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Deniquelata barringtoniae gen. et sp. nov. (Montagnulaceae) forms numerous ascomata on distinct zonate leaf spots of Barringtonia asiatica (Lecythidaceae). We isolated this taxon and sequenced the 18S and 28S nrDNA. The result of phylogenetic analysis based on 18S and 28S nrDNA sequence data indicate that the genus belongs in the family Montagnulac...
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... entire to slightly undulated at the edge and remaining white to pinkish white (FIG. 3L−S). After 6 months of incubation, the colonies on malt extract agar and water agar media co ntained only superficial, branched, septate, smooth, mycelia with no asexual-stage. Distribution: On living leaves of Barringtonia asiatica Thailand. Pathogenicity test (FIG. 4): Leaves wounded by pinpricking inoculation technique, initially developed small, circular, ash-coloured spots which subsequently transformed into brown spots. After 10 days of incubation, the spots expanded to 2 mm diam. The spots further enlarged and became sunken causing soft decay of the leaf tissues, surrounded by white mycelium. ...
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Deniquelata barringtoniae gen. et sp. nov. (Montagnulaceae) forms numerous ascomata on distinct zonate leaf spots of Barringtonia asiatica (Lecythidaceae). We isolated this taxon and sequenced the 18S and 28S nrDNA. The result of phylogenetic analysis based on 18S and 28S nrDNA sequence data indicate that the genus belongs in the family Montagnulac...
Citations
... Maximum likelihood phylogenetic analysis was conducted from combined SSU + LSU + ITS + rpb2 + tef1-α sequence data of 156 strains, of which 14 were newly sequenced strains, while the other 142 strains were obtained from BLAST search (NCBI) and recent papers [50][51][52][53][54]. Our phylogenetic trees show similar topologies to the recent published papers [50][51][52][53][54]. ...
... Maximum likelihood phylogenetic analysis was conducted from combined SSU + LSU + ITS + rpb2 + tef1-α sequence data of 156 strains, of which 14 were newly sequenced strains, while the other 142 strains were obtained from BLAST search (NCBI) and recent papers [50][51][52][53][54]. Our phylogenetic trees show similar topologies to the recent published papers [50][51][52][53][54]. ...
... catimor in China, based on morphology and phylogeny. Notes: Deniquelata (D.) was introduced by Ariyawansa et al. [52], with D. barringtoniae as the type species, which was isolated as a pathogen, causing leaf spots of Barringtonia asiatica (Lecythidaceae). The sexual morph of Deniquelata is characterized by immersed ascomata, with an ostiolar, textura angularis cell peridium, bitunicate, clavate to broadly-clavate with short furcate pedicel asci and muriform ascospores, three transverse septa and 1-2 vertical septa, without a sheath [52,64]. ...
In Yunnan Province, the coffee-growing regions are mainly distributed in Pu’er and Xishuangbanna. During the surveys of microfungi associated with coffee in Yunnan Province, seven taxa were isolated from coffee samples. Based on molecular phylogenetic analyses of combined ITS, LSU, SSU, rpb2, and tef1-α sequence data and morphological characteristics, four new species viz. Deniquelata yunnanensis, Paraconiothyrium yunnanensis, Pseudocoleophoma puerensis, and Pse. Yunnanensis, and three new records viz. Austropleospora keteleeriae, Montagnula thailandica, and Xenocamarosporium acaciae in Pleosporales are introduced. In addition, Paracamarosporium fungicola was transferred back to Paraconiothyrium based on taxonomy and DNA sequences. Full descriptions, illustrations, and phylogenetic trees to show the placement of new and known taxa are provided. In addition, the morphological comparisons of new taxa with closely related taxa are given.
... nov. [11]. This is expected as many pathogens may induce similar symptoms and, therefore, there is the need to verify the causal agent in every instance. ...
