al., 2022), with a total of 436 mice, 2 studies used the ICR mice (Sun et al., 2018;Choi et al., 2019), with a total of 32 mice, 1 study used the Kunming mice , with 35 mice in total, and 1 study used the Sprague Dawley (SD) mice , with a total of 20 mice. All studies adopted animal models of ulcerative colitis induced by dextran sulfate sodium (DSS), and the administration duration ranged from 7 days to 35 days. ...
... bifidum) (Elian et al., 2015;Chae et al., 2018;Wang et al., 2018;Din et al., 2020;Dong et al., 2022;Lee et al., 2022;Shang et al., 2022), thirteen studies used Lactobacillus plantarum (L. plantarum) Cui et al., 2016;Chae et al., 2019;Choi et al., 2019;Sun et al., 2020;Yu et al., 2020;Hu et al., 2021;Huang et al., 2021;Wang et al., 2021;Khan et al., 2022;Qin et al., 2022;Wu et al., 2022), two studies used Akkermansia muciniphila (AKK) (Bian et al., 2019;Qu et al., 2021), two studies used Lactobacillus fermentum (L. fermentum) (Cui et al., 2016;Chen et al., 2021), four studies used Lactobacillus rhamnosus (L. ...
The imbalance of gastrointestinal microbial composition has been identified as the main factor of chronic inflammatory diseases. At present, probiotics have a beneficial effect on the microbial composition of the human gastrointestinal tract, but it is still controversial and the specific mechanism is unknown. The purpose of this network meta-analysis is to compare the mechanism of different probiotics on ulcerative colitis. PubMed, Embase, and Web of Science were searched till 16 November 2022. The SYRCLE risk bias assessment tool was used to assess the quality of the research studies. A total of 42 studies, 839 ulcerative colitis models, and 24 kinds of probiotics were finally included. The results showed that L. rhamnosus has the best effect in relieving weight loss and improving the Shannon index in the ulcerative colitis model. E. faecium has the best effect in reducing colon injury; L. reuteri has the best effect in reducing the DAI; L. acidophilus has the best effect in reducing the HIS index and increasing the expression of tight junction protein ZO-1; and L. coryniformis has the best effect in reducing the content of serum pro-inflammatory factor TNF-α. It indicated that probiotics can improve ulcerative colitis by improving histopathological manifestations, reducing inflammatory reaction, and repairing the mucosal barrier, and different probiotics showed different effects. However, considering the limitations of this study, preclinical studies that require more large samples and high-quality and more reliable and rigorous experimental designs and reports need to be conducted in the future.
Systematic Review Registration: https://www.crd.york.ac.uk/prospero/#record details, identifier CRD42022383383.
... Notably, probiotic bacteria strains have shown different effects on the severity of IBD in an experimental model. While Lactobacillus reuteri BR11 has reduced the severity of experimental IBD [27], L.crispatus CCTCC M206119 aggravated it [28]. On the other hand, L. plantarum NCIMB8826 treatment has led to weight gain and improved colon length without a remarkable effect on the disease activity index and histologic damage in the colitis model [28]. ...
... While Lactobacillus reuteri BR11 has reduced the severity of experimental IBD [27], L.crispatus CCTCC M206119 aggravated it [28]. On the other hand, L. plantarum NCIMB8826 treatment has led to weight gain and improved colon length without a remarkable effect on the disease activity index and histologic damage in the colitis model [28]. Interestingly, probiotic administration caused a reduction in the expression of proinflammatory cytokines and increased intestinal mucin-like and zona occludens-1 expression [29]. ...
Background:
Despite the number of available therapies for ulcerative colitis (UC), severe side effects and high cost has limited their clinical application. Thus, finding new alternative strategies with minimal side effects is inevitable. Therefore, this study aimed to compare the effectiveness of different therapeutic approaches in DSS-induced colitis.
