Figure 10 - uploaded by Swathi Nedunchezian
Content may be subject to copyright.
The cell viability evaluation by MTS assay in hADSCs were cultured with the photocured hybrid hydrogel of HG, HG+0.5% AFnSi, and HG+1% AFnSi. Only the HA was controlled. Error bar represents ± SD (n = 3); * p< 0.05 compared with HA group, respectively.
Source publication
Similar publications
The poor mechanical properties of chitosan physical hydrogels seriously hinder their application in the biomedical field. Inspired by the structure of cell tissues, a novel chitosan nanofiber (CSNF)/Hyaluronic acid (HA)/β-glycerophosphate disodium (β-GP) drug-loaded hydrogel was prepared by micro-dissolution and physical crosslinking. The hydrogel...
Citations
... In this study, we showed that overloading pressure in TMJ chondro- Light-crosslinked hydrogels, such as GelMA and HAMA, are widely employed as tissue engineering scaffolds. 39,40 In this study, we employed these hydrogels to mimic in vivo conditions. Safranin O We expanded our previous observations to the forced-mouth opening model in SD rats, a validated model known to induce TMJ cartilage degeneration and subchondral bone changes. ...
Excessive load on the temporomandibular joint (TMJ) is a significant factor in the development of TMJ osteoarthritis, contributing to cartilage degeneration. The specific mechanism through which excessive load induces TMJ osteoarthritis is not fully understood; however, mechanically‐activated (MA) ion channels play a crucial role. Among these channels, Piezo1 has been identified as a mediator of chondrocyte catabolic responses and is markedly increased in osteoarthritis. Our observations indicate that, under excessive load conditions, endoplasmic reticulum stress in chondrocytes results in apoptosis of the TMJ chondrocytes. Importantly, using the Piezo1 inhibitor GsMTx4 demonstrates its potential to alleviate this condition. Furthermore, Piezo1 mediates endoplasmic reticulum stress in chondrocytes by inducing calcium ion influx. Our research substantiates the role of Piezo1 as a pivotal ion channel in mediating chondrocyte overload. It elucidates the link between excessive load, cell apoptosis, and calcium ion influx through Piezo1. The findings underscore Piezo1 as a key player in the pathogenesis of TMJ osteoarthritis, shedding light on potential therapeutic interventions for this condition.
... Additionally, the mechanical rigidity of the hydrogel can have an impact on cellular activity and tissue development. In tissue engineering applications, the promotion of chondrocyte differentiation and functional cartilage tissue formation can be significantly influenced by the porosity and other properties of hydrogels [43,44]. SAED pattern of the nanocomposite at higher CQD concentration (0.1 %), demonstrates a ring pattern which is in stark contrast with the highly crystalline orientation of the nanocomposite at lower concentration of CQDs (0.05 %) ( Fig. 1h and i). ...
... The optical photo of the tissue-engineered bilayer osteochondral graft is shown in Fig. 3a. We have previously provided a detailed in vitro analysis of the ability of our biohydrogel system to promote chondrogenic differentiation of ADSCs in our published paper [32]. However, the ADSCs within this biohydrogel can exchange nutrients and metabolites through diffusion in the external physiological environment. ...
... After the as-received 3D-printed bioceramic greenbody is obtained, the residual water in the sample must be removed by drying [40,41]. Deformation and cracks of the sample can occur quite easily during the drying process with the traditional natural drying method [32,40]. In particular, layer delamination and cracking can frequently occur due to the internal stress generated when materials such as solvents, residues, and polymers emerge from the objects during the drying and debinding processes [41]. ...
