Fig 6 - Ca2+-induced exocytosis in individual human neutrophils: High- and low-affinity granule populations and submaximal responses

$Fig. 6. Submaximal secretion at suboptimal stimulation. ( A and B ) Illustration of the time course of a calculated capacitance increase over time (C t ) following a first order reaction: C t ϭ C max *(1 – e –t/ τ ) Complete secretion corresponds to 100%. Variations in the speed, τ (A), or in the amplitude, C max (B), of the reaction are modelled. ( C and D ) Measured mean capacitance traces of 4–7 patched neutrophils per [Ca 2 ϩ ] pip condition. ( E and F ) Mean rate of exocytosis over the fraction of granules secreted ( ∆ C m /C total ; total ϭ 7.5 pF) derived from the mean traces in (C) and (D). The fraction of 0.3 (30%) of the total, as obtained with 10 μ M (E) and 60 μ M [Ca 2 ϩ ] pip (F), corresponds to the maximal release of phase 1, 2.25 pF.$

Submaximal secretion at suboptimal stimulation. ( A and B ) Illustration of the time course of a calculated capacitance increase over time (C t ) following a first order reaction: C t ϭ C max *(1 – e –t/ τ ) Complete secretion corresponds to 100%. Variations in the speed, τ (A), or in the amplitude, C max (B), of the reaction are modelled. ( C and D ) Measured mean capacitance traces of 4–7 patched neutrophils per [Ca 2 ϩ ] pip condition. ( E and F ) Mean rate of exocytosis over the fraction of granules secreted ( ∆ C m /C total ; total ϭ 7.5 pF) derived from the mean traces in (C) and (D). The fraction of 0.3 (30%) of the total, as obtained with 10 μ M (E) and 60 μ M [Ca 2 ϩ ] pip (F), corresponds to the maximal release of phase 1, 2.25 pF.

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Ca2+-induced exocytosis in individual human neutrophils: High- and low-affinity granule populations and submaximal responses - Scientific Figure on ResearchGate. Available from: https://www.researchgate.net/figure/Submaximal-secretion-at-suboptimal-stimulation-A-and-B-Illustration-of-the-time_fig6_13748367 [accessed 30 Jun, 2024]

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Fig. 6. Submaximal secretion at suboptimal stimulation. ( A and B ) Illustration of the time course of a calculated capacitance increase over time (C t ) following a first order reaction: C t ϭ C max *(1 – e –t/ τ ) Complete secretion corresponds to 100%. Variations in the speed, τ (A), or in the amplitude, C max (B), of the reaction are modelled. ( C and D ) Measured mean capacitance traces of 4–7 patched neutrophils per [Ca 2 ϩ ] pip condition. ( E and F ) Mean rate of exocytosis over the fraction of granules secreted ( ∆ C m /C total ; total ϭ 7.5 pF) derived from the mean traces in (C) and (D). The fraction of 0.3 (30%) of the total, as obtained with 10 μ M (E) and 60 μ M [Ca 2 ϩ ] pip (F), corresponds to the maximal release of phase 1, 2.25 pF.

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<a href="https://www.researchgate.net/figure/Submaximal-secretion-at-suboptimal-stimulation-A-and-B-Illustration-of-the-time_fig6_13748367"><img src="https://www.researchgate.net/profile/Daniel-Lew/publication/13748367/figure/fig6/AS:282488292298754@1444361865071/Submaximal-secretion-at-suboptimal-stimulation-A-and-B-Illustration-of-the-time.png" alt="Submaximal secretion at suboptimal stimulation. ( A and B ) Illustration of the time course of a calculated capacitance increase over time (C t ) following a first order reaction: C t ϭ C max *(1 – e –t/ τ ) Complete secretion corresponds to 100%. Variations in the speed, τ (A), or in the amplitude, C max (B), of the reaction are modelled. ( C and D ) Measured mean capacitance traces of 4–7 patched neutrophils per [Ca 2 ϩ ] pip condition. ( E and F ) Mean rate of exocytosis over the fraction of granules secreted ( ∆ C m /C total ; total ϭ 7.5 pF) derived from the mean traces in (C) and (D). The fraction of 0.3 (30%) of the total, as obtained with 10 μ M (E) and 60 μ M [Ca 2 ϩ ] pip (F), corresponds to the maximal release of phase 1, 2.25 pF."/></a>