The compared results using our proposed probability map-and local entropy-based methods. Each row shows four slice images from a wild-type embryo. Red contour denotes the detected nuclear boundary by our method, and green contour denotes the boundary by the local entropy-based method  

The compared results using our proposed probability map-and local entropy-based methods. Each row shows four slice images from a wild-type embryo. Red contour denotes the detected nuclear boundary by our method, and green contour denotes the boundary by the local entropy-based method  

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Nuclear detection in embryos is an indispensable process for quantitative analysis of the development of multicellular organisms. Due to the overlap in the distribution of pixel intensity of nuclear and cytoplasmic regions and the large variation of pixel intensity even within the same type of cellular components in different embryos, it is difficu...