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Summer squash seed moisture content after priming in different osmotic solutions and after drying.
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Salinity impairs seed germination and reduces crop yield. Salt and osmotic stresses are responsible for both inhibition or delayed seed germination and seedling establishment. Seed priming is one of the physiological methods that improve seed performance under salinity. This research was carried out in order to assess the effect of seed priming on...
Contexts in source publication
Context 1
... squash seeds (cultivar S-12) were primed in solution of polyethylene glycol (PEG 6000) at -2 MPa, KNO 3 at -1.27 MPa and NaCl at -1.5 MPa in a dark room ( Table 1). 100 seeds were placed in 300 ml of each solution in a beaker of 500 ml sealed with Para film with a hole in the top to allow for air supply by little pipes (Mauromicale and Cavallaro 1996). ...
Context 2
... priming, summer squash seeds were immersed in priming solution at 20°C for 24 h in the dark. Following treatments, the seeds were washed three times for 5 min each in distilled water, and then dried with blotting paper and subsequently in a flow of dry air at 30°C, until the original moisture content was approxi- mately reached ( Table 1). ...
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Citations
... Priming with KNO3 (500µgmL -1 ) lead to 100% germination. A similar increase in germination percentage in cucurbit seeds primed with KNO3 solution has been reported in ash gourd (Rahman et al., 2014), Cucurbita pepo (Gharahlar et al., 2009) and watermelon (Demir and Mavi, 2004). ...
... Similarly, in seeds stored for 45 and 60 DAH and subjected to priming treatments, significant increase in seedling length was recorded. Likewise, increased in shoot length when primed with KNO3 solution was recorded in summer squash (Gharahlar et al., 2009), watermelon (Barbosa et al., 2016) and Cucumis sativus (Okon and Okon, 2017) seeds. Seed priming with GA3 also have beneficial effect on improving seedling growth in a range of plant species (Kandil et al., 2014). ...
... Germination rate in a seed lot measures the length of time it takes a seed to germinate. Seeds of round gourd exhibit maximum value of coefficient of rate of germination when subjected to priming with 500 µgmL -1 KNO3 for 12 and 24 (Demir and Mavi, 2014), and summer squash (Gharahlar et al., 2009). However, it has been found that priming has significant role in increasing the speed of germination e.g., in bitter gourd (Mehta et al., 2014), and cucumber (Mehta et al., 2013) seeds. ...
Owing to its hard seed coat, the seeds of Praecitrullus fistulosus exhibit dormancy which impairs the germinability and subsequent seed establishment. To overcome this dormancy, the seeds are required to be stored for a minimum of 45 days after harvest (DAH). In north Indian plains, round gourd seeds are harvested in mid-June and are required to be sown immediately in June-July. Therefore, priming treatments were employed to overcome seed dormancy in a short interval. To assess the effect of various priming treatments in overcoming dormancy, the seeds were stored at room temperature for various durations and at the end of the storage period, the seeds were primed by soaking in solutions of GA3 (150 µgmL-1, 500 µgmL-1,1000 µgmL-1 gibberellic acid), cytokinin (150 µgmL-1, 500 µgmL-1,1000 µgmL-1kinetin), ethylene (150, 500, 1000 ethrel), KNO3 (150 and 500 µgmL-1), HNO3 (150 and 500 µlL-1) and water, at 250C for 12 hours and 24 hours, respectively. Among all the priming treatments, 500 µgmL-1 KNO3 for 24 h enhanced percentage germination to more than 60 per cent in freshly harvested seeds to more than 80 per cent in seeds stored for 15 DAH. It also enhanced the length of seedling, vigour index (SVI-I and SVI-II), and coefficient of rate of germination (CRG) with a reduction in the mean days taken for germination (MDG).
... Priming dapat juga dilakukan dengan menggunakan asam giberelat (GA). Giberelin merupakan hormon tanaman yang berproduksi di akar tanaman dan berperan penting dalam pertumbuhan dan perkembangan tanaman mentimun (Shahi et al. 2009). Perendaman menggunakan GA3 dapat merangsang munculnya tunas, meningkatkan pertumbuhan dan mengaktifkan enzim (Khan et al. 2009;Haghpanah et al. 2009). ...
