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The influence of different concentrations of bisol 2 and baitan on callus morphology and the pattern of fungal mycelium development in combined cultures of wheat calluses and the bunt pathogen Tilletia caries; 20 days after pathogen inoculation.
Source publication
A study was made of the influence of bisol 2 and baitan compounds on morphogenesis and defence response of wheat calluse cells infected with bunt agent in associated with oxalate oxidase activation. After introduction of bisol and baitan into cultivation medium, dense area with meristema zones, germs of shoots and rhizoids appeared on non-morphogen...
Citations
The influence of salicylic (SA) and jasmonic (JA) acids as signaling systems mediators on the generation of H2O2 and expression of genes encoding protective proteins was studied in the leaves of wheat Triticum aestivum L. upon infection with the pathogen of septoriosis Septoria nodorum Berk. It was found that presowing treatment of seeds with SA and JA decreased the development of the fungus on the leaves of wheat and had a stimulating effect on the production of H2O2 in the area of infection. An increased expression of genes encoding oxalate oxidase AJ556991.1 and anionic peroxidase TC 151917 was shown in infected tissues with the method of polymerase chain reaction.
We studied the effect of hydrogen peroxide on morphological characteristics and resistance of common wheat calluses ( Triticum aestivum L.) to Tilletia caries Tul. The induction of the defense response and morphogenesis in calluses depended on H2O2 concentration. A correlation was revealed between the elevated concentration of hydrogen peroxide in wheat calluses and high
activity of oxalate oxidase in the cell wall. Administration of H2O2 into the callus culture medium was followed by rhizogenesis, induced the formation of dense regions, and inhibited fungal
growth on calluses. Hydrogen peroxide at high concentrations was less potent in inhibiting the growth of fungi. A relationship
was found between oxalate oxidase activity, H2O2 concentration, and morphogenetic and defense responses of calluses induced by exogenous hydrogen peroxide. These data suggest
that the induction of H2O2 generation is one of the approaches to increase callus resistance.
