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Faeces Vespertilionis is a commonly used fecal traditional Chinese medicine. Traditionally, it is identified relying only on morphological characters. This poses a serious challenge to the composition analysis accuracy of this complex biological mixture. Thus, for quality control purposes, an accurate and effective method should be provided for tax...
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... Notably, the parameters set in MEGAN included a minimum score of 50, a maximum expected value of 0.01, a top percent value of 10, a minimum support percent of 0.01, and a minimum support of 1 using the weighted LCA algorithm. Species-level taxonomic identity was assigned when the identity value between the query and reference sequences was ≥98 % (Zhang et al., 2022). ...
... However, selecting a suitable polymorphic locus and utilizing a robust DNA extraction approach are crucial for achieving high amplification success rates (Buś et al., 2014;Matsuoka et al., 2022). A subregion of COI was chosen for DNA barcoding of 21 individuals from 7 batches because it has been successfully applied in previous studies of medicinal herbs (Mansor et al., 2022;Verkuil et al., 2022;Zhang et al., 2022). The successful amplification and identification of all the individuals confirmed the suitability of the mini-barcode for our current study. ...
... MEGAN parameters were set as: minimum score = 50, maximum expected = 0.01, top percent = 10, minimum support percent = 0.01, minimum support = 1 and weighted LCA algorithm. Species-level taxonomy was assigned when the identity values between the query and reference sequences were above 98% [25]. The minimum identity to query would be set as 92% to obtain taxonomic information at a higher level for queries which could not be identified as exact species. ...
Mantidis Ootheca (Sangpiaoxiao, mantis egg case) is a typical multi-origin Chinese medicinal material. The Chinese Pharmacopoeia stipulates that the Mantidis Ootheca originates from three species of Mantis: Tenodera sinensis, Statilia maculate, and Hierodula patellifera. However, Mantidis Ootheca mainly relies on field collection, which leads to confusion of its actual origin in the market. As the clinical use of Mantidis Ootheca with unknown original mantis species will pose potential risks to drug safety, it is necessary to survey the commercially available Mantidis Ootheca origin species. However, as the egg case of Mantis, the morphological characters of Mantidis Ootheca are limited and usually cannot serve as accurate identification tool. DNA barcoding, which is widely used in taxonomic studies of animals, is severely affected by the impact of storage pests and DNA degradation. Thus, this study collected a total of 4580 Mantidis Ootheca and pooled separately Mantidis Ootheca samples according to 18 different sources as DNA samples to analyze the origin diversity of Mantidis Ootheca individuals contaminated by common store pests collected in in the market using DNA metabarcoding, and to provide a basis for quality control of Mantidis Ootheca. 37 Mantis ASVs and 9 Mantis MOTUs were identified through species delimitation, and the high-level intraspecific diversity was depicted as haplotype network plot. Besides Tenodera sinensis and Hierodula patellifera as genuine original mantis species defined in the Chinese Pharmacopoeia, Tenodera angustipennis was also the origin species of these Mantidis Ootheca samples.
... MEGAN parameters were set as: minimum score = 50, maximum expected = 0.01, top percent = 10, minimum support percent = 0.01, minimum support = 1 and weighted LCA algorithm. Species-level taxonomy was assigned when the identity values between the query and reference sequences were above 98% [24]. The minimum identity to query would be set as 92% to obtain taxonomic information at a higher level for queries which could not be identi ed as exact species. ...
Mantidis Ootheca (Sangpiaoxiao) is a typical multi-origin Chinese medicinal material. The Chinese Pharmacopoeia stipulates that the Mantidis Ootheca originates from Tenodera sinensis , Statilia maculate , and Hierodula patellifera . However, Mantidis Ootheca mainly relies on field collection, which leads to confusion of its actual origin in the market. As the clinical use of Mantidis Ootheca with unknown origin will pose potential risks to drug safety, it is necessary to survey the commercially available Mantidis Ootheca origin species. However, as the egg case of Mantis, the morphological characters of Mantidis Ootheca are limited and usually cannot serve as accurate identification tool. DNA barcoding, which is widely used in taxonomic studies of animals, is severely affected by the impact of storage pests and DNA degradation. Thus, this study aimed to analyze the origin diversity of 4,580 Mantidis Ootheca individuals contaminated by common store pests collected in the market using mini-barcode combined with DNA metabarcoding, and to provide a basis for quality control of Mantidis Ootheca. 37 Mantis ASVs and 9 Mantis MOTUs were identified through species delimitation, and the high level intraspecific diversity was depicted as haplotype network plot. Besides Tenodera sinensis and Hierodula patellifera as genuine sources defined in the Chinese Pharmacopoeia, Tenodera angustipennis was also the origin species of these Mantidis Ootheca samples.