Selain faktor genetik dan lingkungan, faktor fisiologi benih juga sangat berpengaruh terhadap potensi hasil tanaman terutama pada kemampuannya menghadapi cekaman lingkungan. Penelitian ini bertujuan untuk mengetahui pengaruh priming benih dengan variasi konsentrasi NaCl pada viabilitas benih yang ditanam pada media salin. Penelitian ini dilaksanakan di Kabupaten Maros pada bulan April hingga Juli 2021. Penelitian dilakukan dalam dua tahap percobaan. Tahap pertama adalah tahap priming benih untuk pengujian perkecambahan benih. Menggunakan Rancangan Acak Lengkap (RAL), hasil penelitian tahap ini menunjukkan bahwa walaupun perlakuan kontrol memberikan hasil terbaik pada parameter persentase daya berkecambah dan indeks vigor benih, namun tidak berbeda nyata dengan benih yang telah melalui proses priming. Tahap kedua adalah uji toleransi salinitas menggunakan rancangan factorial dua faktor. Hasil penelitian menunjukkan priming benih dengan NaCl 0.5 % maupun 1.0% secara nyata mampu meningkatkan toleransi tanaman kacang hijau terhadap cekaman salinitas. Walaupun pada proses perkecambahan adanya priming benih dengan larutan NaCl memperlihatkan hasil yang lebih rendah dibanding kontrol, namun proses priming tampaknya memberi induksi pada benih untuk meningkatkan toleransi tanaman pada cekaman salinitas jika dilihat dari respon pertumbuhan dan produksi tanaman.
... The effectiveness of seed priming with inorganic salt, such as CaCl2, KCl, KNO3, and MgSO4, for overcoming salinity stress has been noted in a variety of crop species (Table 1) [4,[114][115][116][117]. Shahi-Gharahlar et al. [117] reported that priming with PEG was less effective for decreasing salinity damage than with NaCl in summer squash (Cucurbita pepo L.) [117]. ...
... The effectiveness of seed priming with inorganic salt, such as CaCl2, KCl, KNO3, and MgSO4, for overcoming salinity stress has been noted in a variety of crop species (Table 1) [4,[114][115][116][117]. Shahi-Gharahlar et al. [117] reported that priming with PEG was less effective for decreasing salinity damage than with NaCl in summer squash (Cucurbita pepo L.) [117]. Nevertheless, extensive studies revealed that PEG priming is a successful approach to improve germination performance in various plant species including amaranth (Amaranthus spp.) [118], pepper (Capsicum annuum L.) [119], tomato (Solanum lycopersicum L.) [120], sugarcane (Saccharum officinarum L.) [121], soybean [122], wheat [123], and sweet corn [83] under salinity stress. ...
... The effectiveness of seed priming with inorganic salt, such as CaCl2, KCl, KNO3, and MgSO4, for overcoming salinity stress has been noted in a variety of crop species (Table 1) [4,[114][115][116][117]. Shahi-Gharahlar et al. [117] reported that priming with PEG was less effective for decreasing salinity damage than with NaCl in summer squash (Cucurbita pepo L.) [117]. Nevertheless, extensive studies revealed that PEG priming is a successful approach to improve germination performance in various plant species including amaranth (Amaranthus spp.) [118], pepper (Capsicum annuum L.) [119], tomato (Solanum lycopersicum L.) [120], sugarcane (Saccharum officinarum L.) [121], soybean [122], wheat [123], and sweet corn [83] under salinity stress. ...
Abiotic stresses such as drought, extreme temperature, and salinity can negatively impact seed germination and plant growth and have become major limitations to crop production. Most crops are vulnerable to abiotic stress factors during their early growth phase, especially during seed germination and seedling emergence. Rapid crop seed germination and seedling establishment is known to provide competitive advantages over weeds and improve yields. Seed osmopriming is defined as a pre-sowing treatment in which seeds are soaked in osmotic solutions to undergo the first stage of germination, but radicle protrusion has not occurred. The process of osmopriming involves prior exposure of seeds in low-water-potential solutions. Osmopriming can generate a series of pre-germination metabolic activities, increase the antioxidant system activities, and prepare the seed for radicle protrusion. Polyethylene glycol (PEG) is a popular osmopriming agent that can alleviate the negative impacts of abiotic stresses. This review summarizes research findings on crop responses to seed priming with PEG under abiotic stresses. The challenges, limitations, and opportunities of using PEG for crop seed priming are discussed with the goal of providing insights into future research towards effective application of seed priming in crop production.
