Figure 2 - available via license: Creative Commons Attribution-NonCommercial 4.0 International
Content may be subject to copyright.
Sections passing through the deep cerebellar nuclei of the mouse brain showing AChE activity. D: nucleus dentatus; F: nucleus fastigii; I: nucleus interpositus; a: control group; b: lindane treated group; c: antioxidants treated group; d: antioxidants + lindane treated group. Magnification; x25.
Source publication
Objective: The present investigation ascertains a protective potential of a combination of antioxidants against lindane-induced toxicity in cerebellum of mice. Methods: For the study, animals are divided into four groups. First group is control and it is given only vehicle. Second group is treated with lindane and analysed if there are any lesions...
Similar publications
Background: Cypermethrin (Cyp), a synthetic pyrethroid developed for agricultural and public health applications, has become harmful to the environment and mammalian life owing to indiscriminate use.
Aim: This study aimed to investigate the protective effect of the methanolic extract of Jatropha gossypifolia leaves against Cyp-induced genotoxicity...
Objective: Antioxidant probably can prevent the progression and complications of Type 2 diabetes mellitus (T2DM). Due to effectiveness of alpha lipoic acid (ALA) as an antioxidant, this study was done in T2DM patients to evaluate the effect of ALA on their diabetic status, lipid profile, and oxidative stress (OS) status.Methods: A total of 35 patie...
Radical scavenging efficacy of L-cysteine (L-Cys), glutathione (GSH) and thioctic acid (TA) in the presence of silver nanoparticles (AgNPs) were determined by 1,1-diphenyl 2-picryl hydrazil (DPPH), nitric oxide (NO) and hydroxyl (OH) radicals as spectrophotometric assay. The hydrogen peroxide (H 2O
2) scavenging efficacy has been determined by titr...
The present study was designed to investigate the roles of P2X3 receptors in dorsal root ganglion (DRG) neurons in colonic hypersensitivity and the effects of alpha-lipoic acid (ALA) on P2X3 receptor activity and colonic hypersensitivity of diabetic rats. Streptozotocin (STZ) was used to induce diabetic model. Abdominal withdrawal reflex (AWR) resp...
Citations
... After being accumulated in the brain, lindane alters the phospholipids and fatty acid composition as well as fluidity of the membrane, consequently leading to dysfunction of membrane-bound adenosine triphosphatase (ATPase) and acetylcholinesterase (AChE) activity [3][4][5]. In addition to membrane perturbation, lindane enhances oxidative stress by interacting with the cell membrane, triggering the generation of reactive oxygen species (ROS) and altering the level of antioxidant molecules and enzymes. ...
Background:
Current study evaluated the protective potential of quercetin against lindane induced toxicity in mice brain. For investigation, mice were allocated into four groups; First group was control. Second group was administered with oral dose of lindane (25 mg/kg bw) for 4 consecutive days. Third group was exposed to quercetin (40 mg/kg bw) and in fourth group, quercetin was administered 1 hour prior to the exposure of lindane.
Objective:
Two major objectives were decided for study. First was to create lesions in the brain by lindane and; second was to evaluate the neuroprotective potential of quercetin.
Methods:
To study oxidative responses, level of thiobarbituric acid reactive substances (TBARS), protein carbonyl content (PCC), reduced glutathione (GSH), superoxide dismutase (SOD), Catalase (CAT), and glutathione peroxidase (GPx) were measured in brain homogenates. Three key step regulating enzymes of tricarboxylic acid (TCA) cycle viz citrate synthase (CS), pyruvate dehydrogenase (PDH) and fumarase were also assayed.
Results:
Lindane treatment significantly enhanced the levels of TBARS (P<0.001),PCC (P<0.001), GPx (P<0.001), SOD (P<0.05), PDH (P<0.05) and fumarase (P<0.001) in brains of mice compared to control. Meanwhile, it alleviated GSH, CAT and CS (P<0.05) activity.
