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The inhibition rate of the various concentrations of epirubicin (a) and petroleum ether extracts of Curcuma zedoaria (b) were determined by CCK8 assays. The values were reported as %. (c) The inhibition rate of different days was determined by CCK8 assay as described compared with vehicle. Values are average of triplicate experiment and are represented as mean ± SD.
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Objective. To evaluate the effect of petroleum ether extracts of Curcuma zedoaria on the proliferation of human triple negative breast cancer cell line MDA-MB-231. Methods. The reagents were isolated from Curcuma zedoaria by petroleum ether fraction. It was assayed by CCK8 for MDA-MB-231 cellular viability with various concentrations and days, cell...
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Background: No standard chemotherapy is used as neoadjuvant therapy in triple negative breast cancer (TNBC). This study has compared carboplatin plus paclitaxel with commonly used epirubicin plus paclitaxel as neoadjuvant chemotherapy (NAC) in TNBC.
Results: 91 patients with a median age of 47 years (PC 47 patients, EP 44 patients) were enrolled. 6...
Citations
... It has been proved from the western blotting and other analytical techniques that, cytotoxic activity was due to arrest of cell cycle at G0/G1 phase. It also results in elevation in amount of expression proteins like Ecadherin and it lower the level of SDF-1, CCR7 and CCR4 mRNA [44] . Previous studies reported data lined the role of Curcumin alone to counter act the breast cancer. ...
Cancer recognized as a serious disorder among population worldwide. Breast cancer has been found common in women. Breast cancer diagnosed patients increasing with passage of time, which bringing the attention of researcher to give more potential strategies to irradiate this ailment from the society. Natural remedies from medicinal plants are well acknowledged from primitive time of decade. Modern and advanced analytical tools successfully overcome the burden of this disease by incorporating raw formulations into suitable dosage forms and their efficacy can be determined through experimental and clinical studies. There are numerous medicinal plants present having pharmacological potential to decreases the breast cancer cell viability by involving various mechanisms and significantly overcome the global burden of this disorder. Our presented study motivated from the burden of breast cancer among people and its treatment from natural sources. However, it is much needed to understand etiology of the disease and its associated causes. We also demonstrated the treatment strategies originated from natural sources that conquer the spectrum of this disorder. There are numerous types of natural products that has preventive and curative role in the management of breast cancer. So, accurate method and terminology required to elevating the demand of health care system from natural sources can overcome breast cancer.
... Moreover, consuming a diet rich in micronutrients such as fiber, minerals, vitamins and phytonutrients has been reported to reduce the risk of BC and to demonstrated preventative anti-cancer activity (26). Many phytochemical compounds including phenolic compounds (such as, coumarins, flavonoids, lignans, phenolic acids, quinones, stilbenes and tannins), nitrogen compounds (such as alkaloids, amines and betalains), vitamins (such as A, C, D and E) and terpenes (including carotenoids) derived from certain herbs in the TTFM recipe have been reported to have the cytotoxic effects against certain cancer cells, such as non-small cell lung cancer (13,14), cervical cancer (27), colon cancer (28), gastric cancer (29), leukemic cancer (15,24) and breast cancer (22,23,25). Furthermore, a previous in vitro study reported that a variety of phytochemical substances plays a crucial part in inflammation in breast cancer (30). ...
