Fig 1 - available from: Cerebellum & Ataxias
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Section of cerebellum showing granular layer interneurons stained with different methods. Upper should be changed into a: Stained section with acidic Madder for 2 h and Bottom should be changed into b: with Madder for 24 h. Magnification 200×
Source publication
Background
The objective of the present study was an investigation of root Rubia Tinctorum (Madder) as a natural dye to identification of granular layer interneurons of the rat cerebellum.
Methods
Seven to ten micrometre sections were collected from the cerebellum and stained only with Madder for 2, 24 and 48 h. Other sections were stained with Ma...
Citations
... On the other hand, several synthetic special stains are also available to stain these structures. [16][17][18][19][20][21] Adisa et al. studied the use of henna leaves extract as a natural cytoplasmic stain in liver tissues and found that hepatocytes stained golden brown color with well-defined cytoplasmic boundary and concluded that henna leaves extract could be a suitable cytoplasmic stain in histopathology. [16] Similarly, in 2015, Ajileye et al. conducted a study to explore the staining potential of Zingiber officinale (ginger) extract on tissue sections and found that nuclei stained deeply green color whereas the cytoplasm and muscle fibers showed yellow color. ...
Background:
The pharmacological actions of Curcuma aromatica (wild turmeric) such as anti-inflammatory, antitumor, antifungal, antimicrobial and wound healing have been recognized since ages. However, its role as a natural histological stain has not been explored till date.
Aim:
To evaluate the efficacy of natural substance-Kumkum prepared from the extract of C. aromatica and slaked lime in staining the biopsied oral tissues.
Materials and methods:
A cohort study that used 60 formalin fixed paraffin embedded soft and hard tissue specimens from institutional archives were subjected to sectioning and stained using Kumkum and hematoxylin and eosin (H and E). The slides were evaluated for their staining efficacy and results were statistically analyzed using Wilcoxon signed-rank test and Independent 't' test.
Results:
The mean of the overall parameters assessed for staining efficacy did not show statistically significant difference between the study groups in normal and pathological specimens for tooth (P = 0.410 and 0.484), bone (P = 0.133 and 0.157) and soft tissues (P = 0.186 and 0.113), respectively. This suggests that Kumkum staining efficacy is equivalent to that of routine H and E for oral tissues. Structures such as dentinoenamel junction, dentinal tubules, incremental lines of cementum, reversal and resting lines, osteocytic canaliculi, mature and immature bone could be appreciated better in Kumkum stained slides, thereby rendering a special staining property to Kumkum stain.
Conclusion:
To our knowledge, this study is the first of its kind to have used Kumkum stain obtained from C. aromatica for the differentiation of the components of tooth, bone and soft tissue structures in histostaining of oral tissues. The naturally prepared Kumkum stain possesses dual staining property both in routine and differential staining. This facilitates diagnosis of fibro-osseous lesions, bony, collagen and muscular pathologies. The natural stain also finds application in forensic odontology for age estimation.
La lévodopa est actuellement la thérapie de référence pour les patients atteints de la maladie de Parkinson et permet le rétablissement artificiel des niveaux de dopamine. Cependant, ce traitement s'accompagne de mouvements involontaires anormaux invalidants, ou dyskinésies. Au cours de ma thèse, j'ai étudié l'implication des cellules de Purkinje de la région cérébelleuses crus II, via les voies cérébello-thalamo-cortico-striatale et cérébello-thalamo-striatale, dans l'émergence et le traitement des mouvements involontaires anormaux oro-linguaux induits par la lévodopa dans un modèle murin de la maladie de Parkinson. Mes résultats suggèrent que la stimulation chronique et spécifique des cellules de Purkinje par une technique d'optogénétique semble à même de corriger et de prévenir le phénotype dyskinétique et ce en association avec une modulation de l'activité du noyau thalamique intralaminaire parafasciculaire, du cortex moteur primaire oral et du striatum dorsal. Les voies cérébello-thalamo-cortico-striatale et cérébello-thalamo-striatale semblent ainsi impliquées dans le traitement correctif et préventif des dyskinésies induites par la lévodopa dans la maladie de Parkinson.