Methods:
Firstly, we designed oral bio-therapeutic products, Live Bacterial Products (LBP), which include a mixture of fecal bacteria strains isolated from healthy mice and prepared by microencapsulation and freeze-dried techniques. Then we investigated the efficiency of 7 days of freeze-dried FMT, LBP, and vancomycin treatments in DSS-induced colitis. Secondly, we compared the effect of 15 days of microbial therapies (freeze-dried powder of FMT and LBP microcapsules) and seven days of oral vancomycin on the severity of colitis in mice. Furthermore, the levels of IL-1β and TNF-α were measured in serum by ELISA, and the fecal microbiota diversity was analyzed by high-throughput sequencing for all mice groups.
Results:
After seven days of treatments, our results indicated that oral vancomycin reduced the severity of DSS-induced colitis in mice, where weight gain and a decrease in IL-1 β and TNF-α levels were observed in the vancomycin group compared with other treatment groups. While after two weeks of treatment, the LBP microcapsules were able to reduce the severity of colitis. And at the end of the treatment period, weight gain and a decrease in the DAI scores and the levels of IL-1β and TNF-α were noted in the LBP treatment group compared to other treatment groups. By high-throughput sequencing of the 16S rRNA gene, our results showed that while the microcapsules LBP treatment increased the fecal microbial diversity, after vancomycin therapy, most of the fecal microbiota genera and operational taxonomic units (OTUs) were depleted.
Conclusion:
Our results concluded that treatment duration and preparation methods affect the microbial therapies' efficiency in UC. Furthermore, this study highlighted the negative consequences of oral vancomycin administration on gut health that should be known before using this medication.
... Conversely, L. acidophilus FGSYC48L79 exacerbated the symptoms of colitis. In previous studies, it had been found that L. crispatus CCTCC M206119 aggravated colitis in mice and further damaged the intestinal barrier [10]; Anaerostipes hadrus BPB5, a candidate probiotic strain, aggravated the disease activity index and mortality in DSS-induced colitis mice [11]. Such results alert us that some probiotics may not exert positive effects on individuals with disease or gut microbiota imbalance. ...
Inflammatory bowel disease (IBD) is a worldwide chronic intestinal inflammatory immune-related disease. In this study, mice with dextran sulfate sodium (DSS)-induced colitis were used to evaluate the effect of Lactobacillus acidophilus on colitis. The results revealed that L. acidophilus CCFM137 and FAHWH11L56 show potential for relieving colitis symptoms, while L. acidophilus FGSYC48L79 did not show a protective effect. Moreover, L. acidophilus NCFM and FAHWH11L56 showed similar effects on various indicators of DSS-induced colitis, increasing the IL-10 and IL-17 in the colon, and modifying the CCL2/CCR2 axis and CCL3/CCR1 axis. For L. acidophilus CCFM137, its effects on colitis were different from the above two strains. Moreover, L. acidophilus FGSYC48L79 had negative effects on colitis by increasing the abundance of harmful bacteria in the gut microbiota and may promote the signaling of chemokines and their receptors. This may be related to its special genome compared to the other strains.
... Many studies on the supplementation of Lactobacillus strains have focused on ameliorating colitis by regulating cytokines and alleviating tissue damages. L. casei protected mucosa damage and modulated the immune response against intestinal inflammation in DSS-treated mice and L. plantarum increased the induction of regulatory T cells and type 2 helper T cells in the spleen and suppressed pro-inflammatory cytokines e.g., TNF-α, IL-17 and IL-1β [41,42]. In addition, LAB-fermented bread showed high levels of phenolics and inhibition of the secretion of pro-inflammatory cytokines in vitro [43]. ...
The fruit of Prunus mume (PM) is widely cultivated in East Asia, and it has been used as a folk medication for gastrointestinal disorders, e.g., diarrhea, stomach ache and ulceration. In this study, the pectinase-treated PM juice (PJ) was fermented with Lactobacillus strains containing fundamental organic acids and free amino acids. The PJ fermented with Lactobacillus plantarum and L. casei (FP) was investigated for its protective effect in dextran sodium sulfate (DSS)-induced colitis mice model. The administration of FP reduced lipid peroxidation and histopathological colitis symptoms, e.g., shortening of the colon length, depletion of mucin, epithelial injury and ulceration, in colonic tissues. The FP-supplemented group showed the alleviation of pro-inflammatory cytokines. Compared with the DSS control group, the supplementation of FP significantly reduced the levels of serum interferon-γ (IFN- γ), interleukin (IL)-1β, IL-6, IL-12 and IL-17 as well as colonic tumor necrosis factor-α, IFN-γ, IL-12 and IL-17. Furthermore, the DSS-induced TUNEL-positive area was significantly reduced by the FP supplementation. These results show that the supplementation of FP fermented with mixed lactic acid bacteria, L. plantarum and L. casei, elucidated the protective effect in DSS-induced colitis mice. Hence, this study suggests that FP can be utilized as a natural therapeutic agent for colitis and intestinal inflammation.