Reconstruction of severe osteochondral defects in articular cartilage and subchondral trabecular bone remains a challenging problem. The well-integrated bilayer osteochondral graft design expects to be guided the chondrogenic and osteogenic differentiation for stem cells and provides a promising solution for osteochondral tissue repair in this study. The subchondral bone scaffold approach is based on the developed finer and denser 3D β-tricalcium phosphate (β-TCP) bioceramic scaffold process, which is made using a digital light processing (DLP) technology and the novel photocurable negative thermo-responsive (NTR) bioceramic slurry. Then, the concave-top disc sintered 3D-printed bioceramic incorporates the human adipose-derived stem cells (hADSCs) laden photo-cured hybrid biohydrogel (HG + 0.5AFnSi) comprised of hyaluronic acid methacryloyl (HAMA), gelatin methacryloyl (GelMA), and 0.5% (w/v) acrylate-functionalized nano-silica (AFnSi) crosslinker. The 3D β-TCP bioceramic compartment is used to provide essential mechanical support for cartilage regeneration in the long term and slow biodegradation. However, the apparent density and compressive strength of the 3D β-TCP bioceramics can be obtained for ~ 94.8% theoretical density and 11.38 ± 1.72 MPa, respectively. In addition, the in vivo results demonstrated that the hADSC + HG + 0.5AFnSi/3D β-TCP of the bilayer osteochondral graft showed a much better osteochondral defect repair outcome in a rabbit model. The other word, the subchondral bone scaffold of 3D β-TCP bioceramic could accelerate the bone formation and integration with the adjacent host cancellous tissue at 12 weeks after surgery. And then, a thicker cartilage layer with a smooth surface and uniformly aligned chondrocytes were observed by providing enough steady mechanical support of the 3D β-TCP bioceramic scaffold.
... 132 Factors such as pore size, swelling ratio and compression modulus should be characterized to elucidate the suitable parameters to induce chondrogenesis; in fact, pore size (75 ± 0.2 μm), swelling ratio (35%), and compression modulus (35 ± 1.5 kPa) of hyaluronic acid methacryloyl and gelatin methacryloyl hydrogels promotes human adiposederived stem cells differentiation into a chondrocyte lineage, stimulating SOX9, aggrecan, Col2α1-IIa and GAGs. 133 Similar cartilage specific genes have been up-regulated in polyglutamic acidsodium alginate hydrogels whit higher compression modulus (107 ± 8.6 kPa) and a toughness range of 481 ± 34 kJ/m 3 -2166 ± 63 kJ/m. 3,133 Regarding the parameters to stimulate hydrogels mechanically, it is relevant to consider type of mechanical stimuli and stimulation time; thus, human synovium-derived MSCs cultured into agarose increased the production of aggrecan and Col2α1 when a dynamic compression of 10 kPa/0.25 Hz was applied during 1 h/day till day 28. ...
... 133 Similar cartilage specific genes have been up-regulated in polyglutamic acidsodium alginate hydrogels whit higher compression modulus (107 ± 8.6 kPa) and a toughness range of 481 ± 34 kJ/m 3 -2166 ± 63 kJ/m. 3,133 Regarding the parameters to stimulate hydrogels mechanically, it is relevant to consider type of mechanical stimuli and stimulation time; thus, human synovium-derived MSCs cultured into agarose increased the production of aggrecan and Col2α1 when a dynamic compression of 10 kPa/0.25 Hz was applied during 1 h/day till day 28. 134 Similarly, intermittent unconfined dynamic compressive strains applied during 1 h/day for 3 weeks are suitable to induce pluripotent mesenchymal progenitor cells into chondrogenesis. ...
... 139 For this reason, measuring the crosslinking degree of hydrogel is a major challenge because features such as porosity, swelling behavior, and mechanical strength depend on the level of cross-linked chains. 85,97,133 Another challenge is to ensure that the hydrogel is biocompatible and does not elicit an adverse immune response or cytotoxicity; moreover, reduce inflammation is an important aspect of any scaffold-based therapy to ensure the hydrogel is accepted by the body and enhance chondrogenesis. 141 Hydrogels also should show good resilience when mechanical loads are applied, it means that scaffolds should exhibit a high deformation capacity under compression, indicating that it can withstand a large amount of strain without undergoing significant deformation. ...
Cartilage damage caused by trauma or osteoarthritis is a common joint disease that can increase the social and economic burden in society. Due to its avascular characteristics, the poor migration ability of chondrocytes, and a low number of progenitor cells, the self‐healing ability of cartilage defects has been significantly limited. Hydrogels have been developed into one of the most suitable biomaterials for the regeneration of cartilage because of its characteristics such as high‐water absorption, biodegradation, porosity, and biocompatibility similar to natural extracellular matrix. Therefore, the present review article presents a conceptual framework that summarizes the anatomical, molecular structure and biochemical properties of hyaline cartilage located in long bones: articular cartilage and growth plate. Moreover, the importance of preparation and application of hyaluronic acid – gelatin hydrogels for cartilage tissue engineering are included. Hydrogels possess benefits of stimulating the production of Agc1, Col2α1‐IIa, and SOX9, molecules important for the synthesis and composition of the extracellular matrix of cartilage. Accordingly, they are believed to be promising biomaterials of therapeutic alternatives to treat cartilage damage.
