Figure 2 - available via license: Creative Commons Attribution 4.0 International
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Specific activities (U mg -1 ) based on initial reaction rates of HheG and HheG-682 in epoxide ring opening of epoxides epichlorohydrin (1), phenylglycidylether (3), styrene oxide (5), cyclohexene oxide (7), (+)-cis/trans-limonene oxide (9) and trans-1-phenylpropylene oxide (11) determined with a
Source publication
Biochemical characterization of HheG-682, a new homolog of halohydrin dehalogenase HheG sharing 46% sequence identity, is presented.
Context in source publication
Context 1
... Reactions were performed at 30°C using 10 mM epoxide, 20 mM azide and 100 µg mL -1 purified enzyme. As expected, both HheG and HheG-682 displayed low specific activity with epoxide 1 (Figure 2), while for all other tested epoxides the specific activities of HheG-682 were significantly higher compared to HheG. The highest (13.6-fold) increase in specific activity was obtained with epoxide 11. ...
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