... These results are in line with the findings on many crops like melon (Sivritepe et al. 2003), canola (Farhoudi et al. 2007), pepper (Khan et al. 2009), tall fescue (Tilaki et al. 2010), safflower (Farhoudi and Motamedi 2010), sunflower (Bajehbaj 2010) and pot marigold (Sedghi et al. 2010). Also, Iqbal et al. (2006) on wheat and Shahi et al. (2009) on squash suggested that seed priming by NaCl is an effective method in alleviating the adverse effects of salt stress. Similarly, Katembe et al. (1998) have investigated the effect of NaCl as a priming agent on germination rate and seedling germination of two Atriplex species reduced under salt stress. ...
Background: Optimum benefits of seed priming on four cultivars of coriander (Coriandrum sativum) having different origin (Tunisia, Algeria, Egypt, and Syria) were studied under salinity stress.
Materials and Methods: Seeds of coriander were primed with NaCl at five levels (0, 2, 4, 6 and 8 g L-1) for three different times (12, 24 and 36 h) at 25°C. Then primed (P) and non-primed (NP) seeds were irrigated with five different saline solutions consisting of 0 (control), 2, 4, 6 and 8 g L-1 NaCl.
Results: The results showed that the best germination percentage was obtained by applying NaCl at 4 g L-1 for 12 h. Also, NaCl priming increased germination percentage compared with non-primed seeds. Overall increased NaCl level led to the reductions in the traits under study but these reductions were higher for non-primed compared to primed seeds. An Egyptian cultivar seemed to be the most sensitive.
Conclusions: The present study revealed that under salinity seed priming with NaCl could be used as a method to improve seed performance in coriander. However, further studies are needed to investigate the effects of NaCl priming on later growth and development of this crop.
... Plant species, osmotic potential of solution, duration of priming, temperature, seed vigor, the rate of seed refrying and the storage conditions are effective in the success of seed priming (Parera and Cantliffe, 1994). The beneficial effects of priming have been reported for many plants such as isabgol (Ghassemi-Golezani et al., 2011a), marigold (Ganji-Arjenaki et al., 2011), wheat (Amin et al., 2012), rapeseed (Mohammadi and Amiri, 2010; Jabbarpour et al., 2012), chickpea (GhassemiGolezani et al., 2008b), safflower (Aymen et al., 2012) and summer squash (Shahi-Gharahlar et al., 2009). This research was carried out to evaluate the effects of hydro and salt priming on field performance of borage under full and limited irrigation conditions. ...
Among the different environmental constraints, drought is the most limiting factor for plant productivity. Seed priming may improve plant performance under adverse environmental conditions. Thus, an experiment was conducted in 2012 to investigate field performance of borage in response to seed priming and water limitation. Irrigation treatments (I 1 , I 2 , I 3 and I 4 : irrigation after 60, 90, 120 and 150 mm evaporation from class A pan, respectively) and seed priming methods [P 1 (unprimed), P 2 (hydro-priming), P 3 (salt-priming with 1% KNO 3) and P 4 (salt-priming with 1% KH 2 PO 4) for 8 h at 15±1ºC] were allocated to main and sub plots, respectively. Percentage ground cover (PGC) under I 1 and I 2 was considerably higher than that under I 3 and I 4 . PGC slightly improved as a result of seed priming, particularly hydro-priming. Decreasing water supply led to 5-34.7 % reduction in biological yield and 34.3-57.3 % reduction in grain yield. Harvest index under severe water deficit (I 4) was 34.2 % less than that under well irrigation (I 1). The highest and the lowest harvest indices were recorded for P 2 and P 1 plants. Grain yield of plants from primed seeds was 22.5-60.1% higher than that of control (P 1). Improvement in grain yield of plants from primed seeds was attributed to higher ground green cover of these plants, compared with those from unprimed seeds.