... However, therapeutic strategies, including probiotic therapy and FMT, have been reported to cause unexpected results and serious safety concerns in a few recently implemented trials [20]. For instance, there was a report in which Lactobacillus, generally known to have beneficial effects on enteric diseases, aggravated enteric inflammation, depending on the species used [21]. Furthermore, a patient suffering from acute pancreatitis died after receiving an injection of probiotics due to bowel ischemia [22]. ...
Since the intimate relationship between microbes and human health has been uncovered, microbes have been in the spotlight as therapeutic targets for several diseases. Microbes contribute to a wide range of diseases, such as gastrointestinal disorders, diabetes and cancer. However, as host-microbiome interactions have not been fully elucidated, treatments such as probiotic administration and fecal transplantations that are used to modulate the microbial community often cause nonspecific results with serious safety concerns. As an alternative, synthetic biology can be used to rewire microbial networks such that the microbes can function as therapeutic agents. Genetic sensors can be transformed to detect biomarkers associated with disease occurrence and progression. Moreover, microbes can be reprogrammed to produce various therapeutic molecules from the host and bacterial proteins, such as cytokines, enzymes and signaling molecules, in response to a disturbed physiological state of the host. These therapeutic treatment systems are composed of several genetic parts, either identified in bacterial endogenous regulation systems or developed through synthetic design. Such genetic components are connected to form complex genetic logic circuits for sophisticated therapy. In this review, we discussed the synthetic biology strategies that can be used to construct engineered therapeutic microbes for improved microbiome-based treatment.
... Our results highlighted that DSS-treated mice supplemented with biofilm-like microcolonies encased in the pectin beads displayed a decrease in colonic mucosal injuries, lower inflammation and reduced weight loss compared to control mice and to a lesser extent to mice receiving L. paracasei without the CPB formulation. Numerous studies demonstrated a significant effect of probiotic efficacy using a DSSinduced colitis model in mice 4,[36][37][38][39][40][41] . For instance, encapsulated L. rhamnosus GG exerted a reduced level of DSS-induced colonic injury compared to non-encapsulated L. rhamnosus GG 4 . ...
In this study, we show that calcium pectinate beads (CPB) allow the formation of 20 µm spherical microcolonies of the probiotic bacteria Lacticaseibacillus paracasei (formerly designated as Lactobacillus paracasei ) ATCC334 with a high cell density, reaching more than 10 log (CFU/g). The bacteria within these microcolonies are well structured and adhere to a three-dimensional network made of calcium-pectinate through the synthesis of extracellular polymeric substances (EPS) and thus display a biofilm-like phenotype, an attractive property for their use as probiotics. During bacterial development in the CPB, a coalescence phenomenon arises between neighboring microcolonies accompanied by their peripheral spatialization within the bead. Moreover, the cells of L. paracasei ATCC334 encased in these pectinate beads exhibit increased resistance to acidic stress (pH 1.5), osmotic stress (4.5 M NaCl), the freeze-drying process and combined stresses, simulating the harsh conditions encountered in the gastrointestinal (GI) tract. In vivo, the oral administration of CPB-formulated L. paracasei ATCC334 in mice demonstrated that biofilm-like microcolonies are successfully released from the CPB matrix in the colonic environment. In addition, these CPB-formulated probiotic bacteria display the ability to reduce the severity of a DSS-induced colitis mouse model, with a decrease in colonic mucosal injuries, less inflammation, and reduced weight loss compared to DSS control mice. To conclude, this work paves the way for a new form of probiotic administration in the form of biofilm-like microcolonies with enhanced functionalities.