Breast cancer is a leading cause of cancer-related deaths worldwide. Moreover, standard treatments are limited, so new alternative treatments are required. Thai traditional formulary medicine (TTFM) utilizes certain herbs to treat different diseases due to their dominant properties including anti-fungal, anti-bacterial, antigenotoxic, anti-inflammatory and anti-cancer actions. However, very little is known about the anti-cancer properties of TTFM against breast cancer cells and the underlying molecular mechanism has not been elucidated. Therefore, the present study, evaluated the metabolite profiles of TTFM extracts, the anti-cancer activities of TTFM extracts, their effects on the apoptosis pathway and associated gene expression profiles. Liquid chromatography with tandem mass spectroscopy analysis identified a total of 226 compounds within the TTFM extracts. Several of these compounds have been previously shown to have an anti-cancer effect in certain cancer types. The MTT results demonstrated that the TTFM extracts significantly reduced the cell viability of the breast cancer 4T1 and MDA-MB-231 cell lines. Moreover, an apoptosis assay, demonstrated that the TTFM extracts significantly increased the proportion of apoptotic cells. Furthermore, the RNA-sequencing results demonstrated that 25 known genes were affected by TTFM treatment in 4T1 cells. TTFM treatment significantly up-regulated Slc5a8 and Arhgap9 expression compared with untreated cells. Moreover, Cybb, and Bach2os were significantly downregulated after TTFM treatment compared with untreated cells. Reverse transcription-quantitative PCR demonstrated that TTFM extract treatment significantly increased Slc5a8 and Arhgap9 mRNA expression levels and significantly decreased Cybb mRNA expression levels. Moreover, the mRNA expression levels of Bax and Casp9 were significantly increased after TTFM treatment in 4T1 cells compared with EpH4-Ev cells. These findings indicated anti-breast cancer activity via induction of the apoptotic process. However, further experiments are required to elucidate how TTFM specifically regulates genes and proteins. This study supports the potential usage of TTFM extracts for the development of anti-cancer drugs.
... The mechanism of cell death that is exerted by some compounds of Curcuma zedoaria and the cytotoxic components of Curcuma zedoaria is reported (Matthes et al., 1980;Shiobara et al., 1985;Matsuda et al., 1998;Jang et al., 2004;Park et al., 2005;Oh et al., 2007;Lobo et al., 2009;Ahmed Hamdi et al., 2010;Widjastuti and Andriani, 2010;Lakshmi et al., 2011;Lu et al., 2012;Kimura et al., 2013;Rahman et al., 2013). Petroleum ether extracts of Curcuma zedoaria inhibited MDA-MB-231 cells (Gao et al., 2014). It's shown that Curcuma zedoaria can possess several compounds which have antiproliferative effect on four cancer cell lines, including MCF-7, PC-3, Ca Ski, and HT-29. ...
Skin cancer is one of the most prevalent cancers worldwide. Some limited therapy methods, including surgery, radiation therapy, and chemotherapy, are performed for squamous cell carcinoma, basal cell carcinoma, and melanoma, as well-known skin cancers. The current treatment methods have a relatively low success rate and may leave different side effects during and after therapy. Therefore, finding alternative methods without side effects to treat skin cancer has always been interesting to oncology researchers. Thus, nutraceuticals and herbal medicines for skin cancer treatment have become particularly important in recent years. In this regard, the main classes of nutraceutical compounds with powerful anticancer potential discussed in this book chapter are included phenolic acids, flavonoids, carotenoids, vitamins, and terpenes.
... The ethanolic extract was obtained according to the method reported by Gao et al. [21,22], with minor modifications. Briefly, 0.5g of C. caesia powder was dissolved in 15mL of 50% ethanol (1:1, v/v) and subjected to 30min in an ultrasonic bath (model 2800 A, Unique®, 40kHz, 154W, São Paulo, Brazil) at a frequency of 40kHz and a potency of 107W at room temperature. ...
... The main functions of Xiaoji recipe is to eliminate the heat and dampness and promote the blood circulation and can be used in the antitumor therapy for various cancers. Curcuma zedoaria (Zingiberaceae) is reported to inhibit the development of gastric carcinoma, breast cancer as well as liver cancer [11][12][13][14]. Polygonum cuspidatum is used for the therapy of multiple diseases including hypertension, diabetes, and atherosclerosis [15][16][17], and its extracts have been reported with anticancer effects in lung cancer, osteosarcoma, and breast cancer [18][19][20]. ...
