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The PCR result used 16S rRNA-specific primer for lactic acid bacteria. M, DNA molecular weight marker; 1, PCR product of Lpb. plantarum; 2, PCR product of L. sakei.

The PCR result used 16S rRNA-specific primer for lactic acid bacteria. M, DNA molecular weight marker; 1, PCR product of Lpb. plantarum; 2, PCR product of L. sakei.

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This study aimed to elucidate whether lactic acid bacteria (LAB) can be used as a bio-preservative and a natural antimicrobial agent to extend coconut oil shelf-life, even under stress conditions. Lactiplantibacillus plantarum and Latilactobacillus sakei were isolated from virgin coconut oil which was subjected to strong environmental stresses. L....

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Context 1
... 3.4×10 8 CFU/mL. The morphology of the MRS agar plate was presented as milky-white in color, circular, and convex (Astuti, 2016). Two species were identified as Lpb. plantarum and L. sakei, while the 16S rRNA specific for LAB was amplified using universal primers to confirm the species and the result showed a length of 1,500 bp as demonstrated in Fig. 1. The PCR result indicated that the band is clear enough without ...
Context 2
... 3.4×10 8 CFU/mL. The morphology of the MRS agar plate was presented as milky-white in color, circular, and convex (Astuti, 2016). Two species were identified as Lpb. plantarum and L. sakei, while the 16S rRNA specific for LAB was amplified using universal primers to confirm the species and the result showed a length of 1,500 bp as demonstrated in Fig. 1. The PCR result indicated that the band is clear enough without ...