... On the other hand, other well-characterized immunobiotic strains including L. acidophilus NCFM (14) or L. rhamnosus GG (15) did not attenuate symptoms. Furthermore, some probiotics such as L. crispatus M206119 have been found to aggravate dextran sulfate sodium (DSS)-induced colitis in mice (16). These findings highlight the importance of accurately evaluating each immunobiotic candidate to be used in IBD. ...
Immunobiotics have emerged as a promising intervention to alleviate intestinal damage in inflammatory bowel disease (IBD). However, the beneficial properties of immunobiotics are strain dependent and, therefore, each strain has to be evaluated in order to demonstrate its potential application in IBD. Our previous in vitro and in vivo studies demonstrated that Lactobacillus jensenii TL2937 attenuates gut acute inflammatory response triggered by Toll-like receptor 4 activation. However, its effect on colitis has not been evaluated before. In this work, we studied whether the TL2937 strain was able to protect against the development of colitis in a dextran sodium sulfate (DSS)-induced mouse model and we delved into the mechanisms of action by evaluating the effect of the immunobiotic bacteria on the transcriptomic response of DSS-challenged intestinal epithelial cells. L. jensenii TL2937 was administered to adult BALB/c mice before the induction of colitis by the administration of DSS. Colitis and the associated inflammatory response were evaluated for 14 days. Mice fed with L. jensenii TL2937 had lower disease activity index and alterations of colon length when compared to control mice. Reduced myeloperoxidase activity, lower production of pro-inflammatory (TNF-α, IL-1, CXCL1, MCP-1, IL-15, and IL-17), and higher levels of immunoregulatory (IL-10 and IL-27) cytokines were found in the colon of TL2937-treated mice. In addition, the treatment of porcine intestinal epithelial (PIE) cells with L. jensenii TL2937 before the challenge with DSS differentially regulated the activation of the JNK pathway, leading to an increase in epithelial cell integrity and to a differential immunotranscriptomic response. TL2937-treated PIE cells had a significant reduction in the expression of inflammatory cytokines (TNF-α, IL-1α, IL-1β, IL-6, IL-15), chemokines (CCL2, CCL4, CCL8, CXCL4, CXCL5, CXCL9, CXCL10), adhesion molecules (SELE, SELL, EPCAM), and other immune factors (NCF1, NCF2, NOS2, SAA2) when compared to control cells after the challenge with DSS. The findings of this work indicate that (a) L. jensenii TL2937 is able to alleviate DSS-induced colitis suggesting a potential novel application for this immunobiotic strain, (b) the modulation of the transcriptomic response of intestinal epithelial cells would play a key role in the beneficial effects of the TL2937 strain on colitis, and (c) the in vitro PIE cell immunoassay system could be of value for the screening and selection of new immunobiotic strains for their application in IBD.
... Yi Cui et al. found that compared with the control group, the experimental group of L. fermentum CCTCC M206110 yielded significantly reduced histological and disease activity index (DAI) scores, improved survival rate, and alleviated UC in BALB/c mice with dextran sulfate sodium (DSS)-induced UC (Cui et al., 2016). Elena Zagato et al. found that the fermentative metabolites of Lactobacillus paracasei CBA L74 can alleviate UC disorders by inhibiting the immune response, such as the release of pro-inflammatory cytokines (Zagato et al., 2014). ...
... Yi Cui et al. found that intragastric administration of L. crispatus CCTCC M206119 to mice with UC induced by 5% DSS aggravated the condition. The histological score and the mortality rate in the experimental group were significantly higher than that of the control group (Cui et al., 2016). Bifidobacterium CMS-H004 and CH22 strains also had similar negative effects on the severity of UC induced by DSS, while the two strains did not cause inflammation in healthy rats (Chen, 2007). ...
