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Inonotus obliquus, a parasite of the birch tree, is also a well-known medicinal mushroom. Its sterile conk is highly sought for its bioactive compounds such as phenols, polysaccharides, triterpenoids, and steroids. It was traditionally used to treat various gastrointestinal diseases, viral and parasitic infections, to counteract the progression of...
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Sanghuangporus baumii is a forest pathogenic fungus and also a medicine mushroom. In the wild, S. baumii parasitize on living host plants and there have been no reports of cultivating fruiting bodies under artificial conditions. In this study, we identified and isolated a S. baumii strain, and successfully cultivated the fruiting bodies on sawdust...
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... We have characterized the 4-hydroxyisoleucine by the biophysical methods, extracting the isolated pure compound from the silica gel and subjected to structural elucidation by the HPLC, LCMS and NMR, and our results are in agreement with many researchers [37][38][39][40]. In recent years, there are several reports on the stimulation of bioactive compound from gamma irradiated medicinal plants [14,41], mushrooms [42], agro-industrial residues [43]. This study also in agreement with the recent studies, for the first time fenugreek extracts irradiated with gamma radiation of 20 Gy found to be very effective by providing enhanced 4-hydroxyisoleucine bioactive compound which has strong anti-blast property and can protect rice plants against blast disease. ...
In the current study, the influence of gamma radiation for the enhanced activity of 4-hydroxyisoluceine (4-HIL) that posses an in vitro inhibition of blast pathogen, thereby protecting rice plants was thoroughly investigated. It was found that methanolic crude extract of 20 Gy gamma irradiated extract provided remarkable saponin content (7.15 g 100 g⁻¹) compared to non-irradiated extracts. When irradiated and non-irradiated crude extracts on separation by thin layer chromatography (TLC) showed an Rf value of 0.61 that matched with the Rf value of the standard 4-HIL compound. Further, the TLC partial purified compound (10 μl) upon ejected to high performance liquid chromatography (HPLC) produced a single peak at a retention time of 5.1 min. Moreover, a fair to good anti-blast properties was noticed in all the concentrations of the purified 4-HIL compound. Analyses of anti-blast compound using liquid chromatography-mass (LCMS) spectrometry and 2-D nuclear magnetic resonance spectroscopy (NMR) detected the presence of 4-HIL at m/z value of 146 and CD3OD resonating at 3.86 ppm respectively. Lastly, under greenhouse conditions, primed plants with 4-HIL compound at 150 μg mL⁻¹ showed a significant disease protection of 77% over the control plants. Overall, this study witnessed an enhanced anti-blast secondary compound from fenugreek seeds irradiated with gamma rays which can be used as safe agrochemicals for plant protection, particularly for rice production.
... Other associated booming methodologies were the acute gamma ionising radiation at sublethal doses of the mycelium mass. This induced artificial abiotic stress procedure promoted the synthesis of bioactive metabolites with enhanced antioxidant properties (Petre et al., 2021). ...
The traditional and growing importance of mushrooms due to their rich composition in nutritive and bioactive compounds converts the whole feedstock and their fractions into versatile attractive ingredients for food and nutraceuticals. The processing conditions are critically relevant to selectively recover high‐valuable compounds in a sustainable way. This short review, covering scientific papers published in the last 2 years, offers an updated vision of the study and applications of edible mushroom bioactive compounds, covering aspects in relation to the novelties in the cultivation, isolation, identification, characterisation of chemical and biological properties, extraction technologies and purification, as well as food applications, particularly in novel foods.
