FIGURE 6 - uploaded by Huang Zhang
Content may be subject to copyright.
Sporulation of Parafuscosporella pyriformis on PDA. a-i Conidia and conidiogenous cells from culture. Scale bars: a-i = 10 μm. Vanakripa minutiellipsoidea A. Pinnoi. Nova Hedwigia 77: 217 (2003) (FIGURE 7) Index Fungorum number: IF489337 Saprobic on submerged wood. Sexual morph: undetermined. Asexual morph: Colonies on the natural substratum forming scattered, granular, black sporodochia. Mycelium mostly immersed in the substratum. Conidiophores micronematous, hyaline. Conidiogenous cells monoblastic, cylindrical, 4-5.5 μm wide. Separating cells cylindrical, clavate to vermiform, 27-45 × 5-13 μm ( = 39 × 9 µm, n = 20), hyaline. Conidia ellipsoid to broadly clavate, dark brown to black, aseptate, 18-29 × 13-20 µm ( = 25 × 17 µm, n = 30).
Source publication
A collection of lignicolous freshwater fungi in the Greater Mekong Subregion resulted in four interesting dematiaceous hyphomycetes with similar morphology. The taxa had sporodochial colonies, micronematous to semi-macronematous conidiophores and subglobose, ellipsoidal to broadly pyriform, 0–1-septate, dark brown to black conidia. Based on morphol...
Similar publications
Gohteikhimyces , a new genus of dematiaceous hyphomycete occurring on decaying wood submerged in a freshwater stream of Taiwan, is proposed based on morphological and molecular data. It produces effuse, black, and somewhat glistening colonies on the surface of natural substratum, which mainly composed of solitary, dry, dark, cymbiform or ellipsoida...
Over the past two decades, numerous novel species have been identified within Dictyosporiaceae, primarily in Dictyocheirospora and Dictyosporium. A recent monograph has revealed that these two genera exhibit a distinct preference for freshwater habitats, particularly in southern China. However, further investigation into the distribution and divers...
Freshwater fungi play an indispensable role in the ecosystem and have great research value. Based on morphological and phylogenetic analyses of a concatenated dataset of ITS, LSU and SSU sequences, a new species, Phaeoisaria laianensis , was introduced as a freshwater hyphomycete from Anhui Province, China.
Phaeoisaria laianensis was morphologicall...
Background Freshwater fungi play an indispensable role in the ecosystem and have great research value. Based on morphological and phylogenetic analyses of a concatenated dataset of ITS, LSU and SSU sequences, a new species, Phaeoisaria laianensis , was introduced as a freshwater hyphomycete from Anhui Province, China. New information Phaeoisaria la...
During the investigation of lignicolous freshwater fungi in the Tibetan Plateau habitat, fifteen collections were isolated from submerged decaying wood. Fungal characteristics are commonly found as punctiform or powdery colonies with dark pigmented and muriform conidia. Multigene phylogenetic analyses of combined ITS, LSU, SSU and TEF DNA sequences...
Citations
... Thus, they play an important role in freshwater environment (Koske & Duncan 1973, Zare-Maivan & Shearer 1988, Duarte et al. 2013. Studies on lignicolous freshwater fungi have mainly concentrated in tropical, subtropical and temperate regions , 2018, Monkai et al. 2020, Yang et al. 2020, Dong et al. 2021. Yunnan Province is one of the richest biodiversity hotspots in China, containing abundant resources of lignicolous freshwater fungi (Shen et al. 2022). ...
Diversity of lignicolous freshwater fungi in Yuanjiang (Red River) basin, Yunnan, China is currently being studied. Three fresh collections of Papulosaceae were collected. Wongia suae sp. nov. and W. fusiformis were identified based on morphology and phylogenetic analysis of combined ITS, LSU, rpb2, SSU and tef1-α sequence data. The detailed descriptions and illustrations of these two species are provided, as well as the morphological comparison with similar taxa.
