Symptoms of leaf spot on Sonchus asper caused by Alternaria sonchi. (A, B) Field symptoms: early symptoms of the disease (A) and severely infected symptoms of the disease (B). (C-E) Pathogenicity assay on 2-month-old Sonchus asper plant using A. sonchi suspension (1×10 5 spores/ml): initial symptoms on leaves post-24 hr-inoculation (C) and brown spots on plant petiole post-3 day-inoculation (D), and brown spots symptoms on leaves post-5 dayinoculation (E).

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... 2021). During the course of disease survey on weeds and minor cultivated crops in Korea in September 2019, a brown leaf spot disease of S. asper was observed in gardens and roadsides in Gongju and Nonsan, Chungnam Province, Korea. Leaf spots were round or irregular in shape, variable in size, grayish brown, or brown with a purple margin (Fig. 1A). The spots often enlarged and coalesced, resulting in blighting of the leaves (Fig. 1B), which is corresponded to the symptoms caused by genus Alternaria. In severe infection, incidence rate of the disease was more than ...

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... in Korea in September 2019, a brown leaf spot disease of S. asper was observed in gardens and roadsides in Gongju and Nonsan, Chungnam Province, Korea. Leaf spots were round or irregular in shape, variable in size, grayish brown, or brown with a purple margin (Fig. 1A). The spots often enlarged and coalesced, resulting in blighting of the leaves (Fig. 1B), which is corresponded to the symptoms caused by genus Alternaria. In severe infection, incidence rate of the disease was more than ...

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... sequences of ITS, RPB2, and TEF1 gene were identical to those of the representative isolate (CBS 119675) of A. sonchi from Sonchus plants. One and four nucleotides of our isolates were different from CBS 119675 based on GAPDH and Alt a1 sequences, respectively ( Supplementary Fig. 1A, B). Based on the combined phylogenetic tree, the three isolates had identical sequences for each of the five genes and formed a strongly supported clade (100% BS value) with the representative isolate (CBS 119675) of A. sonchi (Fig. 3). ...

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... tests. Initial symptoms on leaves of S. asper appeared 2-3 days after inoculation as small grayish brown spots, changing to pale yellowish-brown (Fig. 1C, D). Brown stripes were clearly observed on stems at 5 days after inoculation (Fig. 1E). All the symptoms resembled to those originally observed in the field. The same fungus was re-isolated from both leaves and stems and identified as the original inoculated fungus determined by morphological characteristics and phylogenetic analysis. ...

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... tests. Initial symptoms on leaves of S. asper appeared 2-3 days after inoculation as small grayish brown spots, changing to pale yellowish-brown (Fig. 1C, D). Brown stripes were clearly observed on stems at 5 days after inoculation (Fig. 1E). All the symptoms resembled to those originally observed in the field. The same fungus was re-isolated from both leaves and stems and identified as the original inoculated fungus determined by morphological characteristics and phylogenetic analysis. Control plants did not show any symptoms. The results in agreement with Koch's ...

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... 2021). During the course of disease survey on weeds and minor cultivated crops in Korea in September 2019, a brown leaf spot disease of S. asper was observed in gardens and roadsides in Gongju and Nonsan, Chungnam Province, Korea. Leaf spots were round or irregular in shape, variable in size, grayish brown, or brown with a purple margin (Fig. 1A). The spots often enlarged and coalesced, resulting in blighting of the leaves (Fig. 1B), which is corresponded to the symptoms caused by genus Alternaria. In severe infection, incidence rate of the disease was more than ...

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... in Korea in September 2019, a brown leaf spot disease of S. asper was observed in gardens and roadsides in Gongju and Nonsan, Chungnam Province, Korea. Leaf spots were round or irregular in shape, variable in size, grayish brown, or brown with a purple margin (Fig. 1A). The spots often enlarged and coalesced, resulting in blighting of the leaves (Fig. 1B), which is corresponded to the symptoms caused by genus Alternaria. In severe infection, incidence rate of the disease was more than ...

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... sequences of ITS, RPB2, and TEF1 gene were identical to those of the representative isolate (CBS 119675) of A. sonchi from Sonchus plants. One and four nucleotides of our isolates were different from CBS 119675 based on GAPDH and Alt a1 sequences, respectively ( Supplementary Fig. 1A, B). Based on the combined phylogenetic tree, the three isolates had identical sequences for each of the five genes and formed a strongly supported clade (100% BS value) with the representative isolate (CBS 119675) of A. sonchi (Fig. 3). ...

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... tests. Initial symptoms on leaves of S. asper appeared 2-3 days after inoculation as small grayish brown spots, changing to pale yellowish-brown (Fig. 1C, D). Brown stripes were clearly observed on stems at 5 days after inoculation (Fig. 1E). All the symptoms resembled to those originally observed in the field. The same fungus was re-isolated from both leaves and stems and identified as the original inoculated fungus determined by morphological characteristics and phylogenetic analysis. ...

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... tests. Initial symptoms on leaves of S. asper appeared 2-3 days after inoculation as small grayish brown spots, changing to pale yellowish-brown (Fig. 1C, D). Brown stripes were clearly observed on stems at 5 days after inoculation (Fig. 1E). All the symptoms resembled to those originally observed in the field. The same fungus was re-isolated from both leaves and stems and identified as the original inoculated fungus determined by morphological characteristics and phylogenetic analysis. Control plants did not show any symptoms. The results in agreement with Koch's ...