This study was conducted to investigate a characteristic fungal disease, causing leaf anthracnose on B. edulis. A symptom progression from an angular-asymmetrical yellow discoloration to an ulcer-like necrosis caused death to the whole leaf. Colony pigmentations observed among the six isolates ranged from grey to white with slightly raised aerial mycelium to dense cottony mycelium. Most of the isolates showed salmon to bright orange spore-masses that were arranged in concentric rings, and with aging these orange spore-masses evenly distributed towards the culture peripheral. The conidial shapes of all of the isolates were straight and cylindrical with the average conidia length and width, ranged from 55.6-62.8 and 16.8-24µm. Together with differential tests using fungicide and a temperature regime revealed characteristics corresponding to Colletotrichum gloeosporioides.
... Notes -Deniquelata was established for a genus of Dothideomycetes with pseudothecia giving rise to bitunicate asci with pseudoparaphyses and brown, muriform ascospores (Ariyawansa et al. 2013). Deniquelata hypolithi is the first asexual morph linked to a species of Deniquelata. ...
Novel species of fungi described in this study include those from various countries as follows: Algeria,
Phaeoacremonium adelophialidum from Vitis vinifera. Antarctica, Comoclathris antarctica from soil. Australia,
Coniochaeta salicifolia as endophyte from healthy leaves of Geijera salicifolia, Eremothecium peggii in fruit of Citrus
australis, Microdochium ratticaudae from stem of Sporobolus natalensis, Neocelosporium corymbiae on stems of
Corymbia variegata, Phytophthora kelmanii from rhizosphere soil of Ptilotus pyramidatus, Pseudosydowia backhousiae
on living leaves of Backhousia citriodora, Pseudosydowia indooroopillyensis, Pseudosydowia louisecottisiae
and Pseudosydowia queenslandica on living leaves of Eucalyptus sp. Brazil, Absidia montepascoalis from soil.
Chile, Ilyonectria zarorii from soil under Maytenus boaria. Costa Rica, Colletotrichum filicis from an unidentified
fern. Croatia, Mollisia endogranulata on deteriorated hardwood. Czech Republic, Arcopilus navicularis from tea bag
with fruit tea, Neosetophoma buxi as endophyte from Buxus sempervirens, Xerochrysium bohemicum on surface
of biscuits with chocolate glaze and filled with jam. France, Entoloma cyaneobasale on basic to calcareous soil,
Fusarium aconidiale from Triticum aestivum, Fusarium juglandicola from buds of Juglans regia. Germany, Tetraploa
endophytica as endophyte from Microthlaspi perfoliatum roots. India, Castanediella ambae on leaves of Mangifera
indica, Lactifluus kanadii on soil under Castanopsis sp., Penicillium uttarakhandense from soil. Italy, Penicillium ferraniaense
from compost. Namibia, Bezerromyces gobabebensis on leaves of unidentified succulent, Cladosporium
stipagrostidicola on leaves of Stipagrostis sp., Cymostachys euphorbiae on leaves of Euphorbia sp., Deniquelata
hypolithi from hypolith under a rock, Hysterobrevium walvisbayicola on leaves of unidentified tree, Knufia hypolithi
and Knufia walvisbayicola from hypolith under a rock, Lapidomyces stipagrostidicola on leaves of Stipagrostis sp.,
Nothophaeotheca mirabibensis (incl. Nothophaeotheca gen. nov.) on persistent inflorescence remains of Blepharis
obmitrata, Paramyrothecium salvadorae on twigs of Salvadora persica, Preussia procaviicola on dung of Procavia
sp., Sordaria equicola on zebra dung, Volutella salvadorae on stems of Salvadora persica. Netherlands, Entoloma
ammophilum on sandy soil, Entoloma pseudocruentatum on nutrient poor (acid) soil, Entoloma pudens on
plant debris, amongst grasses. New Zealand, Amorocoelophoma neoregeliae from leaf spots of Neoregelia sp.,
Aquilomyces metrosideri and Septoriella callistemonis from stem discolouration and leaf spots of Metrosideros
sp., Cadophora neoregeliae from leaf spots of Neoregelia sp., Flexuomyces asteliae (incl. Flexuomyces gen. nov.)