... Under saline conditions, seed priming with KNO 3 (-1.27 MPa at EC=7 ds/m) was better than NaCl and PEG priming treatments because seedling fresh weight, root and shoot length increased in summer squash (Cucurbita pepo) (Shahi-Gharahlar et al. 2009). According to the results of this study, KNO 3 and NaCl at 100 mmol/L had the best effect on seed germination of F. rubra; however, when compared with the control, 50 mmol/L of any salt source, and 100 mmol/L of CaCl 2 showed no significant differences. ...
... Nascimento (2003) reported that muskmelon (Cucumis melo L.) seed priming with KNO 3 increased SFW under low temperature (at 17°C) stress in compared to non-primed seeds. Shahi-Gharahlar et al. (2009) suggested that, under saline conditions, seed priming with KNO 3 was better than other priming treatments (NaCl and PEG), because SFW, root and shoot length increased in summer squash (Cucurbita pepo) seedlings. Okcu et al. (2005) observed a remarkable decrease in SFW of pea (Pisum sativum L.) caused by a decrease in water potential when NaCl and PEG were used. ...
Salinity is one of the major obstacles to increasing production in crop-growing areas throughout the world. Salinity also impairs seed germination. Due to increasing salinity problems, in this study Festuca rubra seeds were treated with different concentrations of salt solutions with different sources of salt to study the response of this plant during seed germination and at the seedling stage. Germination percentage, coleoptile, radicle and seedling length, and seedling fresh weight (SFW) were determined under various salt stresses. Salt treatments included: NaCl, KNO3 and CaCl2 at 50, 100 and 150 mmol/L. An increase in salt concentration caused a decrease in germination. Lowest germination was observed by CaCl2 at 150 mmol/L while longest coleoptiles formed at 50 mmol/L CaCl2, i.e., longer than the control. An increase in KNO3 concentration from 0 to 50 mmol/L decreased radicle and seedling length more than the control (salt-free), or NaCl and CaCl2 treatments at equal concentrations. Highest SFW was observed in control seedlings and lowest SFW in seedlings grown in 150 mmol/L NaCl.
... In tomato and cucumber seeds, seed priming improves seed germination, seedling emergence and growth under saline conditions (Passam and Kakouriotis 1994;Cayuela et al. 1996). Under saline conditions, seed priming with KNO 3 was better than other priming treatments because summer squash (Cucurbita pepo) seedling fresh weight, root and shoot length increased (Shahi-Gharahlar et al. 2009). Farhoudi et al. (2007) suggested that canola seed priming with NaCl improved salinity tolerance in canola seed-ling because seed priming decreased seedling cell membrane damage. ...
... In many plants, germination and subsequent seedling growth can be inhibited by environmental stress such as salt stress (Okcu et al. 2005;Farooq and Azam 2006;Kaya et al. 2006;Shahi-Gharahlar et al. 2009). Priming may be helpful in reducing the risk of poor stand establishment under salt stress conditions (Nascimento 2003;Kaya et al. 2006). ...
This research was carried out in order to study the effect of seed priming on seedling emergence and seedling electrolyte leakage of snake melon (Cucumis melo var. flexuosus). Germination percentage, seedling dry weight (DW) and seedling growth decreased under salinity but mean germination time and electrolyte leakage increased. Under saline conditions seed priming by PEG solution decreased seedling DW but seed priming with KNO 3 was better than other priming treatments because seedling cell membrane stability, seedling DW, root and shoot length increased more than in other seed priming treatments.
... Under saline conditions, seed priming with KNO 3 (-1.27 MPa at EC=7 ds/m) was better than NaCl and PEG priming treatments because seedling fresh weight, root and shoot length increased in summer squash (Cucurbita pepo) (Shahi-Gharahlar et al. 2009). According to the results of this study, KNO 3 and NaCl at 100 mmol/L had the best effect on seed germination of F. rubra; however, when compared with the control, 50 mmol/L of any salt source, and 100 mmol/L of CaCl 2 showed no significant differences. ...
