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![The Chromosome Structural Abnormalities Found in Male and Female Leukocytes Induced by Silk Dye #CA5 and #30, (a) Incomplete Chromosome with Telomeric Association BetweenChromosome 9 and 12 [43,XY,-4,-5,tas(9;12)(p24;p13),-18], (b) Chromosome Loss and Rearrangement with 46,Y,-X,+mar, (c) Chromosome Loss and Rearrangement with 45,XY,t(2;12)(p25;p13),-7, (d) the Structural Chromosome Aberrations 46,XX,der(11)del(11)(q12) t(11;19)(q13;q13.4) and (e) 34,-X,-X,-1,-4,-9,-9,-10,10,-11,-12,-15,-19,+10 mar,5dmin.](publication/323426457/figure/fig4/AS:635599539167234@1528550147816/The-Chromosome-Structural-Abnormalities-Found-in-Male-and-Female-Leukocytes-Induced-by.png)
The Chromosome Structural Abnormalities Found in Male and Female Leukocytes Induced by Silk Dye #CA5 and #30, (a) Incomplete Chromosome with Telomeric Association BetweenChromosome 9 and 12 [43,XY,-4,-5,tas(9;12)(p24;p13),-18], (b) Chromosome Loss and Rearrangement with 46,Y,-X,+mar, (c) Chromosome Loss and Rearrangement with 45,XY,t(2;12)(p25;p13),-7, (d) the Structural Chromosome Aberrations 46,XX,der(11)del(11)(q12) t(11;19)(q13;q13.4) and (e) 34,-X,-X,-1,-4,-9,-9,-10,10,-11,-12,-15,-19,+10 mar,5dmin.
Source publication
Objectives: This study is aimed to assess the possible genotoxicity and mutagenicity of silk dyes on silk weavers.
Methods: Peripheral blood leukocytes were obtained from 24 silk weavers and 24 age- and sex-matched controls in
northeastern Thailand. After mitogen stimulation in culture, chromosome abnormalities were examined using Giemsa
banding an...
Contexts in source publication
Context 1
... the present study, not only genotoxicity but also the structural chromosome aberrations were observed in normal male leukocytes cultured with 50 and 250 μg/ mL of silk dye #CA5 Figure 4a, 4b, and 4c and in normal female leukocytes with 250 µg/mL of dye #30 Figure 4d and 4e. The induction of chromosome rearrangement indicates the mutagenicity of those silk dyes. Chromosome aberrations were found in several chromosome numbers and regions of the cultured normal leukocytes. Some of them had potential for molecular pathogenesis of several neoplasms. The presence of chromosome rearrangement indicates mutagenicity occurred because the genetic material alteration that can no longer be repaired by the cells. Chromosome aberrations found in blood cultured cells in this study involved several chromosome numbers and regions which many genes located on. Some of these mutations are involved in the molecular pathogenesis of various malignancies ( Keene et al., 1987;Cotterill, 2015;Ullah Shah et al., 2015;Wen et al., 2015 Asian Pacific Journal of Cancer Prevention, Vol 19 547 ...
Context 2
... the present study, not only genotoxicity but also the structural chromosome aberrations were observed in normal male leukocytes cultured with 50 and 250 μg/ mL of silk dye #CA5 Figure 4a, 4b, and 4c and in normal female leukocytes with 250 µg/mL of dye #30 Figure 4d and 4e. The induction of chromosome rearrangement indicates the mutagenicity of those silk dyes. Chromosome aberrations were found in several chromosome numbers and regions of the cultured normal leukocytes. Some of them had potential for molecular pathogenesis of several neoplasms. The presence of chromosome rearrangement indicates mutagenicity occurred because the genetic material alteration that can no longer be repaired by the cells. Chromosome aberrations found in blood cultured cells in this study involved several chromosome numbers and regions which many genes located on. Some of these mutations are involved in the molecular pathogenesis of various malignancies ( Keene et al., 1987;Cotterill, 2015;Ullah Shah et al., 2015;Wen et al., 2015 Asian Pacific Journal of Cancer Prevention, Vol 19 547 ...
Context 3
... characteristics of 24 silk weaving workers and 24 control subjects were shown in Table 1. The aberrant chromosome patterns found in this study were staying in prophase stage, incomplete chromosome forming, polyploidy Figure 2a-2c. The results of chromosome analyses of mitogen-stimulated cultured leukocytes of the silk weaving workers and control subjects were summarized in Table 2. The MI was not significantly different statistically of silk dyes to the culture and were significantly different among the concentration of dye added (p-value < 0.05; ANOVA: Two-Factor Without Replication). Chromosome aberrations such as incomplete chromosome forming and polyploidy were induced by silk dyes. A few structural changes of chromosomes were observed in various combination of the doses of dyes and male and female leukocytes. The silk dye #CA5 at 50 μg/mL induced 43,XY,-4,-5,tas(9;12) (p24;p13),-18 in male leukocytes (Figure 4a
...