and Mollisia asteliae from leaf spots of Astelia chathamica, Ophioceras freycinetiae from leaf spots of Freycinetia banksii, Phaeosphaeria caricis-sectae from leaf spots of Carex secta. Norway, Cuphophyllus flavipesoides on soil
in semi-natural grassland, Entoloma coracis on soil in calcareous Pinus and Tilia forests, Entoloma cyaneolilacinum
on soil semi-natural grasslands, Inocybe norvegica on gravelly soil. Pakistan, Butyriboletus parachinarensis on
soil in association with Quercus baloot. Poland, Hyalodendriella bialowiezensis on debris beneath fallen bark of
Norway spruce Picea abies. Russia, Bolbitius sibiricus on а moss covered rotting trunk of Populus tremula, Crepidotus
wasseri on debris of Populus tremula, Entoloma isborscanum on soil on calcareous grasslands, Entoloma
subcoracis on soil in subalpine grasslands, Hydropus lecythiocystis on rotted wood of Betula pendula, Meruliopsis
faginea on fallen dead branches of Fagus orientalis, Metschnikowia taurica from fruits of Ziziphus jujube, Suillus
praetermissus on soil, Teunia lichenophila as endophyte from Cladonia rangiferina. Slovakia, Hygrocybe fulgens
on mowed grassland, Pleuroflammula pannonica from corticated branches of Quercus sp. South Africa, Acrodontium
burrowsianum on leaves of unidentified Poaceae, Castanediella senegaliae on dead pods of Senegalia
ataxacantha, Cladophialophora behniae on leaves of Behnia sp., Colletotrichum cliviigenum on leaves of Clivia
sp., Diatrype dalbergiae on bark of Dalbergia armata, Falcocladium heteropyxidicola on leaves of Heteropyxis
canescens, Lapidomyces aloidendricola as epiphyte on brown stem of Aloidendron dichotomum, Lasionectria
sansevieriae and Phaeosphaeriopsis sansevieriae on leaves of Sansevieria hyacinthoides, Lylea dalbergiae on
Diatrype dalbergiae on bark of Dalbergia armata, Neochaetothyrina syzygii (incl. Neochaetothyrina gen. nov.) on
leaves of Syzygium chordatum, Nothophaeomoniella ekebergiae (incl. Nothophaeomoniella gen. nov.) on leaves of
Ekebergia pterophylla, Paracymostachys euphorbiae (incl. Paracymostachys gen. nov.) on leaf litter of Euphorbia
ingens, Paramycosphaerella pterocarpi on leaves of Pterocarpus angolensis, Paramycosphaerella syzygii on leaf
litter of Syzygium chordatum, Parateichospora phoenicicola (incl. Parateichospora gen. nov.) on leaves of Phoenix
reclinata, Seiridium syzygii on twigs of Syzygium chordatum, Setophoma syzygii on leaves of Syzygium sp., Starmerella
xylocopis from larval feed of an Afrotropical bee Xylocopa caffra, Teratosphaeria combreti on leaf litter of
Combretum kraussii, Teratosphaericola leucadendri on leaves of Leucadendron sp., Toxicocladosporium pterocarpi
on pods of Pterocarpus angolensis. Spain, Cortinarius bonachei with Quercus ilex in calcareus soils, Cortinarius
brunneovolvatus under Quercus ilex subsp. ballota in calcareous soil, Extremopsis radicicola (incl. Extremopsis
gen. nov.) from root-associated soil in a wet heathland, Russula quintanensis on acidic soils, Tubaria vulcanica on
volcanic lapilii material, Tuber zambonelliae in calcareus soil. Sweden, Elaphomyces borealis on soil under Pinus
sylvestris and Betula pubescens. Tanzania, Curvularia tanzanica on inflorescence of Cyperus aromaticus. Thailand,
Simplicillium niveum on Ophiocordyceps camponoti-leonardi on underside of unidentified dicotyledonous leaf. USA,
Calonectria californiensis on leaves of Umbellularia californica, Exophiala spartinae from surface sterilised roots of
Spartina alterniflora, Neophaeococcomyces oklahomaensis from outside wall of alcohol distillery. Vietnam, Fistulinella
aurantioflava on soil. Morphological and culture characteristics are supported by DNA barcodes.
