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Structure de l'acide phytique. Acide phytique: inositol-1,2,3,4,5,6-hexaphosphate ou myo-inositol hexakisphosphate (InsP6)
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Le germe de soja est une matrice naturelle riche en substances biologiquement actives, ce qui lui confère un grand intérêt en terme de valorisation industrielle. L'objectif de ce travail a été de mettre en évidence les particularités de cette matière première par unecaractérisation approfondie de ses principaux métabolites, ciblés en fonction de le...
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Though not sold on commercial basis, mostly due to their flavour, shape, size and number of fingers per bunch, indigenous banana cultivars are said to contain functional and health beneficial properties which can be utilized on profiling of these cultivars.
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... The reducing power of an extract is associated with its antioxidant power. An increase in absorbance corresponds to an increase in the reducing power of the extracts tested 11 . The reaction is revealed by the transfer of the yellow color of iron (Fe 3+ ) to the green color of iron (Fe 2+ ). ...
The present study is devoted to evaluating the antioxidant activities and determining the inhibition of acetylcholinesterase for a plant used in traditional medicine to treat some skin diseases and in particular the scabies. The plant is Scabiosa atropurpurea L. Organic extracts were obtained by maceration with three solvents with increasing polarity: hexane, chloroform and methanol. The antioxidant activity was evaluated using three different methods: the DPPH• free radical scavenging activity, the ferric reducing antioxidant power (FRAP) assay and the carotene bleaching assay. All three methods showed that the methanolic extract had the highest antioxidant activity and was found to be the most efficient (IC50 = 27.5 μg/mL) against acetylcholinesterase. Consequently, a methanolic extract of Scabiosa atropurpurea L. could be exploited for its pharmacological applications in order to prevent induced diseases relying on oxidative stress.
... L'association de l'acide phytique avec la protéine lui confère des propriétés inhibitrices d'enzymes, en empêchant l'activité de l'alpha-amylase. Cela limite le métabolisme de l'amidon et diminue la glycémie (Hubert, 2006). Le trempage, seul ou combiné avec la cuisson, peut diminuer la teneur de ces FAN notamment les phytates (Rahelimandimby, 2011). ...
La carence en micronutriments comme le fer et le zinc est un problème de santé publique en République Démocratique du Congo. Les enfants de moins de cinq ans et les femmes enceintes sont des principales victimes. L'une des solutions face à cette carence est la consommation des cultures biofortifiées.
L'objectif de cette thèse est d'évaluer le comportement des génotypes de haricot commun biofortifiées, à sélectionner ceux qui sont intéressants du point de vue nutritionnel pour leur utilisation dans les programmes de sélection actuels et futurs.
... The reducing powers of the sample were determined from a calibration range established with vitamin C (0-125 µg/mL). An increase in the absorbance corresponded to an increase in the reducing power of the extracts tested (Hubert, 2006). ...
Crateva adansonii (CA) is traditionally used in the treatment of epilepsy and memory loss. This work aims to evaluate the antidepressant and anxiolytic activities of the dichloromethane/methanol extract of CA trunk bark in a chronic unpredictable stress-induced depression (UCMS) model in mice. After exposure of mice to UCMS for 42 days, anhedonia was assessed using the sucrose preference test, antidepressant effects by the forced swim and caudal suspension tests, anxiolytic effects by the light/dark compartment (LDB) and open arena (OF) tests. Oxidative stress parameters Malondialdehyde (MDA), Superoxide Dismutase (SOD), Catalase (CAT), and Reduced Glutathione (GSH) were assessed. The results showed that multiple administrations of C. adansonii extract (150 and 300 mg/kg, resulted in a significant increase from 38.5% to 64.9% (p<0.001) in sucrose intake and a decrease from 47 seconds to 14 seconds in the immobility time in the forced swim test compared to the UCMS group. The extract significantly (p<0.001) reversed the time spent in the dark box at 150 mg/kg, and the number of groomings at 150 and 300 mg/kg compared to the UCMS group in the LDB and OF test. There was also a significant (p<0.001) improvement in SOD, GSH, and a reversal of MDA. The extract of CA improved symptoms of depression and anxiety in mice treated with different dose. The effects observed would be due to the presence in the extract of polyphenols such as flavonoids. These effects would justify the use of this extract in traditional medicine.