... Parafuscosporella is characterized by semi-macronematous, mostly moniliform, hyaline conidiophores, monoblastic, hyaline, smooth-walled conidiogenous cells and acrogenous, ellipsoidal to broadly obpyriform, septate, dark brown to black conidia, sometimes with a pale brown basal cell or a small protuberance (Yang et al. 2016, Boonyuen et al. 2016. So far, eight species have been reported in the genus, including P. aquatica, P. ellipsoconidiogena, P. garethii, P. moniliformis, P. mucosa, P. nilotica, P. obovata and P. pyriformis (Boonyuen et al. 2016, Yang et al. 2016, 2020, Boonmee et al. 2021. All of them are from Thailand, except P. nilotica, which was collected in Egypt. ...
... The sequence data for LSU, ITS and rpb2 generated in this study were subjected to BLASTn searches in GenBank database for the preliminary identification of the fungus (www.ncbi.nlm.nih.gov/blast/). A dataset composed of LSU, SSU, ITS and rpb2 sequences was then constructed using the newly generated sequence data in the present study along with taxa downloaded from GenBank and from recent relevant publications (Yang et al. 2020, Boonyuen et al. 2021. GenBank accession numbers of the fungal strains used in this study are shown in TABLE 2. The dataset was firstly aligned using MAFFTv.7 (Katoh & Standley 2013) on the online server (http://maffTh.cbrc.jp/alignment/ ...
In this study, a novel lignicolous freshwater species, Parafuscosporella xishuangbannaensis, which was collected from Manfeilong reservoir in Xishuangbanna, Yunnan Province, China, is described and illustrated. The new species was established based on morphological characteristics and multi-locus phylogenetic analyses of combined LSU, SSU, ITS and rpb2 sequence data. Parafuscosporella xishuangbannaensis is characterized by reduced conidiophores, holoblastic, monoblastic, integrated, terminal, hyaline, cylindrical conidiogenous cells, and acrogenous, obovoid to subglobose, brown to dark brown conidia with a septum near the base and a paler, wedge-shaped basal cell. Parafuscosporella xishuangbannaensis differs from its phylogenetically close sister taxon P. pyriformis in lacking cylindrical or moniliform conidiophores.
... (Liu et al. 2019a;Luo et al. 2019;Yuan et al. 2020). However, based on molecular DNA data, Yang et al. (2020) confirmed the systematic placement of a Vanakripa species within the Conioscypha clade. ...
... They recognized these cells as the separating cells bearing conidia, referring to similar features in Beltrania, Beltraniopsis and Beltraniella (Pirozynski 1963). Molecular DNA data is only available for V. minutiellipsoidea and V. chiangmaiense (Vu et al. 2019;Yang et al. 2020;Phukhamsakda et al. 2022). In our phylogenetic analysis (Fig. 53), they positioned within the Conioscypha clade consistent with the result in Yang et al. (2020) and Phukhamsakda et al. (2022). ...
... Molecular DNA data is only available for V. minutiellipsoidea and V. chiangmaiense (Vu et al. 2019;Yang et al. 2020;Phukhamsakda et al. 2022). In our phylogenetic analysis (Fig. 53), they positioned within the Conioscypha clade consistent with the result in Yang et al. (2020) and Phukhamsakda et al. (2022). Vanakripa chiangmaiensis X.G. ...
