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Cell tensile technique is an important and widely used tool in cell mechanical research. However, the strain control condition in traditional tensile experiments is not satisfied and would result in big errors. These strain errors will seriously impact the experimental accuracy and decrease the reliability and comparability of experimental results....
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... The stretching device is as Zhou et al. [30] depicted. The substrate was placed between two stainless steel clamps and imaged using an industrial camera (JHSM1400f, Shenzhen Jinghang Technology Co. Ltd.). ...
Cell mechanical stretching in vitro is a fundamental technique commonly used in cardiovascular mechanobiology research. Accordingly, it is crucial to measure the accurate strain field of cell substrate under different strains. Digital image correlation (DIC) is a widely used measurement technique, which is able to obtain the accurate displacement and strain distribution. However, the traditional DIC algorithm used in digital image correlation engine (DICe) cannot obtain accurate result when utilized in large strain measurement. In this paper, an improved method aiming to acquire accurate strain distribution of substrate in large deformation was proposed, to evaluate the effect and accuracy, based on numerical experiments. The results showed that this method was effective and highly accurate. Then, we carried out uniaxial substrate stretching experiments and applied our method to measure strain distribution of the substrate. The proposed method could obtain accurate strain distribution of substrate film during large stretching, which would allow researchers to adequately describe the response of cells to different strains of substrate.
Multidimensional force (load) sensors (transducers) are widely used in modern robotics (including tactile feedback for artificial arm, artificial leg, prosthesis), medical cell tensile experiments, cell mechanical research (Zhou et al. in J Healthc Eng 2017:1587670, 2017 [1]; He et al. in J Healthc Eng 2018:8504273, 2018 [2]), engineering systems to measure three-dimensional forces (feedback strains). For research of multidimensional strain force feedback due to fluid flow pressure developed new physical model. During validation via application of this novel method to the SWMTF (South Western Mooring Tests Facility) 10 times precision increase was obtained for the multidimensional force readings. The method is effective for wide range of measuring equipment based on vector sensors including electronic (strain gauge), piezoelectric, fiber optic load sensors, hydraulic, mechanical (in proving rings) load cells. In particular, the developed method is very effective for accelerated (fast) data processing of loading measurements to achieve real-time sensor-guided robot motions (including those for industrial robot arm, real-time actuators).KeywordsMultidimensional mechanical response to fluid flowsMulti-axis load sensorsMultiaxial fatigueForceSensorsStrain condition monitoringTransducersTri-axis load cell sensorsVector load sensorsCalibrationPrecisionReliabilitySmart sensorsEnhance precisionOvercoming sensor degradation