... Among the legumes, soybeans are widely used in both human and animal nutrition. Soybeans contain about 40-45% protein, 18-22% lipids [7] , about 3-4% minerals [8] , and vitamins (A, B1, B2, B9, C and E) [9] . Fabiyi and Hamidu [10] reported that the quality of soy protein can be comparable to animal protein sources, such as: meat and milk. ...
Attiéké» is a fermented steamed cassava semolina produced mainly in Côte d'Ivoire. This food is an excellent source of energy, but it contains small amounts of protein and micronutrients. The objective of this study was to evaluate the nutritional and antioxidant potential of soy-enriched «attiéké». Eight formulations of soy-enriched «attiéké» were produced. Soybean flour was incorporated in proportions ranging from 0 to 12%. Micronutrients (minerals and vitamins A, B and C), phytonutrients, antinutrients and antioxidant activity were evaluated according to standard methods. The results showed that the contents of phosphorus, calcium, zinc, ß-carotene and vitamin B12 increased significantly with the amount of soy incorporated. The values changed from 60 to 260 mg/100g; 17.5 to 27.23 mg/100g; 1.52 to 3.08 mg/100g; 172.29 to 858.90 μg/100g and 1.18 to 1.28 μg/100g, respectively. Total polyphenols (136.51 to 337.61 mg/100g) and tannins (49.84 to 177.65 mg/100g) were positively influenced by soy level and fermentation. On the other hand, fermentation caused phytate (20.57 to 35.11 mg/100g) and oxalate (14 to 64.28 mg/100g) contents to decrease to acceptable levels. The molar ratios were favorable to the assimilation of minerals by the body. Total antioxidant activities ranged from 34.78% (plain «attiéké») to 66.64% (soybean flour). The consumption of soy enriched «attiéké» by providing micronutrients and phytochemicals essential for good health, could be beneficial to prevent and correct micronutrient deficiencies in malnourished people.
... Ferric reductive antioxidant activity (FRAP) is based on the ability of antioxidants to reduce ferric ion [Fe 3+ ] to ferrous [Fe 2+ ]. e mechanism is as an indicator of the donor activity of electrons, characteristic of the antioxidant action of the polyphenols [37]. e increase in absorbance in the reaction medium indicates an increase in the power of the tested extracts [38]. e phosphomolybdate method or total antioxidant capacity (TAC) is a technique based on the reduction of molybdenum Mo(VI) present in the form of molybdate ions MoO 2− 4 to molybdenum Mo(V) MoO + 2 in the presence of the extract to form a green complex of phosphate/Mo(V) at acid pH. ...
The purpose of this study is to determine the total content of phenols, flavonoids, and condensed tannins, as well as on the antioxidant activity of the extract, and their fractions were measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), phosphomolybdate reduction (or total antioxidant capacity), and cyclic voltammetry (CV). The hydromethanolic extract of Salvia officinalis showed the highest values of total phenolic (176 mgGAE/g of extract) and condensed tannins (162.53 mgEC/g of extract) from the Boulemane and Khenifra regions, respectively. The results showed that the best DPPH assay was found in the ethyl acetate fraction of Salvia officinalis leaves of the Boulemane region (IC50 = 0.002 mg/ml). For the ethyl acetate and butanolic fractions of Salvia officinalis leaves, those collected from different regions have a better reducing capacity (EC50 = 0.021 mg/ml, respectively). For the total antioxidant capacity, the best activity was found in the aqueous fraction of Salvia officinalis leaves of the Boulemane region (108 mgGAE/g of extract). By the cyclic voltammetry method, hydromethanolic extract of Salvia officinalis leaves from the Boulemane region showed an important result (288.8 mgGAE/g). There was a positive correlation between total phenol content (TPC), condensed tannin content (TCT), and total antioxidant capacity (TAC) (r = 0.932, r = 0.896, respectively). The main compounds that have been identified in the hydromethanolic extract of Salvia officinalis are ascorbic acid, gallic acid, 4-hydroxybenzoic acid, tannic acid, and rutin. Due to their antioxidant property, the leaf extracts from Salvia officinalis are used as natural preservative ingredients in food and/or pharmaceutical industries.