... Notes -Deniquelata was established for a genus of Dothideomycetes with pseudothecia giving rise to bitunicate asci with pseudoparaphyses and brown, muriform ascospores (Ariyawansa et al. 2013). Deniquelata hypolithi is the first asexual morph linked to a species of Deniquelata. ...
Novel species of fungi described in this study include those from various countries as follows: Algeria , Phaeoacremonium adelophialidum from Vitis vinifera . Antarctica , Comoclathris antarctica from soil. Australia , Coniochaeta salicifolia as endophyte from healthy leaves of Geijera salicifolia , Eremothecium peggii in fruit of Citrus australis , Microdochium ratticaudae from stem of Sporobolus natalensis , Neocelosporium corymbiae on stems of Corymbia variegata , Phytophthora kelmanii from rhizosphere soil of Ptilotus pyramidatus , Pseudosydowia backhousiae on living leaves of Backhousia citriodora , Pseudosydowia indooroopillyensis , Pseudosydowia louisecottisiae and Pseudosydowia queenslandica on living leaves of Eucalyptus sp. Brazil , Absidia montepascoalis from soil. Chile , Ilyonectria zarorii from soil under Maytenus boaria . Costa Rica , Colletotrichum filicis from an unidentified fern. Croatia , Mollisia endogranulata on deteriorated hardwood. Czech Republic , Arcopilus navicularis from tea bag with fruit tea, Neosetophoma buxi as endophyte from Buxus sempervirens , Xerochrysium bohemicum on surface of biscuits with chocolate glaze and filled with jam. France , Entoloma cyaneobasale on basic to calcareous soil, Fusarium aconidiale from Triticum aestivum , Fusarium juglandicola from buds of Juglans regia . Germany , Tetraploa endophytica as endophyte from Microthlaspi perfoliatum roots. India , Castanediella ambae on leaves of Mangifera indica , Lactifluus kanadii on soil under Castanopsis sp., Penicillium uttarakhandense from soil. Italy , Penicillium ferraniaense from compost. Namibia , Bezerromyces gobabebensis on leaves of unidentified succulent, Cladosporium stipagrostidicola on leaves of Stipagrostis sp., Cymostachys euphorbiae on leaves of Euphorbia sp., Deniquelata hypolithi from hypolith under a rock, Hysterobrevium walvisbayicola on leaves of unidentified tree, Knufia hypolithi and Knufia walvisbayicola from hypolith under a rock, Lapidomyces stipagrostidicola on leaves of Stipagrostis sp., Nothophaeotheca mirabibensis (incl. Nothophaeotheca gen. nov.) on persistent inflorescence remains of Blepharis obmitrata , Paramyrothecium salvadorae on twigs of Salvadora persica , Preussia procaviicola on dung of Procavia sp., Sordaria equicola on zebra dung, Volutella salvadorae on stems of Salvadora persica . Netherlands , Entoloma ammophilum on sandy soil, Entoloma pseudocruentatum on nutrient poor (acid) soil, Entoloma pudens on plant debris, amongst grasses. New Zealand , Amorocoelophoma neoregeliae from leaf spots of Neoregelia sp., Aquilomyces metrosideri and Septoriella callistemonis from stem discolouration and leaf spots of Metrosideros sp., Cadophora neoregeliae from leaf spots of Neoregelia sp., Flexuomyces asteliae (incl. Flexuomyces gen. nov.) and Mollisia asteliae from leaf spots of Astelia chathamica , Ophioceras freycinetiae from leaf spots of Freycinetia banksii , Phaeosphaeria caricis-sectae from leaf spots of Carex secta . Norway , Cuphophyllus flavipesoides on soil in semi-natural grassland, Entoloma coracis on soil in calcareous Pinus and Tilia forests, Entoloma cyaneolilacinum on soil semi-natural grasslands, Inocybe norvegica on gravelly soil. Pakistan , Butyriboletus parachinarensis on soil in association with Quercus baloot . Poland , Hyalodendriella bialowiezensis