Freshwater fungi comprises a highly diverse group of organisms occurring in freshwater habitats throughout the world. During a survey of freshwater fungi on submerged wood in streams and lakes, a wide range of sexual and asexual species were collected mainly from karst regions in China and Thailand. Phylogenetic inferences using partial gene regions of LSU, ITS, SSU, TEF1α, and RPB2 sequences revealed that most of these fungi belonged to Dothideomycetes and Sordariomycetes and a few were related to Eurotiomycetes. Based on the morphology and multi-gene phylogeny, we introduce four new genera, viz. Aquabispora, Neocirrenalia, Ocellisimilis and Uvarisporella, and 47 new species, viz. Acrodictys chishuiensis, A. effusa, A. pyriformis, Actinocladium aquaticum, Annulatascus tratensis, Aquabispora setosa, Aqualignicola setosa, Aquimassariosphaeria vermiformis, Ceratosphaeria flava, Chaetosphaeria polygonalis, Conlarium muriforme, Digitodesmium chishuiense, Ellisembia aquirostrata, Fuscosporella atrobrunnea, Halobyssothecium aquifusiforme, H. caohaiense, Hongkongmyces aquisetosus, Kirschsteiniothelia dushanensis, Monilochaetes alsophilae, Mycoenterolobium macrosporum, Myrmecridium splendidum, Neohelicascus griseoflavus, Neohelicomyces denticulatus, Neohelicosporium fluviatile, Neokalmusia aquibrunnea, Neomassariosphaeria aquimucosa, Neomyrmecridium naviculare, Neospadicoides biseptata, Ocellisimilis clavata, Ophioceras thailandense, Paragaeumannomyces aquaticus, Phialoturbella aquilunata, Pleurohelicosporium hyalinum, Pseudodactylaria denticulata, P. longidenticulata, P. uniseptata, Pseudohalonectria aurantiaca, Rhamphoriopsis aquimicrospora, Setoseptoria bambusae, Shrungabeeja fluviatilis, Sporidesmium tratense, S. versicolor, Sporoschisma atroviride, Stanjehughesia aquatica, Thysanorea amniculi, Uvarisporella aquatica and Xylolentia aseptata, with an illustrated account, discussion of their taxonomic placement and comparison with morphological similar taxa. Seven new combinations are introduced, viz. Aquabispora grandispora (≡ Boerlagiomyces grandisporus), A. websteri (≡ Boerlagiomyces websteri), Ceratosphaeria suthepensis (≡ Pseudohalonectria suthepensis), Gamsomyces aquaticus (≡ Pseudobactrodesmium aquaticum), G. malabaricus (≡ Gangliostilbe malabarica), Neocirrenalia nigrospora (≡ Cirrenalia nigrospora), and Rhamphoriopsis glauca (≡ Chloridium glaucum). Ten new geographical records are reported in China and Thailand and nine species are first reported from freshwater habitats. Reference specimens are provided for Diplocladiella scalaroides and Neocirrenalia nigrospora (≡ Cirrenalia nigrospora). Systematic placement of the previously introduced genera Actinocladium, Aqualignicola, and Diplocladiella is first elucidated based on the reference specimens and new collections. Species recollected from China and Thailand are also described and illustrated. The overall trees of freshwater Dothideomycetes and Sordariomycetes collected in this study are provided respectively and genera or family/order trees are constructed for selected taxa.
... Parafuscosporella nilotica has much smaller conidial sizes than all species; 18-22 × 11-15 μm vs 20-29 × 13-19 μm for P. aquatica; (37.5-)40-47.5 × (25-)27.5-42.5 µm for P. garethii; 28-37 × 14-21 μm for P. moniliformis; 26.5-36 × 12-26 μm for P. mucosa and 23-30 × 16-26 μm for P. pyriformis Yang et al. 2016Yang et al. , 2020. ...