... Nascimento (2003) reported that muskmelon (Cucumis melo L.) seed priming with KNO 3 increased SFW under low temperature (at 17°C) stress in compared to non-primed seeds. Shahi-Gharahlar et al. (2009) suggested that, under saline conditions, seed priming with KNO 3 was better than other priming treatments (NaCl and PEG), because SFW, root and shoot length increased in summer squash (Cucurbita pepo) seedlings. Okcu et al. (2005) observed a remarkable decrease in SFW of pea (Pisum sativum L.) caused by a decrease in water potential when NaCl and PEG were used. ...
Salinity is one of the major obstacles to increasing production in crop growing areas throughout the world. Also, salinity impairs seed germination. Due to increasing salinity problems, in this study Festuca rubra seeds were treated with different concentrations of salt solutions with different sources of salt to study response of this plant in seed germination and seedling stage. Germination percentage, coleoptile length, radicle length, seedling length, and seedling fresh weight were determined under various salt stresses. Salt treatments included: NaCl, KNO3 and CaCl2 at 50, 100 and 150 mmol/L. results showed increasing of salt concentration caused a decrease in germination. Lowest germination was observed by CaCl2 at 150 mmol/L, while the highest coleoptile length was obtained by this salt source at 50 mmol/L. Between the salinity source, increase of KNO3 concentration from 0 to 50 mmol/L decreased radicle and seedling length as compared with control (salt free), NaCl and CaCl2 at equal level. Highest seedling fresh weight was obtained by seedling generated in control and lowest seedling fresh weight was obtained in NaCl at 150 mmol/L.
Key message
The potential of seed priming is still not fully exploited. Our limited knowledge of the molecular dynamics of seed pre-germinative metabolism is the main hindrance to more effective new-generation techniques.
Abstract
Climate change and other recent global crises are disrupting food security. To cope with the current demand for increased food, feed, and biofuel production, while preserving sustainability, continuous technological innovation should be provided to the agri-food sector. Seed priming, a pre-sowing technique used to increase seed vigor, has become a valuable tool due to its potential to enhance germination and stress resilience under changing environments. Successful priming protocols result from the ability to properly act on the seed pre-germinative metabolism and stimulate events that are crucial for seed quality. However, the technique still requires constant optimization, and researchers are committed to addressing some key open questions to overcome such drawbacks. In this review, an update of the current scientific and technical knowledge related to seed priming is provided. The rehydration–dehydration cycle associated with priming treatments can be described in terms of metabolic pathways that are triggered, modulated, or turned off, depending on the seed physiological stage. Understanding the ways seed priming affects, either positively or negatively, such metabolic pathways and impacts gene expression and protein/metabolite accumulation/depletion represents an essential step toward the identification of novel seed quality hallmarks. The need to expand the basic knowledge on the molecular mechanisms ruling the seed response to priming is underlined along with the strong potential of applied research on primed seeds as a source of seed quality hallmarks. This route will hasten the implementation of seed priming techniques needed to support sustainable agriculture systems.
This study was aimed to assess of priming effects on soluble proteins and
antioxidant enzymes of as a Nigella Sativa medicinal plant under different levels of
salinity and drought. Two greenhouse factorial experiments with three replicated
Randomized Complete Block Design were conducted. Treatments included five
levels of priming (potassium nitrate 1% and salicylic acid 0.2 mM in 6 hours,
potassium nitrate 3% and salicylic acid 0.5 mM in 12 hours and hydropriming in
24 hours) as main factor and four salinity levels (Control, 75, 125 and 175 mM
NaCl) and three levels of drought by osmotic potential of Control, -3 bar and -6 bar
by polyethylene glycol6000 as the second factor in each experiment. Results showed
that the interaction of salinity and drought stress on seed priming were significant
for all traits. Soluble protein concentration decreased along with increasing drought
and salinity stress and antioxidant enzymes activity, (which proline plays a
protective effect against stress), and MDA were increased. But, using priming, the
antioxidant enzymes activity and proline content were increased while MDA was
decreased. In this study, plants treated with integrated salicylic acid, potassium
nitrate and distilled water had usually higher antioxidant activity (catalase,
peroxidase and polyphenol oxidase) and soluble proteins in comparison with
untreated seedlings.