on debris beneath fallen bark of Norway spruce Picea abies . Russia , Bolbitius sibiricus on а moss covered rotting trunk of Populus tremula , Crepidotus wasseri on debris of Populus tremula , Entoloma isborscanum on soil on calcareous grasslands, Entoloma subcoracis on soil in subalpine grasslands, Hydropus lecythiocystis on rotted wood of Betula pendula , Meruliopsis faginea on fallen dead branches of Fagus orientalis , Metschnikowia taurica from fruits of Ziziphus jujube , Suillus praetermissus on soil, Teunia lichenophila as endophyte from Cladonia rangiferina . Slovakia , Hygrocybe fulgens on mowed grassland, Pleuroflammula pannonica from corticated branches of Quercus sp. South Africa , Acrodontium burrowsianum on leaves of unidentified Poaceae , Castanediella senegaliae on dead pods of Senegalia ataxacantha , Cladophialophora behniae on leaves of Behnia sp., Colletotrichum cliviigenum on leaves of Clivia sp., Diatrype dalbergiae on bark of Dalbergia armata , Falcocladium heteropyxidicola on leaves of Heteropyxis canescens , Lapidomyces aloidendricola as epiphyte on brown stem of Aloidendron dichotomum , Lasionectria sansevieriae and Phaeosphaeriopsis sansevieriae on leaves of Sansevieria hyacinthoides , Lylea dalbergiae on Diatrype dalbergiae on bark of Dalbergia armata , Neochaetothyrina syzygii (incl. Neochaetothyrina gen. nov.) on leaves of Syzygium chordatum , Nothophaeomoniella ekebergiae (incl. Nothophaeomoniella gen. nov.) on leaves of Ekebergia pterophylla , Paracymostachys euphorbiae (incl. Paracymostachys gen. nov.) on leaf litter of Euphorbia ingens , Paramycosphaerella pterocarpi on leaves of Pterocarpus angolensis , Paramycosphaerella syzygii on leaf litter of Syzygium chordatum , Parateichospora phoenicicola (incl. Parateichospora gen. nov.) on leaves of Phoenix reclinata , Seiridium syzygii on twigs of Syzygium chordatum , Setophoma syzygii on leaves of Syzygium sp., Starmerella xylocopis from larval feed of an Afrotropical bee Xylocopa caffra , Teratosphaeria combreti on leaf litter of Combretum kraussii , Teratosphaericola leucadendri on leaves of Leucadendron sp., Toxicocladosporium pterocarpi on pods of Pterocarpus angolensis . Spain , Cortinarius bonachei with Quercus ilex in calcareus soils, Cortinarius brunneovolvatus under Quercus ilex subsp. ballota in calcareous soil, Extremopsis radicicola (incl. Extremopsis gen. nov.) from root-associated soil in a wet heathland, Russula quintanensis on acidic soils, Tubaria vulcanica on volcanic lapilii material, Tuber zambonelliae in calcareus soil. Sweden , Elaphomyces borealis on soil under Pinus sylvestris and Betula pubescens . Tanzania , Curvularia tanzanica on inflorescence of Cyperus aromaticus . Thailand , Simplicillium niveum on Ophiocordyceps camponoti-leonardi on underside of unidentified dicotyledonous leaf. USA , Calonectria californiensis on leaves of Umbellularia californica , Exophiala spartinae from surface sterilised roots of Spartina alterniflora , Neophaeococcomyces oklahomaensis from outside wall of alcohol distillery. Vietnam , Fistulinella aurantioflava on soil. Morphological and culture characteristics are supported by DNA barcodes.
... We keep Curreya in Didymosphaeriaceae, but recollection of the type specimens and molecular data from type strains are essential to resolve the placement of this genus. Notes -Deniquelata was introduced by Ariyawansa et al. (2013c) to accommodate Deniquelata barringtoniae as the type species. Deniquelata species are characterized by immersed, dark brown to black ascomata, with bitunicate asci and brown, muriform ascospores (Ariyawansa et al. 2014d). ...