This article is the 13th contribution in the Fungal Diversity Notes series, wherein 125 taxa from four phyla, ten classes, 31 orders, 69 families, 92 genera and three genera incertae sedis are treated, demonstrating worldwide and geographic distribution. Fungal taxa described and illustrated in the present study include three new genera, 69 new species, one new combination, one reference specimen and 51 new records on new hosts and new geographical distributions. Three new genera, Cylindrotorula (Torulaceae), Scolecoleotia (Leotiales genus incertae sedis) and Xenovaginatispora (Lindomycetaceae) are introduced based on distinct phylogenetic lineages and unique morphologies. Newly described species are Aspergillus lannaensis, Cercophora dulciaquae, Cladophialophora aquatica, Coprinellus punjabensis, Cortinarius alutarius, C. mammillatus, C. quercofocculosus, Coryneum fagi, Cruentomycena uttarakhandina, Cryptocoryneum rosae, Cyathus uniperidiolus, Cylindrotorula indica, Diaporthe chamaeropicola, Didymella azollae, Diplodia alanphillipsii, Dothiora coronicola, Efbula rodriguezarmasiae, Erysiphe salicicola, Fusarium queenslandicum, Geastrum gorgonicum, G. hansagiense, Helicosporium sexualis, Helminthosporium chiangraiensis, Hongkongmyces kokensis, Hydrophilomyces hydraenae, Hygrocybe boertmannii, Hyphoderma australosetigerum, Hyphodontia yunnanensis, Khaleijomyces umikazeana, Laboulbenia divisa, Laboulbenia triarthronis, Laccaria populina, Lactarius pallidozonarius, Lepidosphaeria strobelii, Longipedicellata megafusiformis, Lophiotrema lincangensis, Marasmius benghalensis, M. jinfoshanensis, M. subtropicus, Mariannaea camelliae, Melanographium smilaxii, Microbotryum polycnemoides, Mimeomyces digitatus, Minutisphaera thailandensis, Mortierella solitaria, Mucor harpali, Nigrograna jinghongensis, Odontia huanrenensis, O. parvispina, Paraconiothyrium ajrekarii, Parafuscosporella niloticus, Phaeocytostroma yomensis, Phaeoisaria synnematicus, Phanerochaete hainanensis, Pleopunctum thailandicum, Pleurotheciella dimorphospora, Pseudochaetosphaeronema chiangraiense, Pseudodactylaria albicolonia, Rhexoacrodictys nigrospora, Russula paravioleipes, Scolecoleotia eriocamporesi, Seriascoma honghense, Synandromyces makranczyi, Thyridaria aureobrunnea, Torula lancangjiangensis, Tubeufa longihelicospora, Wicklowia fusiformispora, Xenovaginatispora phichaiensis and Xylaria apiospora. One new combination, Pseudobactrodesmium stilboideus is proposed. A reference specimen of Comoclathris permunda is designated. New host or distribution records are provided for Acrocalymma fci, Aliquandostipite khaoyaiensis, Camarosporidiella laburni, Canalisporium caribense, Chaetoscutula juniperi, Chlorophyllum demangei, C. globosum, C. hortense, Cladophialophora abundans, Dendryphion hydei, Diaporthe foeniculina, D. pseudophoenicicola, D. pyracanthae, Dictyosporium pandanicola, Dyfrolomyces distoseptatus, Ernakulamia tanakae, Eutypa favovirens, E. lata, Favolus septatus, Fusarium atrovinosum, F. clavum, Helicosporium luteosporum, Hermatomyces nabanheensis, Hermatomyces sphaericoides, Longipedicellata aquatica, Lophiostoma caudata, L. clematidisvitalbae, Lophiotrema hydei, L. neoarundinaria, Marasmiellus palmivorus, Megacapitula villosa, Micropsalliota globocystis, M. gracilis, Montagnula thailandica, Neohelicosporium irregulare, N. parisporum, Paradictyoarthrinium difractum, Phaeoisaria aquatica, Poaceascoma taiwanense, Saproamanita manicata, Spegazzinia camelliae, Submersispora variabilis, Thyronectria caudata, T. mackenziei, Tubeufa chiangmaiensis, T. roseohelicospora, Vaginatispora nypae, Wicklowia submersa, Xanthagaricus necopinatus and Xylaria haemorrhoidalis. The data presented herein are based on morphological examination of fresh specimens, coupled with analysis of phylogenetic sequence data to better integrate taxa into appropriate taxonomic ranks and infer their evolutionary relationships.
... Based on morphological and molecular data, the type species without sexual morph, P. moniliformis Jing Yang, Bhat & K.D. Hyde, was described on dead and decaying submerged wood in Thailand. To date, five accepted species, namely, P. aquatica H. Yang [1][2][3][4]. In this study, we describe P. ellipsoconidiogena and P. obovata as the sixth and seventh species in the genus, respectively, collected from a waterfall in Chiang Mai Zoo, Chiang Mai Province, Thailand. ...