Traditional Chinese medicine (TCM) is applied in the anticancer adjuvant therapy of various malignancies and pancreatic cancer included. Xiaoji recipe consists several TCM materials with anticancer activities. In our work, we intended to analyze the molecular targets as well as the underlying mechanisms of Xiaoji recipe against pancreatic cancer. A total of 32 active components and 522 potential targets of Xiaoji recipe were selected using the TCMSP and SwissTargetPrediction databases. The potential target gene prediction in pancreatic cancer was performed using OMIM, Disgenet, and Genecards databases, and totally, 998 target genes were obtained. The component-disease network was constructed using the Cytoscape software, and 116 shared targets of pancreatic cancer and Xiaoji recipe were screened out. As shown in the protein–protein interaction (PPI) network, the top 20 hub genes such as TP53, HRAS, AKT1, VEGFA, STAT3, EGFR, and SRC were further selected by degree. GO and KEGG functional enrichment analysis revealed that Xiaoji recipe may affect pancreatic cancer progression by targeting the PI3K/AKT and MAPK signaling pathways. Moreover, we performed in vitro assays to explore the effect of Xiaoji recipe on pancreatic cancer cells. The results revealed that Xiaoji recipe suppressed the viability and migration and promoted the apoptosis of pancreatic cancer cells via the inactivation of PI3K/AKT, MAPK, and STAT3 pathways. The findings of our study suggested the potential of Xiaoji recipe in the targeting therapy of pancreatic cancer.
... Tingkat ekspresi protein E-cadherin dan E-cadherin mRNA meningkat secara signifikan, sedangkan protein SDF-1, CCR7, dan CXCR4 mRNA 199 mengalami penurunan setelah di inkubasi dengan ekstrak petroleum eter kunir putih pada konsentrasi 300 µg/mL dari kontrol (<0,05). Perbedaannya adalah tingkat ekspresi mRNA protein CXCR4 lebih tinggi daripada pembawa(14). Pada penelitian lain juga mempelajari efek dari ekstrak kunir putih pada sel darah tepi untuk perkembangan tumor di C57Bl/6J pada tikus yang disuntik dengan sel melanoma murine B16F10. ...
AbstrakKunir putih (Curcuma zedoaria Rosc.) adalah tanaman herbal yang memiliki populasi cukup banyak di Indonesia. Kunir putih telah lama digunakan karena berdasarkan data empiris memiliki aktivitas sebagai antioksidan, anti bakteri, anti inflamasi dan anti kanker. Hal ini mengindikasikan kunir putih (Curcuma zedoaria Rosc.) memiliki bahan aktif atau metabolit sekunder seperti curcuminol yang memberikan aktivitas tersebut. Penggunaan kunir putih pada kalangan masyarakat terbatas pada obat herbal dan jamu dengan pengolahan standar sehingga saat ini banyak dikembangakan ulasan mengenai formulasi dan inovasi dari kunir putih sehingga lebih memberikan aktivitas terapi. Pada review artikel ini akan diulas secara lengkap mengenai kunir putih dalam hal formulasi yang telah dikembangkan, kandungan kimia dan aktivitas biologi yang telah teruji pada kunir putih. Kata kunci: Aktivitas biologi, Formulasi, Kunir putih
... Aromatic turmenone or 6S-2-methyl-6-(4-methylphenyl) hept-2-en-4-one is an antitumor bisabolane sesquiterpene [45], that has been extracted from C. longa, C. amada and C. wenyujin [46][47][48]. ...
Cancer is a multifaceted disease characterized by deregulated epigenetic, genetic and metabolic signals which affect cellular metabolism and apoptosis. Breast cancer is regarded to be the most common malignancy in the women worldwide. The side effects of chemo-drugs such as non-selectivity, toxicity and resistance urge scientists to find more potent and safer drugs. Natural products from plants provide an extensive array of chemical scaffolds with biosafety profiles, and safer health effects. Curcuma, a genus of family Zingiberaceae, comprises of about 110 species natively distributed as well as cultivated in South Asia, China, Australia, Sri Lanka, West Indies, and Peru. This plant is used as a remarkable pharmacological remedy to prevent and cure various pathological disorders including cancer. The chemical constituents of this plant, terpenoids and betaketones, specifically act as the anti-breast cancer agents. Curcuma, the marvel of nature, can be regarded as panacea due to its versatile molecular targets and spacious therapeutic window. Various investigations on the essential oils and dry rhizome conclude that the chemical constituents of this plant e.g., curcumin, germacrone, furanodienone, bisdethoxycurcumin, demethoxycurcumin, curcumol, and aromatic turmerone are responsible for its anti-breast cancer potential. These compounds, either as single compound or in combinations with other drugs, are known to arrest cell cycle and induce apoptosis in breast cancer cells by modulating various signaling cascades including NF-κB, STAT3, PI3k/Akt/mTOR and MAPK. This book chapter intends to comprehend the biological and pharmacological mode of action of Curcuma-derived anti-breast cancer compounds in order to update the researchers and scientific community about the pharmaceutical potential of plants belonging to this genus.
