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Serum level of testosterone. Orchiectomy and exogenous testosterone administration were effective in promoting sub and supraphysiological testosterone levels, while animals in control group maintained physiological concentration of the hormone as observed on day 22. *p < 0.05 when compared to the control group on day 22, **p < 0.05 when compared to the OCX group on day 22; #p < 0.05 when compared to the respective group on day 1. Data are shown as mean ± SEM
Source publication
Objective The aim of this study was to evaluate the effects of both testosterone depletion and supraphysiological testosterone
supplementation in the early phase of an animal cutaneous wound healing model in comparison with the physiological
hormonal condition.
Material and Methods Forty rats were distributed into the following four groups: Control...
Citations
... 49,50 Systemic factors encompass a range of issues such as smoking, obesity, malnutrition, impaired mobility, diabetes, and gender, with hormones playing a significant role; estrogens positively and testosterone negatively affecting wound healing. 51,52 Clinical, Cosmetic and Investigational Dermatology 2024:17 https://doi.org/10.2147/CCID.S467442 ...
Curcumin, derived from Curcuma longa (turmeric), exhibits significant potential in dermatology, addressing conditions like atopic dermatitis, psoriasis, chronic wounds, skin cancer, and infections through its anti-inflammatory, antioxidant, anticancer, and antimicrobial properties. This review synthesizes evidence on curcumin’s mechanisms, including modulation of immune responses and promotion of wound healing, showcasing its efficacy in reducing inflammation, cytokine levels, and enhancing skin barrier functions. Studies highlight curcumin’s ability to selectively target tumor cells, suggesting a multifaceted approach to cancer therapy with minimal side effects. Despite promising therapeutic benefits, challenges remain in bioavailability, potency, and targeted delivery, underscoring the need for further research to optimize dosages, delivery methods, and assess long-term safety. The integration of curcumin into dermatological practice requires a balanced consideration of evidence-based efficacy and safety. Curcumin’s comprehensive utility in dermatology, coupled with the necessity for advanced scientific exploration, emphasizes the importance of combining traditional knowledge with contemporary research to improve patient care in dermatology. This approach could significantly enhance outcomes for individuals with skin-related conditions, marking curcumin as a versatile and promising agent in the field.
... Twenty rats received an intramuscular injection of 250 mg/kg of body weight of testosterone (Durateston, Schering-Plough, USA) once a week for 4 weeks (De Paiva Gonçalves et al., 2022;Steffens et al., 2018;Steffens et al., 2012). This treatment was performed until the end of the experimental period and aimed to increase the supraphysiological level of testosterone in the animals (Fig. 1). ...
... Part of the histological sections was immunolabeled against factor VIII (Anti-Factor VIII antibody ab61910, Abcam, United Kingdom) by immunohistochemistry assay (IHC) (De Paiva Gonçalves et al., 2022;Wang et al., 2008). The protocol was previously optimized, and the target proteins were detected using the DAB-streptavidin system (LSAB-2, Dako Cytomation, Agilent, USA). ...
Objectives:
This study aimed to evaluate the inflammatory profile as well as the resolution of inflammation in a ligature-induced periodontal inflammation in rats with depletion and/or supraphysiological testosterone replacement.
Design:
Sixty male rats (Holtzman) were used in the present study. Study groups were created as following: (1) Sham (no testicle removal); (2) Orchiectomy (OCX), 3) OCX + Testosterone (OCX + T); (4) Sham + Ligature (SH + L); (5) OCX+L; and 6) OCX + T + L. The surgeries were performed on day 1, and testosterone was administered weekly since day 1. On day 15, a cotton ligature was placed around the lower first molars and maintained for 15 days. Morphological changes in periodontal tissues were determined by histopathological analysis. Immunohistochemistry (factor VIII) and immunoenzymatic assay were performed to evaluate angiogenesis process and (pro- and anti-) inflammatory markers, respectively.
Results:
Ligature promoted a marked inflammatory gingival infiltrate and bone loss (P < 0.05). Supraphysiological testosterone treatment increased the percentage of blood vessels, extracellular matrix and fibroblasts in the presence and absence of periodontal inflammation (P < 0.05). A high dose of testosterone increased factor VIII+ blood vessels and IL-10 expression in inflamed gingival tissue, while PGE2, LXA4 and MPO were reduced as a result of supraphysiological testosterone administration (P < 0.05).
Conclusions:
These results, in our experimental model, suggest that supraphysiological testosterone treatment stimulated gingival tissue repair during ligature-induced periodontitis, and it seems to be related to an anti-inflammatory and pro-resolutive mechanism resulting by the modulatory effect on PGE2 and IL-10 related to an enhanced angiogenesis.