... Note: Parafuscosporella obovata is clearly distinct from other members of the genus based on molecular data. Morphologically, P. obovata is most similar to P. aquatica and P. moniliformis in natural substratum colonies without a jelly-like covering, moniliform conidiophores, globose to subglobose conidiogenous cells and dark-pigmented conidia with a transverse septum [1,4]. However, P. moniliformis differs from P. obovata in having larger (5.5-36 × 5-21 μm), ellipsoidal or clavate conidiogenous cells [1]. ...
... × 13-32.5 μm) conidia. Parafuscosporella obovata differs from P. aquatica in having broadly obovoid to subglobose and larger conidia, while the smaller conidia of P. aquatica (20-29 × 13-19 μm) are ellipsoidal to obovoid [4]. ...
Asexual morphs of freshwater fungi have been mostly reported from tropical and subtropical regions. From our ongoing investigation of the diversity and taxonomy of freshwater mi-crofungi in Thailand, a country with rich natural resources and diverse ecosystems, Parafuscosporella ellipsoconidiogena sp. nov. and P. obovata sp. nov., collected from decaying submerged twigs at Phalad Waterfall in a conserved forest in Chiang Mai Zoo, Chiang Mai Province, northern Thailand, are proposed. DNA phylogenies based on a combination of ITS and LSU datasets support the placement of these species in Parafuscosporella (Fuscosporellaceae, Fuscosporellales, Sordariomycetes), and these two novel species differ from known species in terms of morphology. Detailed descriptions , illustrations and a key to Parafuscosporella species are provided, as well as comparisons with other accepted Parafuscosporella species.
... Diabolocovidia has micronematous rather than synnematous conidiophores, blastic rather than tretic conidiogenous cells, and ellipsoid to obovoid, aseptate conidia [49]. The phenomenon that Diabolocovidia mixes with synnematous and tretic genera like Didymobotryum and Vamsapriya reminds us of an example that Vanakripa with blastic conidiogenous resides in the phialidic genus Conioscypha [65]. These probably indicate the polyphyletic nature of some hyphomycetous groups. ...
Phylogenetic analyses of combined LSU, rpb2, tub2 and ITS sequence data of representative Xylariales taxa indicated that Diabolocovidia, Didymobotryum and Vamsapriya cluster together and form a distinct clade in Xylariales. Morphological comparison also shows their distinctiveness from other families of Xylariales. Therefore, we introduce it as a novel family, Vamsapriyaceae. Based on morphological characteristics, Podosporium and Tretophragmia, which were previously classified in Ascomycota genera incertae sedis, are now included in the Vamsapriyaceae. In addition, three Vamsapriya species, V. chiangmaiensis sp. nov, V. uniseptata sp. nov, and V. indica are described and illustrated in this paper.
... Forty new species of freshwater Sordariomycetes were discovered in Thailand over the past five years, and taxa were members of Amphisphaeriales [10], Annulatascales [16], Chaetosphaeriales [10,97], Diaporthomycetidae genera incertae sedis ([59,117,118], Distoseptisporales [10,16,97,119,120], Magnaporthales [10], Microascales [10,121], Pleurotheciales [10,27,101,118], Pseudodactylariales [27], Savoryellales [27], Sporidesmiales [103], and Xylariales [10]. Three novel Sordariomycetes (Neoxylomyces multiseptatus, Phaeoacremonium thailandense, and Rattania aquatica) are additions to these species thriving in freshwater habitats in Thailand. ...
An investigation of freshwater fungi in Thailand resulted in the collection of one new monotypic genus, Neoxylomyces, and a novel species each in Camposporium, Brunneofusispora, Rattania, Neoxylomyces, and Phaeoacremonium.Camposporium dulciaquae resembles C. septatum in conidial morphology and number of septa but differs in conidial sizes. Brunneofusispora hyalina is similar to B. sinensis in conidiogenesis and conidial shape but differs in the sizes of conidiomata and conidiogenous cells. Rattania aquatica is the second species in Rattania, while Phaeoacremonium thailandense is the third species recorded from freshwater habitats. A new genus, Neoxylomyces, typified by N. multiseptatus, is similar to Xylomyces giganteus, but differs in the number of septa, chlamydospore measurements, and absence of a mucilaginous coating around the chlamydospores. These novel taxa form an independent lineage distinct from other species based on multi-loci phylogenetic analyses. Descriptions, illustrations, and notes are provided for each taxon. These new freshwater ascomycetes add to the increasing number of fungi known from Thailand and it is now evident that there are numerous novel taxa awaiting to be described as new freshwater habitats are explored. An update of newly discovered taxa in the widely studied freshwater habitats of Thailand over the last five years is also provided.
