Table 5 - uploaded by Santosh Shelke
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NH-Hydrochloride (NH) is a selective 5-HT 1 agonist developed for the acute treatment of migraine which acts by stimulating constriction of dilated cranial arteries and by inhibiting the release of neurogenic inflammatory mediators. A simple, sensitive, precise, accurate and economical UV-Spectrophotometric method has been developed for the determi...
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Context 1
... maximum wavelength observed was at 222 and 283 nm (table 5), where the wavelength of 283nm was consid- ered for the analytical method development. The value of molar extinction coefficient and sandell's sensitivity for EH estimated was 6.743 x 10 3 L/mol/cm and 0.497 μg/cm 2 re- spectively, as mentioned All the discussed parameters are listed in the table 5. ...
Context 2
... quantitation limit is a parameter of quantitative assays for low levels of compounds in sample matrices which is basically used for the determination of impurities and/or degradation products. The valve of LOD (0.323) and LOQ (0.979) are determined by using standard deviation of the response and slope approach as defined in International Conference on Harmonization (ICH) guidelines [12] and are given in Table 5.Precision (Table 2), recovery data (Ta- ble 3) and assay (Table 4) confirms the reproducibility and accuracy of the method. ...
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A simple, rapid and precise spectrophotometric method has been developed for simultaneous estimation of Metformin and Glipizide. The method involved estimation of Metformin and Glipizide by simultaneous equation at 272nm and 232nm respectively in their solution in water. This method was validated with respect to linearity, accuracy, precision, LOD...
Citations
... In the available formulations, the treatment is preferred by the oral route where these conditions may lead to unpredictable drug release from the gastrointestinal tract resulting into unproductive treatment [18]. Oral route is considered the most suitable route of drug administration; however, this route of drug administration is sometimes ineffective due to the poor bioavailability, first pass metabolism, drug solubility, and absorption issues; thus, there is an obvious need for alternative but novel drug delivery systems [2,3,45,46,55]. Target drug delivery system which can release the drug to the targeted site (brain and vasculature) can be the benchmark for the efficient treatment for migraine attacks. ...
... Spectrophotometric method was used to determine the drug content of the ethosomal gels. Dilutions were prepared in the linear range of 1-10 μg/ml and were analyzed for the absorbance at the wavelength of 283 nm against blank [45,46]. The viscosity of the gel was assessed at 10 ± 2°C temperature using Brookfield viscometer (DV3T Rheometer, USA). ...
... Minimum weight required to separate the tissues from the surface of individual formulation is used to determine the potential (mucoadhesive strength) of gel formulation. Mucoadhesive strength is calculated using the following formula [45,46]: ...
Migraine is the chronic neurological disorder identified by recurrent moderate to severe headache. Research was highlighted to formulate target drug delivery to treat migraine via the olfactory lobe through the intranasal route using ethosomal thermoreversible gel. Ethosomes were prepared by thin film hydration method using 32 factorial design. The prepared ethosomes were evaluated for percent drug loading, vesicle size, zeta potential, and polydispersity index, whereas nanoethosomal in situ gels were assessed for their pH, viscosity, mucoadhesive strength, and in vitro drug release. Ex vivo drug permeation was evaluated using nasal mucosa of sheep. The data produced from the experimental design indicated NE6 (soya lecithin 3%:ethanol 50%) as the optimized ethosome formulation, whereas NG3 (carbol 934-0.3%) and NG6 (PVP K30-0.3%) were recorded as the optimized in situ gel formulations. In vitro and ex vivo drug release demonstrated almost 100% release after 24 h for optimized formulations. In conclusion, drug-loaded in situ mucoadhesive gels could release the drug for longer duration of time which can improve the bioavailability providing new dimension to the treatment of migraine.
... In the available formulations, the treatment is preferred by the oral route where these conditions may lead to unpredictable drug release from the gastrointestinal tract resulting into unproductive treatment [18]. Oral route is considered the most suitable route of drug administration; however, this route of drug administration is sometimes ineffective due to the poor bioavailability, first pass metabolism, drug solubility, and absorption issues; thus, there is an obvious need for alternative but novel drug delivery systems [2,3,45,46,55]. Target drug delivery system which can release the drug to the targeted site (brain and vasculature) can be the benchmark for the efficient treatment for migraine attacks. ...
... Spectrophotometric method was used to determine the drug content of the ethosomal gels. Dilutions were prepared in the linear range of 1-10 μg/ml and were analyzed for the absorbance at the wavelength of 283 nm against blank [45,46]. The viscosity of the gel was assessed at 10 ± 2°C temperature using Brookfield viscometer (DV3T Rheometer, USA). ...
... Minimum weight required to separate the tissues from the surface of individual formulation is used to determine the potential (mucoadhesive strength) of gel formulation. Mucoadhesive strength is calculated using the following formula [45,46]: ...
... A literature survey revealed that ESC is official in Indian pharmacopoeia and ETZ is official only in Japanese Pharmacopoeia [7, 8]. The literature survey revealed that some Spectrophotometric [9], HPTLC [10][11][12], colorimetric [13] HPLC [14][15], fluorimetry [16], LC-MS [17][18][19], CE [20] and TLC [21] methods used for estimation of ESC either individually or in combination with other drugs. Literature survey also reports few HPLC [22], HPTLC [23], LC-MS [24] and GC-MS methods for estimation of ETI individually or in combination with other drugs. ...
The present work describes a simple, selective, rapid, accurate and economic method which has been developed and validated for simultaneous estimation of Escitalopram oxalate (ESC) and Etizolam (ETZ) in tablet dosage form by reverse phase Ultra Performance Liquid Chromatography (RP-UPLC) with PDA detector. The chromatographic separation was carried out on a column Symmetry C 18 (4.6×50mm, 5microm) by using mobile phase mixture of buffer (pH was adjusted at3 by using ortho phosphoric acid) and acetonitrile at ratio of 85:15 v/v at a flow rate 0.5 ml/min. The PDA detection made at 230 nm. The retention time is for Escitalopram oxalate and Etizolam was found 0.752 ±0.002 min & 2.612 ±0.003 min respectively with a run time of 4 minute and resolution of 22.41. Calibration plots were linear over the concentration ranges 80-120 μg/ml and 8-12 μg/ml for ESC and ETZ respectively. The developed method was validated in terms of linearity, accuracy, precision, limit of detection, limit of quantitation and robustness as per ICH guidelines given for analytical method validation and the method was also found to be simple, sensitive, accurate and precise.