... Figure 2a). In addition, the methyl peaks on the methacrylate group appeared at 1.86 and 1.79 ppm, which were quite different from the methyl peaks on the disaccharide ring [20]. Compared with the FT-IR spectra of HA, the absorption peaks at 1715 ...
Scar-free wound healing is a challenging process due to the excessive deposition of extracellular matrix and collagen. To overcome this issue, hydrogels with superior biochemical and mechanical properties have been used in combination with medicinal compounds as wound dressings. In this study, a novel composite hydrogel consisting of double-crosslinked photocurable hyaluronic acid methacrylate (HAMA) and Laponite (Lap) loaded with bioactive bone morphogenetic protein 4 (BMP4) was developed and thoroughly characterized for its properties such as degradation, morphology, porosity, compression, skin adhesion, and load release. The effect of the HAMA/Lap/BMP4 hydrogel was evaluated through both in vitro and in vivo experiments. In the in vivo rabbit ear-scar model, the HAMA/Lap/BMP4 hydrogel dressing was found to reduce scar-related expressions of α-SAM and decrease the ratio of collagen Ι/III in wounded tissue. Additionally, histopathological examination indicated that the HAMA/Lap/BMP4 hydrogel-treated groups exhibited enhanced wound repair and increased levels of collagen maintenance compared to other standard groups, ultimately leading to scarless wound healing. Therefore, this sustained-release photocurable HAMA/Lap/BMP4 hydrogel offers a therapeutic approach for scar-free wound healing.
... Moreover, hybrid acrylated materials with biomimetic properties can be created to create biofunctional 3D constructs. For example, Nedunchezian et al. produced a novel photocurable hybrid hydrogel system comprising hyaluronic acid methacryloyl, GelMA, and acrylate-functionalized nano-silica crosslinkers for articular cartilage tissue engineering applications ( Fig. 2 D) [41] . They reported that their photocured hybrid hydrogel photocured hybrid hydrogel significantly increased chondrogenic marker gene expressions and enhanced type II collagen formation and the expression of sulfated glycosaminoglycan. ...
... (D) Schematic illustration of a novel photocurable hybrid hydrogel system comprising hyaluronic acid methacryloyl, GelMA, and acrylate-functionalized nano-silica crosslinkers used for articular cartilage tissue engineering applications. Reproduced from[41] . ...
Light-based bioprinting is a type of additive manufacturing technologies that uses light to control the formation of biomaterials, tissues, and organs. It has the potential to revolutionize the adopted approach in tissue engineering and regenerative medicine by allowing the creation of functional tissues and organs with high precision and control. The main chemical components of light-based bioprinting are activated polymers and photoinitiators. The general photocrosslinking mechanisms of biomaterials are described, along with the selection of polymers, functional group modifications, and photoinitiators. For activated polymers, acrylate polymers are ubiquitous but are made of cytotoxic reagents. A milder option that exists is based on norbornyl groups which are biocompatible and can be used in self-polymerization or with thiol reagents for more precision. Polyethylene-glycol and gelatin activated with both methods can have high cell viability rates. Photoinitiators can be divided into types I and II. The best performances for type I photoinitiators are produced under ultraviolet light. Most alternatives for visible-light-driven photoinitiators were of type II, and changing the co-initiator along the main reagent can fine-tune the process. This field is still underexplored and a vast room for improvements still exist, which can open the way for cheaper complexes to be developed. The progress, advantages, and shortcomings of light-based bioprinting are highlighted in this review, with special emphasis on developments and future trends of activated polymers and photoinitiators.