Vanakripa oblonga sp. nov. and V. taiwanensis sp. nov. are described from decaying wood submerged in freshwater streams of Taoyuan District, Kaohsiung City, Taiwan. The phylogenetic relationship of Vanakripa species is inferred from concatenated sequences of their nuc rDNA that were aligned with sequences of related fungal lineages including Conioscyphales, Fuscosporellales, Pleurotheciales, and Savoryellales. The two Vanakripa species from Taiwan clustered within the group comprising Parafuscosporella species with good support. Morphologically, all Parafuscosporella species and Vanakripa gigaspora, the type species of Vanakripa, produce more or less obovoid or pyriform, apiosporous, versicolored conidia with a paler basal cell. Based on the principle of nomenclatural priority, all nine Parafuscoporella species are transferred to Vanakripa. The generic circumscription of Vanakripa is elaborated. Two previously described Vanakripa species with unicellular and ellipsoidal conidia, namely V. chiangmaiensis and V. minutiellipsoidea, do not cluster with Parafuscosporella species but with Conioscypha species. These two Vanakripa species, together with the other seven Vanakripa species having unicellular and ellipsoidal conidia and morphologically differ from V. gigaspora, are excluded from Vanakripa sensu stricto. With the present revision, there are 13 accepted Vanakripa species. Species are morphologically identifiable with the help of a provided dichotomous key. The term “separating cells” used to describe the hyaline appendages at the base of conidia and the misused adjective “obpyriform”, used to describe the conidial shape of several Parafuscosporella and Vanakripa species, is discussed.
A survey of lignicolous freshwater fungi in Thailand resulted in the discovery of one new species in Parafuscosporella herein described as Parafuscosporella lignicola. It is characterized by sporodochial colonies, moniliform or ellipsoidal conidiophores, ellipsoidal or cylindrical conidiogenous cells, irregular ellipsoidal and dark brown conidia. Phylogenetic analysis based on the combined LSU, SSU and ITS sequence data support its placement in Parafuscosporella. The species formed distinct clade from the type species and represents as ninth species in Parafuscosporella. The results of the pairwise
Research into freshwater fungi has generated a wealth of information over the past decades with various published articles, i.e., reviews, books, and monographs. With the advancement of
methodologies used in freshwater fungal research, and numerous mycologists working on this ecological group, our knowledge progress and understanding of freshwater fungi, including novel
discoveries and new insights in the ecology of freshwater fungi, has advanced. With this enormous progress, it is timely that an updated account of freshwater fungi be compiled in one volume. Thus, this account is published to give a comprehensive overview of the different facets of freshwater fungal biology. It includes an updated classification scheme based on the latest taxonomic and phylogenetic analysis of freshwater fungal taxa, including their evolutionary history. The biology, diversity, and geographical distribution of higher and basal freshwater fungi are also discussed in the entries. A section on dispersal and adaptation of filamentous freshwater fungi is included in the present work. The ecological importance and role of fungi in the breakdown of wood in freshwater habitats, including their physiology, are discussed in detail. The biotechnological potential of freshwater fungi as producers of bioactive metabolites are reviewed, with methodologies in antimicrobial drug discovery. The present volume also provides an overview of different high throughput sequencing (HTS) platforms for freshwater fungal research highlighting their advantages and challenges, including recent studies of HTS in identification and quantification of fungal communities in freshwater habitats. The present volume also identifies the knowledge gaps and direction of future research in freshwater fungi.
