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Septoria protearum (CBS 135979): a. Culture on MEA, b. Culture on OA, c. Conidiomata on stinging nettle stem, d-f, Conidiogenous cells, g-i. Conidia (Bars = 10 µm).
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The genus Septoria includes important plant pathogens with worldwide distribution, commonly associated with leaf spots and stem cankers of a broad range of plant hosts. In this study eight isolates of Septoria were recovered from leaves with leaf spot on four herbaceous and woody plants from Ardabil, Guilan, East and West Azarbaijan provinces in no...
Citations
... The shape of fungi, including the genus Septoria, is affected by the environment, but DNA sequences are not [5]. Many phylogenetic researches based on DNA sequences have been conducted to get an alternative resolution for the fungal taxonomy [5][6][7][8][9][10][11][12][13]. It has been shown that multigene sequence analyses provided better correlation the DNA-based phylogenies with the morphological traits allowing accuracy on species identification. ...
Leaf spot on lemon balm is frequently observed in Korea, causing considerable damage to crops. In 2014 and 2015, the occurrence of leaf spot was observed in several production greenhouses at Suwon, Gongju, and Namwon in Korea. Symptoms on lower leaves initially developed as small, distinct, discolored lesions, which enlarged progressively turning into dark brown, angular spots surrounded by purplish-brown margins. Based on the morphological characteristics and sequence analysis of actin (ACT), translation elongation factor 1-alpha (EF-1α), internal transcribed spacer (ITS), 28S nrDNA (LSU), and RNA polymerase II second largest subunit (RPB2), the fungus associated with the lemon balm leaf spot was determined as Septoria melissae. To the best of our knowledge, this is the first report of lemon balm leaf spot caused by S. melissae in Asia as well as in Korea.
... To date, there have been several studies focused on Septoria-like fungi in Iran, and more than 100 Septorialike taxa, including the species related to the genera Caryophylloseptoria, Parastagonospora, Parastagonosp orella, Phloeospora, Septoria, Sphaerulina, Stagonospora, Stromatoseptoria, and Zymoseptoria, have been identified (Stukenbrock et al. 2012;Bakhshi and Arzanlou 2017a;Crous et al. 2018;Ghaderi et al. 2018;Bakhshi et al. 2019). However, all previous studies have relied exclusively on morphological data, and very few records are supported by cultures and DNA sequence data, rendering these identifications doubtful Verkley et al. 2013). ...
... The current study aimed to identify the Septoria-like strains obtained from the northern and northwestern zones of Iran based on a combination of DNA phylogeny, host range, ecology, and morphological characteristics. Members of Septoria and Septoria-like genera are devastating plant pathogens on a wide range of economically important agricultural crops worldwide, including Iran (Quaedvlieg et al. 2011Verkley et al. 2013;Bakhshi and Arzanlou 2017a;Bakhshi et al. 2019). To date, approximately 109 Septoria-like species are known to occur on different plant species in Iran, including 80 Septoria species, eight Stagonospora, seven Sphaerulina, seven Zymoseptoria, two Parastagonospora, two Phloeospora, one Caryophyllose ptoria, one Parastagonosporella, and one Stromatoseptoria species (Stukenbrock et al. 2012;Bakhshi and Arzanlou 2017a;Crous et al. 2018;Ghaderi et al. 2018;Azimi and Abbasi 2019;Bakhshi et al. 2019). ...
... Members of Septoria and Septoria-like genera are devastating plant pathogens on a wide range of economically important agricultural crops worldwide, including Iran (Quaedvlieg et al. 2011Verkley et al. 2013;Bakhshi and Arzanlou 2017a;Bakhshi et al. 2019). To date, approximately 109 Septoria-like species are known to occur on different plant species in Iran, including 80 Septoria species, eight Stagonospora, seven Sphaerulina, seven Zymoseptoria, two Parastagonospora, two Phloeospora, one Caryophyllose ptoria, one Parastagonosporella, and one Stromatoseptoria species (Stukenbrock et al. 2012;Bakhshi and Arzanlou 2017a;Crous et al. 2018;Ghaderi et al. 2018;Azimi and Abbasi 2019;Bakhshi et al. 2019). Of the 80 Septoria species known from Iran, the identities of only four species, namely, S. convolvuli, S. polygonorum, S. protearum, and S. urticae, have been confirmed based on DNA sequence data (Bakhshi and Arzanlou 2017a). ...
Species of Septoria are commonly associated with leaf spot diseases of a broad range of plant hosts worldwide. During our investigation of fungi associated with leaf spot diseases in northern and northwestern Iran, several Septoria isolates were recovered from symptomatic leaves on different herbaceous and woody plants in the Asteraceae, Betulaceae, and Salicaceae families. These isolates were studied by applying a polyphasic approach including morphological and cultural data and a multigene phylogeny using a combined data set of partial sequences of the 28S nuc rRNA gene (large subunit [28S]), internal transcribed spacer regions and intervening 5.8S nuc rRNA gene (ITS) of the nuc rDNA operon, actin (actA), translation elongation factor 1-α (tef1), calmodulin (cmdA), β-tubulin (tub2), and DNA-directed RNA polymerase II second largest subunit (rpb2). Four novel species are proposed, namely, Septoria eclipticola on Eclipta prostrata, Septoria firouraghina on Cirsium arvense, Septoria guilanensis on Populus deltoides, and Septoria taleshana on Alnus subcordata. All species are illustrated, and their morphology and phylogenetic relationships with other Septoria species are discussed.
Poplar leaf spot disease, caused by Sphaerulina frondicola (syn: Septoria populi), is one of the important and serious diseases of populus trees, which causes significant loss on this host. Considering the importance of this disease, in the present study, the genetic diversity of 138 S. frondicola isolates obtained from populus trees in different regions of East and West Azarbaijan Provinces was analysed using RAPD and ISSR molecular markers. The results indicated
considerable genetic variation amongst the studied populations. The mean genetic diversity for all of the populations using data from all primers was 0.199 at the probability level of 0.001. The lowest genetic differentiation was observed between Hurand and Kaleyber populations (0.352) with the highest gene flow (54.956) and the highest genetic differentiation was observed between Khoy and Bostanabad populations (0.516) with the lowest gene flow (0.468)
which is consistent with geographical distance. Based on Nei's genetic distance, the Bostanabad population showed the highest genetic distance from the other six populations. Haplotype analysis clustered the isolates from a single tree or even single spot in different groups, suggesting the existence of high genetic diversity in a small scale. Overall, the existence of different haplotypes and distribution of genetic diversity in a small scale observed in this study, suggests
occurrence of regular sexual cycle in S. frondicola populations and highlights the role of airborne ascospores as primary inoculum source in disease cycle.
Gaura parviflora Douglas, a flowering plant of the family Onagraceae, is regarded as an invasive plant in China. Based on the morphological features, we identified that Septoria gaurina caused leaf spots in G. parviflora However, the phylogenetic position of S. gaurina remains unknown. Multi‐locus phylogenetic analysis using the internal transcribed spacer (ITS), β‐tubulin, actin (Act), calmodulin (Cal), translation elongation factor 1‐α (EF), and 28S nrDNA (LSU) nucleotide sequences indicated that the isolates belonged to Septoria genus. Pathogenicity tests demonstrated that the isolates produced symptoms on the leaves of G. parviflora. Small, water‐soaked, circular lesions were formed on the leaves inoculated with conidial droplets. Bigger water‐soaked lesion were formed on the leaves inoculated by conidial spraying. To our knowledge, this is the first report of S. cf. gaurina as the causative agent of leaf blotch disease of G. parviflora in China.