... Deniquelata species are characterized by immersed, dark brown to black ascomata, with bitunicate asci and brown, muriform ascospores (Ariyawansa et al. 2014d). Deniquelata is considered as a pathogenic genus with fruiting bodies scattered in the necrotic host tissues (Ariyawansa et al. 2013c(Ariyawansa et al. , 2014d. Only three Deniquelata species (D. barringtoniae, D. quercina and D. vittalii) have been recorded in this genus (Species Fungorum 2020). ...
... Didymosphaeriaceae include some genera which are of economic importance, since they play a negative role by causing plant diseases, such as Austropleospora, Barria and Deniquelata (Ariyawansa et al. 2013c, 2014d, Wijayawardene et al. 2017a). In particular, Ariyawansa et al. (2013c) has proved the pathogenicity of Deniquelata by pinpricking inoculation technique on Barringtonia asiatica leaves. ...
... Didymosphaeriaceae was erected by Munk (1953) within the order Pleosporales based on the genus Didymosphaeria Fuckel (Aptroot 1995). The family contains terrestrial, aquatic, saprotrophic, parasitic, and hemibiotrophic taxa, currently arranged in more than 25 genera (Ariyawansa et al. 2014a(Ariyawansa et al. , 2014b, of which six genera viz., Alloconiothyrium, Bimuria, Deniquelata, Didymocrea, Verrucoconiothyrium, and Xenocamarosporium are monotypic (Ariyawansa et al. 2013, Liu et al. 2015, Crous et al. 2015. Ariyawansa and colleagues (Ariyawansa et al. 2014a(Ariyawansa et al. , 2014b have provided a comprehensive refinement of the taxonomy of the family, suggesting that Montagnulaceae and Didymosphaeriaceae are synonymous. ...
Didymosphaeriaceae is a cosmopolitan family of Ascomycetes including pathogens, saprobes, and endophytes living in a variety of substrates. While a monophyletic origin of the family is now widely accepted, the monophyly of some species, their worldwide distribution, and the timing of their origin is still disputed or unexplored. We first investigated Didymosphaeriaceae species diversity in six provinces of Iran with special focus on East Azarbaijan province: based on morphological features and analyses of concatenated internal transcribed spacer regions of the rDNA operon (ITS), large subunit rDNA (LSU) and beta-tubulin, only eight samples out of 1150 contained Didymosphaeroid fungi belonging to either Paraconiothyrium fuckelii or Kalmusia variispora; our sampling indicates 4 plants as new natural hosts of these fungi. We then performed an updated phylogenetic analysis of the Didymosphaeriaceae. Results indicate that the genera Paraconiothyrium and Kulmasia are polyphyletic as the likely result of previous species mis-assignments. We finally provided the first divergence estimates of the Didymosphaeriaceae using ITS. Bayesian relaxed clock analyses support an ancient (early Paleogene) origin of the family and a recent (late Neogene) diversification of extant genus compatible with a high loss of diversity in the past and/or of a still fragmented sampling of Didymosphaeriaceae diversity.
... Ascospores are oblong to narrowly oblong, reddish brown to dark yellowish brown, muriform with three transverse septa and 1-2 vertical septa, verruculose and lacking a sheath ). This genus currently includes two species, D. barringtoniae and D. vittalii Devadatha, V.V Sarma, E.B.G Jones ( Ariyawansa et al. 2013, Devadatha et al. 2018. Deniquelata barringtoniae is a pathogenic species causing necrosis on living leaves of Barringtonia asiatica and D. vittalii has been recorded on decaying woody stem of the Suaeda monoica (Devadatha et al. 2018). ...