... The immense need for protein on a global level, the high cost of nitrogen fertilizers, or even their insufficiency in certain cases, pushed research to study the cultivation of protein legumes capable of using atmospheric nitrogen through a symbiotic association 2 . Among these plants, there is soybean, which is the main oil-produced and traded oil in the world 3 . Soyabean (Glycine max) is an excellent source of protein (35-40%), hence the seed is the richest in food value of all plant foods consumed in the world (Kure et al., 1998). ...
... As for staphylococci, the values counted varied between 7x10 1 and 10 2 CFU / ml. Finally, yeasts and molds were counted in milk obtained from the variety TGX 1951-3F (4x10 3 ...
Background: The economic situation in developing countries has made the supply of milk powder very difficult and unprofitable. Consequently, populations are increasingly resorting to substitute products including soy milk. The objective of this study is to highlight the microbiological, physico-chemical and organoleptic quality of soy milk produced from three varieties of soy acclimated in Benin. Materials and Methods: Soy milk was extracted from each variety after soaking and de-husking then heated to boiling before being filtered. Microbiological, physicochemical, nutritional and organoleptic analyzes were 0carried out by standard methods Results: The results showed that the samples were had satisfactory microbiological quality. The different milks had dry matter contents of 16.30, 17.81 and 16.07% respectively for the samples obtained from the varieties Jupiter, TGX 1987-62F and TGX 1951-3F. The pH of the milk samples varied from 9.16 to 9.54 and Protein content from 2.32% to 3.34%). Regarding their total ash content, this study shows that it varies from 0.10 to 0.18%. The calcium, magnesium and potassium content were higher (96.80 (mg / kg), 153.14 (mg / kg) and 774.99 (mg / kg)) respectively) for the variety TGX 1951-3F whereas the TGX 1987-62F variety samples had the lowest calcium and magnesium contents (52.91 and 111.11 mg / kg) respectively. Finally, the lowest potassium content (353.16 mg / kg) was obtained for the variety Jupiter. Organoleptic analyzes revealed that 60 to 73.33% of tasters estimated that the milks have a dirty-white color. None of the three milk samples had presented any deposit, according to 73.33 to 80% of the tasters. The three milks had very sweet tastes. Conclusion: The profiles obtained being relatively similar for the parameters evaluated, these different varieties can be used without distinction even if choices can be made depending on the nutrient sought.
... In addition, regular consumption of soy products would reduce the risk of prostate cancer; breast cancer; kidney disease and heart disease. This beneficial effect would be possible by bioactive compounds such as isoflavones that act as estrogens, antioxidants and / or enzyme regulators (Hubert, 2006). Formulations E and F, by adding 27.74 percent and 46.24 percent of orange-fleshed sweet potato flour, were obtained; 14.37 μg / 100 g (E) and 22.53 μg / 100 g (F) of beta carotene in a corn meal which was free of it. ...
The good use of food is one of the fundamental points of the food security of the populations especially in the developing countries. Therefore, for convincing results, the methods of strengthening nutritional knowledge by improving the culinary practices of vulnerable populations must take into account the dietary habits of the targets. The objective of the present study was to contribute to the consumption of the project crops to develop food formulations. In practice, eight (8) cornmeal formulas using soybeans and orange-fleshed sweet potatoes have been proposed and submitted to the grantees. The different proportions of ingredient to be mixed were obtained by the Pearson's Square method. Analysis of the sensory evaluation data was possible to the Statistical Package for Social Sciences (SPSS) software version 21 and the different results were presented in the form of radar graphs. The results showed that simultaneously flours and “kabato” accepted by the populations of the study area were formulations of: - E: 72.26 percent of maize flour and 27.74 percent of sweet potato flour - F: 53.76 percent of corn flour and 46.24 percent of sweet potato flour - G: 89.3 percent of composite flour (maize and sweet potato) and 10.7 percent of soya flour - H: 78.09 percent of composite flour (maize and sweet potato) and 21.91 percent of soya flour So, it can be envisaged to implement a strategy for a better vulgarization of these methods.