... It solidifies into gel within 10 s under visible light irradiation. Due to the portable forming method and good biocompatibility, materials based on HAMA have been applied to many biomedical fields, including in chondrocyte culture and cartilage regeneration, tumor model construction, drug-controlled release, microneedle preparation, wound dressing, biosensors, and postoperative anti-adhesion [45][46][47][48]. Galarraga et al. used a norbornene-modified hyaluronic acid (NorHA) macromer as a representative bioink and varied the printing parameters (e.g., capillary length, flow rate, light intensity) to identify printing conditions that were optimal for the ink [49], and then marrow mesenchymal stem cells (MSCs) were encapsulated in the bioink and the compound bioink was fabricated using photo irradiation for cartilage repair. ...
An osteochondral defect is a common and frequent disease in orthopedics and treatment effects are not good, which can be harmful to patients. Hydrogels have been applied in the repair of cartilage defects. Many studies have reported that hydrogels can effectively repair osteochondral defects through loaded cells or non-loaded cells. As a new type of hydrogel, photo-crosslinked hydrogel has been widely applied in more and more fields. Meanwhile, 3D bioprinting serves as an attractive platform to fabricate customized tissue-engineered substitutes from biomaterials and cells for the repair or replacement of injured tissues and organs. Although photo-crosslinkable hydrogel-based 3D bioprinting has some advantages for repairing bone cartilage defects, it also has some disadvantages. Our aim of this paper is to review the current status and prospect of photo-crosslinkable hydrogel-based 3D bioprinting for repairing osteochondral defects.
At present, articular cartilage repair and regeneration remains still one of the most concerned problems due to its poor self-healing capacity. Among the tissue engineering materials, hydrogel is considered as an ideal candidate due to its similarity to extracellular matrices. Despite the good biocompatibility of gelatin and hyaluronic acid hydrogels, they are still limited to serve as tissue engineering materials by fast degradation rate and poor mechanical performances. In order to solve these problems, novel polyvinyl alcohol/tannic acid/gelatin/hyaluronic acid (PTGH) hydrogels are prepared by a facile physical crosslinked method. The PTGH hydrogels exhibit the high moisture content (85%) and porosity (87%). Meanwhile, the porous microstructures and mechanical properties (compressive strength: 0.85 ∼ 2.59MPa; compressive modulus: 57.88 ∼ 124.27kPa) can be controlled by adjusting the mass ratio of PT/GH. In vitro degradation analysis shows that the PTGH hydrogels can be degraded gradually in PBS solution with the presence of lysozyme. For this gel system, based on the hydrogen bonds among molecules, it improved the mechanical properties of gelatin and hyaluronic acid hydrogels. With the degradation of PTGH hydrogels, the release of gelatin and hyaluronic acid can have a continuous effort for the cartilage tissue regeneration and repair. In addition, in vitro cell culture results show that the PTGH hydrogels have no negative effects on chondrocytes growth and proliferation. In all, the PTGH hydrogels exhibit the potential applications for articular cartilage tissue repair and regeneration.
Polysaccharides are naturally occurring polymers with exceptional biodegradable and biocompatible qualities that are used as hemostatic agents. In this study, photoinduced CC bond network and dynamic bond network binding was used to give polysaccharide-based hydrogels the requisite mechanical strength and tissue adhesion. The designed hydrogel was composed of modified carboxymethyl chitosan (CMCS-MA) and oxidized dextran (OD), and introduced hydrogen bond network through tannic acid (TA) doping. Halloysite nanotubes (HNTs) were also added, and the effects of various doping amount on the performance of the hydrogel were examined, in order to enhance the hemostatic property of hydrogel. Experiments on vitro degradation and swelling demonstrated the strong structural stability of hydrogels. The hydrogel has improved tissue adhesion strength, with a maximum adhesion strength of 157.9 kPa, and demonstrated improved compressive strength, with a maximum compressive strength of 80.9 kPa. Meanwhile, the hydrogel had a low hemolysis rate and had no inhibition on cell proliferation. The created hydrogel exhibited a significant aggregation effect on platelets and a reduced blood clotting index (BCI). Importantly, the hydrogel can quickly adhere to seal the wound and has good hemostatic effect in vivo. Our work successfully prepared a polysaccharide-based bio-adhesive hydrogel dressing with stable structure, appropriate mechanical strength, and good hemostatic properties.