In this study, a new endophyte species within the genus Deniquelata (Didymosphaeriaceae) was isolated from the branches of Persian oak trees (Quercus brantii) in Zagros forests, Iran. The maximum parsimony and Bayesian analyses of 18S, 28S and ITS, rDNA sequence data revealed that the isolates were distinct from other species of the Deniquelata. Based on molecular phylogenetic and morphological characteristics, this fungus was demonstrated to be a new species, Deniquelata quercina. Detailed descriptions and illustrations were provided for D. quercina and it was compared with other known species in the genus.
... The monotypic genus Deniquelata was erected by Ariyawansa et al. (2013) to accommodate D. barringtoniae Ariyawansa & K.D. Hyde, which was collected from a leaf of Barringtonia asiatica in Chiang Rai, Thailand. We found another collection of Deniquelata barringtoniae on dead leaves of Pandanus sp. from Prachuap Khiri Khan in Thailand. ...
... Notes: In the molecular analysis our isolate clustered with Deniquelata barringtoniae (CBS 109027) with high bootstrap support (100% in ML, 1 in BYPP, Fig. 13). The morphology of our isolate was similar to that of D. barringtoniae described by Ariyawansa et al. (2013), although we note that the ascospores have a mucilaginous sheath. This is the first report of Deniquelata from Pandanaceae. ...
This paper provides illustrated descriptions of micro-fungi newly found on Pandanaceae in China and Thailand. The fungi are accommodated in 31 families. New taxa described include a new family, seven new genera, 65 new species, 16 previously known species. A new family: Malaysiascaceae (Glomerellales). New genera are Acremoniisimulans (Plectosphaerellaceae), Pandanaceomyces, Pseudoachroiostachy (Nectriaceae), Pseudohyaloseta (Niessliaceae), Pseudoornatispora (Stachybotriaceae) and Yunnanomyces (Sympoventuriaceae). New species are Acremoniisimulans thailandensis, Beltrania krabiensis, Beltraniella pandanicola, B. thailandicus, Canalisporium krabiense, C. thailandensis, Clonostachys krabiensis, Curvularia chonburiensis, C. pandanicola, C. thailandicum, C. xishuangbannaensis, Cylindrocladiella xishuangbannaensis, Dictyochaeta pandanicola, Dictyocheirospora nabanheensis, D. pandanicola, D. xishuangbannaensis, Dictyosporium appendiculatum, Di. guttulatum, Di. hongkongensis, Di. krabiense, Di. pandanicola, Distoseptispora thailandica, D. xishuangbannaensis, Helicoma freycinetiae, Hermatomyces biconisporus, Lasiodiplodia chonburiensis, L. pandanicola, Lasionectria krabiense, Menisporopsis pandanicola, Montagnula krabiensis, Musicillium pandanicola, Neofusicoccum pandanicola, Neohelicomyces pandanicola, Neooccultibambusa thailandensis, Neopestalotiopsis chiangmaiensis, N. pandanicola, N. phangngaensis, Pandanaceomyces krabiensis, Paracylindrocarpon nabanheensis, P. pandanicola, P. xishuangbannaensis, Parasarcopodium hongkongensis, Pestalotiopsis krabiensis, P. pandanicola, Polyplosphaeria nabanheensis, P. pandanicola, P. xishuangbannaensis, Pseudoachroiostachys krabiense, Pseudoberkleasmium pandanicola, Pseudochaetosphaeronema pandanicola, Pseudohyaloseta pandanicola, Pseudoornatispora krabiense, Pseudopithomyces pandanicola, Rostriconidium pandanicola, Sirastachys phangngaensis, Stictis pandanicola, Terriera pandanicola, Thozetella pandanicola, Tubeufia freycinetiae, T. parvispora, T. pandanicola, Vermiculariopsiella hongkongensis, Volutella krabiense, V. thailandensis and Yunnanomyces pandanicola. Previous studies of micro-fungi on Pandanaceae have not included phylogenetic support. Inspiration for this study came from the book Fungi Associated with Pandanaceae by Whitton, McKenzie and Hyde in 2012. Both studies reveal that the micro-fungi on Pandanaceae is particularly rich in hyphomycetes. All data presented herein are based on morphological examination of specimens, coupled with phylogenetic sequence data to better integrate taxa into appropriate taxonomic ranks and infer their evolutionary relationships.