Conclusion:
Pretreatment with quercetin in lindane treated group not only restored, previously altered biochemical parameters after lindane treatment and also significantly improved them too which suggests that quercetin is not only invulnerable rather neuroprotective against lindane intoxication.
... Similar results were obtained by Kumar et al. (2009) and Sharma et al. (2014). AChE has two sites which bind to the cationic and esteric domains of acetylcholine, cleaving the molecule between these sites (Bist et al., 2013). α-CYP may show AChE inhibitory activity by interacting with anionic substrate binding site (Sharma et al., 2014). ...
Alfa cypermethrin is a pyrethroids extensively used as ectoparasiticide in domestic animals, insecticidal spray on cotton, vegetables and other crops and to kill cockroaches, fleas and termites in house and other buildings. Previous studies have shown the adverse effect of α- cypermethrin on brain. This study was planned to evaluate the possible role of α-lipoic acid in α -cypermethrin induced toxicity in brain of male albino rats. Rats were divided into four groups. The control, α-cypermethrin, α-lipoic acid and α -cypermethrin plus α-lipoic acid treated groups. The duration of the experiment was four weeks. Our results showed that the administration of α-cypermethrin caused a significant decreased in γ- aminobutyric acid level, acetylcholinesterase, catalase, superoxide dismutase activities and increase in lipid peroxidation in cerebellum. Furthermore, the co-administration of α-lipoic acid mitigates the toxicity of α-cypermethrin by partially normalizing the biochemical parameters. The biochemical observations were supported by histopathological examinations. The findings of this investigation suggest that α-lipoic acid may play a protective role against α-cypermethrin induced toxicity in cerebellum of treated rats.
... There is shown an increase in serum MDA levels of dichlorvos exposed mice when compared with control group (Fig. 1). MDA, a reactive species which is one of the LPO end products and used as redox marker, is measured for elevated oxidative stress [44]. Earlier studies also had shown that the high amount of dichlorvos stimulate LPO through increasing plasma MDA levels [45]. ...
... GSH is a non-enzymatic antioxidant which plays a key role in the detoxification of xenobiotic compounds, ROS and free radicals. Increasing oxidative stress is usually accompanied by a decline in GSH level [44]. Intoxication with dichlorvos is reported to induce an oxidative stress leading to generation of ROS and free radicals that convert GSH to oxidized form GSSG [40,56]. ...
Reactive oxygen species (ROS) may be involved in the pathogenesis of serum induced by dichlorvos. Therefore, the rationale of present research was to evaluate the ameliorative efficacy of bacoside A and bromelain on oxidative stress biomarkers in serum of dichlorvos intoxicated mice. Also the level of serum antioxidants viz. catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and reduced glutathione (GSH) were measured. For experiments, mice were allocated into six groups. First group received saline as a vehicle; second group was administered with dichlorvos (40 mg/kg b.w.); third group was administered with bromelain (70 mg/kg b.w.), fourth group received dose of bacoside A (5 mg/kg b.w.), fifth group was given concomitant exposure of bacoside A and bromelain both and mice of sixth group were exposed to bacoside A, bromelain and dichlorvos for 21 days consecutively. Oxidative stress biomarkers thiobarbituric acid reactive substances (TBARS) and protein carbonyl content (PCC) and antioxidants (CAT, SOD, GPx and GSH) level of serum was determined to elucidate the protective potential of bacoside A and bromelain against dichlorvos intoxication. Significantly increased TBARS and PCC level in second group suggests that dichlorvos enhances the production of free radicals in serum of mice (p < 0.05). Antioxidants treatment significantly decreased the levels of TBARS and PCC (p < 0.05). Dichlorvos administration causes a significant reduction in the level of CAT, SOD, GPx and GSH (p < 0.05) which was restored significantly by co-administration of bromelain and bacoside A in dichlorvos exposed mice (p < 0.05). The bacoside A and bromelain are attributed with antioxidant properties. Finding of research conclude that concomitant exposure of bacoside A and bromelain was much effective in combating oxidative stress induced by dichlorvos.