... It also exerts anti-thrombosis, anti-platelet aggregation, anti-atherosclerosis, blood lipid regulation effects (11). Moreover, the petroleum ether extracts of Curcuma zedoaria (PECZ) have been shown to inhibit the proliferation of MDA-MB-231 cells (12). However, the mechanism of PECZ and whether PECZ can reverse docetaxel tolerance in TNBC remains unclear. ...
... It is well known that Curcuma zedoaria is a common chemotherapy drug for cancer-related diseases, such as cervical, colon, and especially breast cancers (18). It has been shown that PECZ possesses the strongest inhibitory effect on MDA-MB-231 cells compared to other extracts (12). Consistent with these results, we also found that the proliferation of MDA-MB-231/docetaxel cells was markedly inhibited after pre-treatment with PECZ. ...
Background:
Triple-negative breast cancer (TNBC) is characterized by its aggressiveness and poor prognosis. Docetaxel is the common chemotherapeutic drug used in the treatment of TNBC. However, resistance to docetaxel has limited the effectiveness of TNBC treatment. Petroleum ether extracts of Curcuma zedoaria (PECZ) can inhibit the proliferation of MDA-MB-231 cells. However, the effect of PECZ on docetaxel resistance is not clear.
Methods:
A docetaxel-resistant MDA-MB-231 (MDA-MB-231/docetaxel) cell line was established, and Cell Counting Kit-8 (CCK-8), quantitative real-time PCR (qRT-PCR), and western blotting assays were used to evaluate the effect of docetaxel resistance in MDA-MB-231 cells. Next, CCK-8 was also performed to detect the effect of docetaxel or the combination treatment of docetaxel and PECZ on the proliferation of MDA-MB-231/docetaxel cells. Thereafter, MDA-MB-231/docetaxel cells were subcutaneously injected into nude mice to induce a TNBC xenograft model, and the mice were divided into a model group, docetaxel group, PECZ group, and combination of docetaxel and PECZ group. Subsequently, hematoxylin and eosin (HE) staining, immunohistochemical, qRT-PCR, and western blotting were used to estimate the effect of pre-treatment with PECZ on docetaxel tolerance reversal.
Results:
PECZ significantly inhibited the expression of pregnane X receptor (PXR), multidrug resistance 1 (MDR1), breast cancer resistance protein (BCRP), and cytochrome P-450 (CYP3A4) in MDA-MB-231/docetaxel cells. Only higher concentrations of docetaxel could inhibit the viability of MDA-MB-231/docetaxel cells. When pre-treated with PECZ, lower concentrations of docetaxel could significantly inhibit cell viability. Meanwhile, combination treatment also reduced the tumor volume, ameliorated the pathological change of tumor tissues, and down-regulated the expressions of PXR, MDR1, BCRP, and CYP3A4 (according to HE staining, immunohistochemical, qRT-PCR and western blotting results in vivo).
Conclusions:
Our research showed that PECZ reversed docetaxel resistance in TNBC by PXR both in vitro and in vivo, which provides the basis for further investigations into the potential therapeutic impact of docetaxel resistance in TNBC.