Controversial results were yielded in some studies on the effects of Lactobacillus on ulcerative colitis (UC). In this work, we evaluated the effect of live or heat-killed Lactobacillus plantarum Zhang-LL cells and cell supernatants on dextran sulfate sodium (DSS)-induced UC in rats. The tested subjects were gavaged to rats for 7 days, and then 5% was used DSS repeatedly to induce chronic UC. The results of a 42-day experiment, indicated consistently and significantly that Both living and heat-killed cells of L. plantarum Zhang-LL could down-regulate content of pro-inflammatory cytokines (TNF-α, INF-γ) in serum, reduce intestinal permeability by increasing the expression of tight junction proteins zo-1, claudin-1 and occludin in colon tissue, and maintain intestinal microbiota stability by increasing intestinal microbiota diversity to alleviate the symptoms of chronic UC in rats with 100% survival rate. Of concern is, the heat-killed cells of L. plantarum Zhang-LL were more effective against DSS-induced chronic UC in rats.
... interestingly, the strains which had better probiotic effect during the our previous research showed more inhibitory effect on the colon length reduction of colitis mice. These results are in accordance with Cui et al. [16] who demonstrated that L. plantarum and Lactobacillus fermentum had significantly reduced shortening of the colon length. Chen et al. [17] have reported that L. fermentum had the ability to effectively alleviate the colon length. ...
Inflammatory bowel disease (IBD) comprises two major illnesses: Crohn's disease (CD) and ulcerative colitis (UC). Dextran sulfate sodium (DSS) mouse colitis model has been used in understanding the mechanism of IBD. This study was conducted to examine selected Lactobacillus spp. as potential IBD treatment in the DSS-induced animal model. Balb/c mice were used and colitis was induced by adding 5% dextran sodium sulfate into the drinking water for 8 days. Colon length, disease activity index (DAI) and histological analysis were measured as markers of inflammation in DSS colitis mice. The majority of the Lactobacillus species significantly prevented the shortening of the colon length compared with the DSS group. The DAI scores of mice were significantly reduced following usage of four Lactobacillus strains included: Lactobacillus plantarum 03 and 06, Lactobacillus brevis 02 and Lactobacillus rhamnosus 01. The histological analysis exhibited that oral administration of Lactobacillus strains had therapeutic effects on mice colitis. L. plantarum and L. brevis showed better therapeutic effect against DSS-induced acute colitis mice. The probiotic activities of these three isolates indicated that the probiotic effects were strain specific and none of these useful bacteria could exhibit all of the valued probiotic properties simultaneously.
... Similarly, herein, the Firmicutes phylum was decreased in a significant way (p < 0.05) under DSS effect and was not affected by the addition of GSM into the diet in a significant way. Clostridium, Roseburia, Acidaminococcus, and Escherichia are often cited as the genera usually found in abundance in irritable bowel diseases (48)(49)(50)(51)(52) and DSS treatment, while Lactobacillus, Prevotella, and Faecalibacterium are cited as negatively impacted or inversely correlated with the severity of the disease (33,(53)(54)(55)(56). Indeed, in our work, Roseburia, Megasphera, and CF231 increased significantly under the DSS presence while Lactobacillus registered a significant decrease overall. ...
Microbiota affects host health and plays an important role in dysbiosis. The study examined the effect of diet including grape seed meal (GSM) with its mixture of bioactive compounds on the large intestine microbiota and short-chain fatty acid synthesis in weaned piglets treated with dextran sodium sulfate (DSS) as a model for inflammatory bowel diseases. Twenty-two piglets were included in four experimental groups based on their diet: control, DSS (1 g/kg/b.w.+control diet), GSM (8% grape seed meal inclusion in control diet), and DSS+GSM (1 g/kg/b.w., 8% grape seed meal in control diet). After 30 days, the colon content was isolated and used for microbiota sequencing on an Illumina MiSeq platform. QIIME 1.9.1 pipeline was used to process the raw sequences. Both GSM and DSS alone and in combination affected the diversity indices and Firmicutes:Bacteroidetes ratio, with significantly higher values in the DSS-afflicted piglets for Proteobacteria phylum, Roseburia, Megasphera and CF231 genus, and lower values for Lactobacillus. GSM with high-fiber, polyphenol and polyunsaturated fatty acid (PUFA) content increased the production of butyrate and isobutyrate, stimulated the growth of beneficial genera like Prevotella and Megasphaera, while countering the relative abundance of Roseburia, reducing it to half of the DSS value and contributing to the management of the DSS effects.