... L'absorbance du milieu réactionnel est déterminée à 700 nm. Une augmentation de l'absorbance correspond à une augmentation du pouvoir réducteur des extraits testés (Hubert, 2006). Protocole 1 ml de l'échantillon à différentes concentrations (0,1 ; 0,25 ; 0,75) mg/ml est mis dans un mélange de 2,5 ml d'une solution tampon phosphaté 0,2 M (pH=6,6) et 2,5 ml d'une solution de ferricyanure de potassium K3Fe(CN) 6 à 1%. . ...
... The absorbance was measured at 700 nm using a blank containing the constituents mentioned above except the extract which was replaced by distilled water. An increase in the absorbance corresponds to an increase in the reducing power of the extracts tested (Hubert, 2006). ...
Ficus carica L. is one of the first plants grown by humans, because of their consumption as much as a dry and fresh fruit. It is used in our traditional medicine for the healing of various diseases. The present study aims to evaluate the antioxidant activity of twelvedifferent extracts of three varieties of dried figs (Ficus carica L.).First, the extracts were investigated for their polyphenolic, flavonoidal and tannin content by using the Folin–Ciocalteu assay, the aluminium trichloride method and vanillin method, respectively and high-performance liquid chromatography (HPLC) for more qualitative and quantitative characterisation. Then, the antioxidant activity was performed by using three different methods the 2,2-diphenyl-2-picrylhydrazyl (DPPH), Ferric reducing antioxidant power (FRAP) and total antioxidant capacity(TAC).The results showed that the methanolic extract of Sidi Bendjebbar variety presented a higher TPC value (458 mgGAE /g) while the acetonic and aqueous extracts of El-Keurt presented a higher values of TFC and TTC (228.2 mgCE /g DMand 254.1 mgCE /g DM).In other hand, the methanol extract of El-Keurt varietyexhibited the highest antioxidant capacity with an IC50 of 0.078 mg/mL where the inhibition percentage was 90% approximatively¬.The antimicrobial activity of the extractsagainst various Gram positive and Gram negative bacteria was screened by the inhibition zone using the disc and diffusion assay, minimal inhibitionconcentration (MIC) by micro-well method allonwing to calculate the fractional inhibitory concentration (FIC)signaling a remarkable synergy between our extracts and gentamicin.The ethanolic extracts of the two varieties El-Keurt and Sidi Bendjebbar were the most effective extracts being able to inhibit the growth of the majority of the strains tested exclusively gram-positive bacteria.Our studysupported the use of these fruit as supplements for nutrient deficiencies and for combating diseases associated with oxydative damage or some microbial infection for better drug alternatives for treatmentof diseases.
Keywords: Ficus carica, polyphenols, antioxidant activity, antimicrobial activity, dried figs, MIC.
... The absorbance was measured at 700 nm using a blank containing the constituents mentioned above except the extract which was replaced by distilled water. An increase in the absorbance corresponds to an increase in the reducing power of the extracts tested (Hubert, 2006). ...
Ficus carica L. have always been known by their power to cure various diseases. This study aims to evaluate the antioxidant activity of twelve different extracts of three samples of dried figs. First, the extracts were tested for their polyphenolic, flavonoid and tannin contents by the Folin–Ciocalteu, the aluminium trichloride and vanillin methods and high-performance liquid chromatography (HPLC) for more characterisation. The antioxidant activity was performed by using thre different methods; the 2,2-diphenyl-2-picrylhydrazyl (DPPH), Ferric reducing antioxidant power (FRAP) and total antioxidant capacity (TAC). The results showed that the methanolic extract of Sidi Bendjebbar sample presented a higher TPC value (458 mg GAE /g) while the acetonic and aqueous extracts of El-Keurt presented a higher values of TFC and CTC. In other hand, the methanol extract of El-Keurt sample exhibited the highest antioxidant capacity with an IC50 of 0.078 mg/ml. The antimicrobial activity of the extracts against various Gram positive and Gram negative bacteria was screened by the inhibition zone using the disc and diffusion assay, minimal inhibition concentration (MIC) by micro-well method allonwing to calculate the fractional inhibitory concentration (FIC). The ethanolic extracts of the two samples El-Keurt and Sidi Bendjebbar were the most effective extracts being able to inhibit the growth of the majority of the strains tested. Our study supported the use of these fruit as supplements for nutrient deficiencies and for combating diseases associated with oxydative damage or some microbial infection for better drug alternatives.