... However, recent studies have resolved our understanding. For example, Ariyawansa et al. (2013c) introduced the new generic name Deniquelata, with muriform ascospores in the family Montagnulaceae based on a combined dataset of 18S and 28S nrDNA sequences. Murispora was introduced based on Pleospora rubicunda and referred to Pleosporaceae. ...
... Indeed, the International Commission on the Taxonomy of Fungi (ICTF; Seifert and Rossman 2010) now urges those describing new species to endeavour to obtain cultures and molecular data. For example, based on a combined dataset of 18S and 28S nrDNA sequences, Ariyawansa et al. (2013c) introduced the new generic name Deniquelata in the family Montagnulaceae; and Liu et al. (2011b) reported two new genera, Fissuroma and Neoastrosphaeriella, in the family Aigialaceaein both cases their decisions were based on distinguishing morphologies and molecular phylogenetic analyses. Most molecular studies carried out before 2000 used fungal analyses to develop techniques and made little progress towards solving taxonomic problems (Hibbett et al. 2007;Nilsson et al. 2008;Schoch et al. 2009). ...
A review of phylogenetic studies carried out together
with morphological ones shows that a major problem
with most early studies is that they concentrated on techniques
and used material or strains of fungi that in most cases were
not carefully reference, and in a worrying number of cases
wrongly named. Most classical species, particularly of
microfungi, are not represented by adequate type material, or
other authoritatively identified cultures or specimens, that can
serve as DNA sources for phylogenetic study, or for
developing robust identification systems. Natural classifications
of fungi therefore suffer from the lack of reference strains
in resultant phylogenetic trees. In some cases, epitypification
and neotypification can solve this problem and these tools are
increasingly used to resolve taxonomic confusion and stabilize
the understanding of species, genera, families, or orders of
fungi. This manuscript discusses epitypification and
neotypification, describes how to epitypify or neotypify species
and examines the importance of this process. A set of guidelines for epitypification is presented. Examples where
taxa have been epitypified are presented and the benefits and
problems of epitypification are discussed. As examples of
epitypification, or to provide reference specimens, a new
epitype is designated for Paraphaeosphaeria michotii and
reference specimens are provided for Astrosphaeriella
stellata, A. bakeriana, Phaeosphaeria elongata, Ophiobolus
cirsii, and O. erythrosporus. In this way we demonstrate how
to epitypify taxa and its importance, and also illustrate the
value of proposing reference specimens if epitypification is
not advisable. Although we provided guidelines for
epitypification, the decision to epitypify or not lies with the
author, who should have experience of the fungus concerned.
This responsibility is to be taken seriously, as once a later
typification is made, it may not be possible to undo that,
particularly in the case of epitypes, without using the lengthy
and tedious formal conservation and rejection processes.
... The specimens were collected from five locations in three provinces in southern China (Hainan, Yunnan, Guizhou), and taken to the laboratory in plastic bags. The samples were processed and examined following the methods of Ariyawansa et al. 34 . Fresh and herbarium materials were examined using an Olympus SZX10 dissecting microscope to locate sporulating structures. ...
During our continuous survey (2012–2016) of saprobic hyphomycetes from dead branches in the forest ecosystems of southern China, we collected several acrodictys-like species. Acrodictys-like species are characterized by darkly pigmented and muriform conidia produced from holoblastic conidiogenous cells on macronematous, mononematous, cylindrical and unbranched or infrequently branched conidiophores. Phylogenetic analyses of ncLSU, ncSSU, ITS and tub2 sequence data lead us to propose two novel families in Sordariomycetes, Acrodictyaceae and Junewangiaceae. In addition, a new species, Acrodictys hainanensis, two new combinations, Junewangia queenslandica and Distoseptispora martinii, three new Chinese records, Acrodictys liputii, A. peruamazonensis and Junewangia sphaerospora are introduced. Two names, Acrodictys globulosa and A. malabarica, are resurrected.