Citations
... Reduced telomere length in workers was not associated with subject age and DNA damage markers but with exposure time to these harmful chemicals (Pavanello et al., 2010). Telomere shortening was linked with an increased risk of cancer, and workers exposed to harmful chemicals were at a higher risk of developing lung cancer (Kritpetcharat et al., 2018). Telomere shortening was also linked to ageing and shortened telomere length in coke oven workers was also associated with TP53 hypomethylation and it might increase p53 expression resulting in apoptotic cell death or growth inhibition (Pavanello et al., 2010). ...
Telomeres are often considered as the ‘ageing clock’ that determines the lifespan at the cellular level, forming the ends of a chromosome, which shorten each time the cell divides itself to the point where they become so short the cell is unable to divide itself further. Telomere length alteration is often linked with lifestyle factors such as age, obesity, exposure to pesticides and pollution, depression, unhealthy diet, lack of exercise, and stress. The current review discusses the mechanism of telomere shortening in relation to ageing and lifestyle factors in general and its association with chronic diseases like diabetes which may influence the health and lifespan of an individual by increasing telomere shortening. Accelerated or excessive telomere shortening is also associated with the early onset of age-related disorders globally and, hence, reduced lifespan of individuals. Upregulated Telomerase activity and reactivation of telomeres is observed in >70% of cancer patients by TERT point mutations, rearrangements, DNA amplifications, and transcript fusions, making it a useful marker in diagnosis and prognosis of various cancers. The study presents a systematic review of the unregulated Telomere activity with progression of various cancer and extrapolation of suitable pathways and prognostic information correlated with mRNA levels of TERT, which are critical among thymic epithelial tumors (TETs). In most cancers, unlimited proliferation is due to the reactivation of reverse transcriptase gene TERT. All these observations are comprehensively presented in the paper and might be useful for researchers working in the field of telomere dynamics and finding the correlation of age shortening with mRNA expression profiling.
... Adults and juveniles of tuco-tucos from Candiota had higher DNA damage than rodents of the same age group from Butiá (Fig. 4). Increase genetic alterations, such as chromosomal structural aberrations and aneuploidies, are associated with aging 52 . Increase as these, in the levels of cell damage associated with age, were found in whales exposed to PHAs, with the adults showing significantly more cytochrome P450 expression than juveniles 53 . ...
The Candiota coal mine in Rio Grande do Sul (RS) is one of the largest in Brazil. Coal is a fossil fuel that causes environmental impacts from its extraction to combustion due to the release of different agents, such as polycyclic aromatic hydrocarbons (PAH) and heavy metals. Ctenomys torquatus are herbivorous and subterranean rodents that dig tunnels with their paws and teeth and can be exposed to coal through contaminated food. Exposure to pollutants can cause DNA damage and affect different tissues, inducing alterations in the population structure and genetic diversity. Our study aimed to evaluate the effect of exposure to coal and its derivatives on the C. torquatus population and to examine the relationship of coal exposure with variations in absolute telomere length (aTL), global DNA methylation and genotoxicity. Our study showed an inverse correlation between telomere length and coal exposure in addition to an increase in DNA damage. The results indicate that coal and its byproducts can contribute to the alteration of the C. torquatus population structure, as evidenced by a reduction in the number of adults.
The genus Ctenomys has been widely used in karyotype evolution studies due to the variation in their diploid numbers (2n), which range from 2n = 10 to 2n = 70. Ctenomys minutus is characterized by intraspecific variation in diploid number (2n = 42, 46, 48, and 50), which makes it an interesting model to investigate the genomic instability mechanisms that have led to different cytotypes in this species. We aimed to contribute to the knowledge about telomeres’ role in chromosomal instability and global DNA methylation in the genome evolution of C. minutus . This study found that telomere length differs between cytotypes, but only for females (50a<46a,48a,42), although methylation was also higher, no significant difference was shown. It was also shown that young individuals, regardless of cytotype, had the longest telomere and the most methylated DNA, although only the last was statistically significant. Despite this, there is still much to be answered, although new cytotypes seem to have emerged within the distribution of parental cytotypes by the accumulation of different chromosomal rearrangements.
