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The phenotypes (A), ratio of root/shoot (B) and dry weight (C, D) of the transgenic lines as exposed to phosphorus treatments. Different letters within the same panel and same plant tissue indicated significant difference (p < 0.05)
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Previously, we cloned the full sequence of masson pine (Pinus massoniana) phosphate transporter gene (PmPT1) from a phosphorus (Pi) deficiency tolerant strain. To further verify whether PmPT1 presumably function in angiosperms, i.e. tobacco, as well as to generate the new germplasm with high tolerance to Pi deficiency, currently, this gene was tran...
Citations
... Biotechnologists and breeders have been consistently working on deciphering the molecular and physiological determinants of plant nutrition under optimal as well under stress-prone conditions. Several stress-responsive genes (including mineral transporters, aquaporins, and vacuole transporters) that regulate nutrient uptake, accumulation, and their metabolism in plants have been identified and manipulated by genetic engineering approaches to produce transgenics with improved nutritive values (Bao et al., 2009(Bao et al., , 2015Zhang, Hong, et al., 2020). Biofortification using molecular plant breeding approaches has also been undertaken to address the issues of malnutrition and ensure nutrition and food security (Calayugan et al., 2020). ...
... The overexpression of codA gene in tomato significantly enhanced Pi uptake, translocation capacity, and aided in the maintenance of Pi homeostasis and resulted in high shoot sucrose levels, increased photosynthesis, root development, and enhanced crop yield . Similarly, overexpressing PmPT1, a phosphate transporter increases P accumulation in the roots and shoots, dry weight, enhances chlorophyll levels, soluble sugar and protein contents, and activity of antioxidant enzymes along with decreased MDA levels (Zhang, Hong, et al., 2020). ...
Plant nutrition is an important aspect that contributes significantly to sustainable agriculture, whereas minerals enrichment in edible source implies global human health; hence, both strategies need to be bridged to ensure “One Health” strategies. Abiotic stress‐induced nutritional imbalance impairs plant growth. In this context, we discuss the molecular mechanisms related to the readjustment of nutrient pools for sustained plant growth under harsh conditions, and channeling the minerals to edible source (seeds) to address future nutritional security. This review particularly highlights interventions on (i) the physiological and molecular responses of mineral nutrients in crop plants under stressful environments; (ii) the deployment of breeding and biotechnological strategies for the optimization of nutrient acquisition, their transport, and distribution in plants under changing environments. Furthermore, the present review also infers the recent advancements in breeding and biotechnology‐based biofortification approaches for nutrient enhancement in crop plants to optimize yield and grain mineral concentrations under control and stress‐prone environments to address food and nutritional security.
... This suggestion is supported by the higher accumulation of both H 2 O 2 and ·O 2 − in R--roots than in R++, R+, and R-roots (Fig. 10B, C, G, H). These findings agree with previous reports that Pi starvation increases ROS production and activity of ROS-scavenging enzymes (Shin et al., 2005;Zhang et al., 2020). In addition, there was no significant difference in trypan blue staining among R++, R+, and R-, but staining of R--was deeper than that of R++, R+, and R-, which indicated that ROS accumulation was caused by Pi starvation and not by apoptosis ( Supplementary Fig. S3). ...
Due to the non-uniform distribution of inorganic phosphate (Pi) in the soil, plants modify their root architecture to improve acquisition of this nutrient. In this study, a split-root system was employed to assess the nature of local and systemic signals that modulate root architecture of Brassica napus grown with non-uniform Pi availability. Lateral root (LR) growth was regulated systemically by non-uniform Pi distribution, by increasing the density of the second-order LR (2˚LR) in compartments with luxury Pi supply but decreasing the 2˚LR density in compartments with low Pi availability. Transcriptomic profiling identified groups of genes regulated, both locally and systemically, by Pi starvation. The number of systemically induced genes was greater than the number that was locally induced and included genes related to abscisic acid (ABA) and jasmonic acid (JA) signalling pathways, reactive oxygen species (ROS) metabolism, sucrose, and starch metabolism. Physiological studies confirmed the involvement of ABA, JA, sugars, and ROS in the systemic Pi starvation response. The data reported reveal the mechanistic basis of local and systemic responses of B. napus to Pi starvation and provide new insights into the molecular and physiological basis of root plasticity.
