Figure
Seizure severity and Seizure latency in PTZ and EPO+PTZ groups
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Objective: The effects of erythropoietin (EPO) which has been frequently studied as an anti-epileptic agent, on peripheral tissues have not been investigated. This study investigated the effects on malondialdehyde (MDA), advanced protein oxidation products (AOPP), superoxide dismutase (SOD), prolidase and sialic acid (SA) levels in the heart, kidne...
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Context 1
... of PTZ-induced seizures A single dose of 60 mg/kg PTZ caused generalized tonic clonic epileptic seizures of 4-5 severity according to the Racine's scale. EPO pretreatment significantly reduced the severity of seizures and increased seizures latency (Table 1). As seizures were not observed in the control group, it was not included in the table. ...Similar publications
Aim: The current study investigated the effects of artemisinin on the heart and lung tissue against pentylenetetrazol-induced seizures in mice. For this purpose, malondialdehyde (MDA), advanced oxidation protein products (AOPP), Catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels were evaluated in both tissue homogenates.M...
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... Tissue homogenates were taken into pre-labeled tubes and centrifuged at 14000 g for 20 minutes at +4°C. The resulting supernatants were placed in Eppendorf tubes and stored in a deep freezer at -80°C until working (25,26). ...
... This may bring about oxidative stress and cellular damage in tissues. [22,23] In this study, epileptic seizures were induced by PTZ. We examined the effects of ARS pretreatment on oxidative stress parameters and antioxidant enzymes that may develop due to seizures in heart and lung tissue. ...
... Findings, PTZ has been reported to increase AOPP levels in peripheral organs. [23,33] PTZ increased the level of AOPP in heart tissue, but did not make a significant difference in the lung. In heart tissue, the VPA and ARS-60 groups reduced AOPP relative to both the C and PTZ groups. ...
... [41][42][43] In previous studies, it has been reported that PTZ application triggers seizure formation by increasing oxidative stress and may cause damage to peripheral tissues. [23,44] To our knowledge, no studies were found investigating the effect of ARS on GSH, GSH-Px enzymes. However, it was reported that Artemisia annua extract, from which ARS was isolated, increased the decreased GSH and GSH-Px levels in rats treated with DMBA (7,12-dimethylbenz[a]anthracene) and exhibited antioxidant activity. ...
Aim: The current study investigated the effects of artemisinin on the heart and lung tissue against pentylenetetrazol-induced seizures in mice. For this purpose, malondialdehyde (MDA), advanced oxidation protein products (AOPP), Catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels were evaluated in both tissue homogenates.Material and Method: Swiss albino male mice (n=42) were used in the experiment. Animals were divided into six groups; Control (C), pentylenetetrazol (PTZ), valproate 100 mg/kg (VPA), artemisinin 30 mg/kg (ARS)+PTZ, ARS 60 mg/kg+PTZ, ARS 120 mg/kg+PTZ. On the 26th day of the experiment, the mice were sacrificed and the samples were kept at -80 0C for biochemical analysis.Results: There were significant differences in the five biochemical parameters analyzed in heart and lung tissues. Heart and lung MDA levels of the PTZ group were found to be significantly higher than the C and ARS-60 groups (p<0.05). Heart and lung MDA levels of the PTZ group were found to be significantly higher than the C and ARS-60 groups. Likewise, heart AOPP levels decreased significantly in the VPA and ARS-60 groups compared to the PTZ group (p<0.05). There was no significant difference between the groups in terms of lung AOPP levels (p>0.05). Heart CAT and GSH levels were decreased in the PTZ group compared to the other groups. However, in terms of Lung CAT levels, the PTZ group had the highest value compared to the other groups, while it had the lowest value in terms of GSH level. The GSH-Px level did not differ significantly between the groups in heart tissue (p>0.05). The lung GSH-Px level was significantly increased in the ARS-30 group when compared to the PTZ group (p<0.05).Conclusion: Consequently ARS treatment can inhibit PTZ-induced oxidative stress in peripheral tissues. In addition, ARS may provide improvements in decreased antioxidant enzymes. ARS may contribute to the antioxidant defense system.
... They also suggested that total antioxidant capacity decreased (Tousson et al. 2019). In their study conducted by different research groups, they reported that PTZ administration increased lipid peroxidation in the kidney, but there was no change in endogenous antioxidant levels (Kapucu et al. 2021;Uma Devi et al. 2006). As a matter of fact, in our study, it was determined that TOS levels increased and TAS levels decreased in the kidneys of mice treated with PTZ. ...
... In recent studies, it has been hypothesized that PTZ administration causes OS by activating glutamate receptors. It has been reported that these receptors may be the source of OS occurring in peripheral organs due to seizures (Kapucu et al. 2021;Lüttjohann et al. 2009). ART pretreatment did not significantly improve total oxidant/antioxidant levels in the kidneys of PTZ-treated mice. ...
This study was designed to investigate the changes in total oxidant (TOS)/antioxidant (TAS) and oxidative stress index (OSI) levels in liver and kidney tissues of mice pre-treatment of artemisinin against oxidative stress that may occur in mice administered pentylenetetrazole (PTZ). Swiss albino mice (Male) (n=42) were used in the study. The mice were divided into six groups and each group had seven animals (n=7): (1) Control (C) /saline Group, (2) PTZ (35 mg/kg) Group, (3) Valproate (VPA) (100 mg/kg)+PTZ Group, (4) Artemisinin (ART) (30 mg/kg)+PTZ Group, (5) ART (60 mg/kg)+PTZ Group, (6) ART (120 mg/kg)+PTZ Grubu. Mice received injections intraperitoneally (i.p.). After the treatments, the animals were observed for seizures for 30 minutes. On the last day (day 26) of the experiment, the PTZ loading dose (75 mg/kg) was administered to the mice and then the animals were sacrificed. TAS, TOS and OSI levels were measured in liver and kidney tissue. PTZ increased TOS and decreased TAS in liver and kidney tissue. ART significantly increased TAS and decreased TOS in liver tissue at increasing doses (p