... J. of Life Sciences, Special issue, A13; December, 2019 cells) in a dose dependant manner and inhibits cell proliferation and induced cell apoptosis . Petroleum ether extracts of C. zedoaria inhibits the proliferation of human triple negative breast cancer cell line MDA-MB-231 (Gao et al., 2014). Active compound isocurcumenol isolated from the rhizomes of C. zedoaria inhibits the proliferation of cancer cells without inducing the significant toxicity to normal cells and in vivo doses of 37.7 mg/kg body weight significantly reduce the ascetic tumor in DLA-challenged mice and increase the life span compare to untreated control mice (Lakshmi, 2011). ...
Phytochemical investigation of methanol extracts of Curcuma zedoaria (Christm) Roscoe rhizome (Zingiberaceae) yielded 9 major phytoconstituents by using High Resolution Liquid Chromatography - Mass Spectroscopy (HRLC-MS) technique. The mass spectra of compounds found in extract was matched with Metlin database library, results confirmed the presence of therapeutically potent chemical compounds. Metabolites analysis by ESI-Q-TOF-MS revealed presence of 9 major abundant compounds namely Glycopyrrolate, Cucurbitacin I, Flurandrenolide, 26,26,26,27,27,27-hexafluoro-1alpha, 24-dihydroxyvitaminD3 / 26,26,26,27,27,27 - hexafluoro-1alpha, Proto porphyrinogen IX, Phenylbutazone glucuronide, Methyl Gamboginate, Propofol, Ibuprofen. This report is the first of its kind to analyze chemical constituents of Curcuma zedoaria using HR-MS. In addition to this, the results of HRMS profile can be used as pharmacognostical tool for identification of plant. Key words: Curcuma zedoaria, Phytochemical, HRLC-MS, Cucurbitacin I, Flurandrenolide
... C. rhizoma is widely used in the therapy of UL but absolutely prohibited for the pregnant women in clinics (15)(16)(17). It possesses anti-inflammatory and anti-tumor activities and responds to multiple regulation of signaling pathways for ECM deposition (18), cell cycle arrest (19), and UL process (20). Moreover, TCM has long established the concise and synergistic effects of C. rhizoma and S. rhizoma, and, as a consequence, a compound with both was developed for UL therapies (21). ...
... The essential oil of C. rhizoma presented anti-angiogenic activity in vitro and in vivo, resulting in the suppression of tumor growth and metastasis, by inhibiting the phosphorylation of ERK1/2 and AKT/NF-kB signaling pathways, and enhancing the phosphorylation of JNK1/2 and p38 (16)(17)(18). Petroleum ether extracts of C. rhizoma produce a significant G0/G1 cell cycle arrest at the concentration of 300 mg/mL (19). Curcumenol, a sesquiterpene isolated from C. rhizoma, possess anti-inflammatory and anti-tumor activities by inhibiting Akt-dependent NF-kB and p38-MAPK signaling (20). ...
The aim of this study was to elucidate the concise effects of a traditional herb pair, Curcumae rhizoma-Sparganii rhizoma (CRSR), on uterine leiomyoma (UL) by analyzing transcriptional profiling. The UL rat model was made by intramuscular injection of progesterone and gavage administration of diethylstilbestrol. From 11 weeks of the establishment of the model, rats of the UL+CRSR group were gavaged daily with CRSR (6.67 g/kg). The serum concentrations of progesterone (P) and estradiol (E2) were determined by radioimmunoassay, the uterine index was measured by caliper measurement, and the pathological status was observed by hematoxylin and eosin stain. Gene expression profiling was checked by NimbleGen Rat Gene Expression Microarrays. The results indicated that the uterine mass of UL+CRSR rats was significantly shrunk and serum P and E2 levels significantly reduced compared to UL animals and nearly to the level of normal rats. Results of microarrays displayed the extensive inhibition of CRSR upon the expression of proliferation and deposition of extracellular matrix (ECM)-related genes, and significantly regulated a wide range of metabolism disorders. Furthermore, CRSR extensively regulated key pathways of the UL process, such as MAPK, PPAR, Notch, and TGF-β/Smad. Regulation of the crucial pathways for the UL process and ECM metabolism may be the underlying mechanisms of CRSR treatment. Further studies will provide clear clues for effectively treating UL with CRSR.
