Screenshot of Adobe ® Audition TM CS6 software. Spectrograms and frequency analysis of an extracted sound. 

Screenshot of Adobe ® Audition TM CS6 software. Spectrograms and frequency analysis of an extracted sound. 

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Article
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In this study, we describe the monitoring of young broiler chicken vocalisation, with sound recorded and assessed at regular intervals throughout the life of the birds from day 1 to day 38, with a focus on the first week of life. We assess whether there are recognisable, and even predictable, vocalisation patterns based on frequency and sound spect...

Contexts in source publication

Context 1
... Adobe ® Audition TM CS6, a number of discrete sound 'groups' were identified and analysed using time (x-axis) and frequency (y-axis) for further statistical comparisons. A fast fourier transform (FFT) was used to perform the frequency analyses using a Hamming window with a FFT dimension of 256 (Figure 1). Figure 1 shows the spectrogram of a labelled vocalisation. ...
Context 2
... fast fourier transform (FFT) was used to perform the frequency analyses using a Hamming window with a FFT dimension of 256 (Figure 1). Figure 1 shows the spectrogram of a labelled vocalisation. The time is reported on the x-axis and the frequency on the y-axis. ...
Context 3
... results in capturing sounds within the broiler house where the chicks tended to cluster ‘ in groups ’ classi fi ed as house sounds (HS). At the same time as the sound recordings were being made, video recordings were acquired by placing a digital video camera on a low-level tripod focussed on the area where the birds were most active and in the locality of the microphones. After each period of continuous recording, three chicks, chosen at random, were moved into an enclosed ‘ shielded recording area ’ (30 cm high box with an area of 0.8 m 2 ), in order to collect individual bird sounds of ‘ isolated ’ chicks by shielding the microphone from background environmental noise. The chicks were individually placed into the separation box at times 0 (chick 1), 1 min later (chick 2) and 2 min later (chick 3); 5 min of recording box sounds (BS) were initiated when the fi rst chick was placed in the box. Simultaneously with the BS audio recordings video recordings were made, positioning the video of the chicks inside the box, and also of chicks in the area just outside the box. After 5 min of recording, the barrier was removed and the chicks were returned to the fl ock. The fi nal sound ‘ library ’ consisted of 27 h 24 min of sound recordings for trial 1 and 27 h 56 min of sound recordings for trial 2. It was decided to analyse and manually label the vocalisations recorded with MIC 1 (bird focussed) due to the higher quality of the sounds compared with the ones recorded with MIC 2 (background focussed). In this study, the sounds (HS and BS) recorded during Day 1 and Day 5 in both trials have been analysed. Day 1 and Day 5 were chosen in order to provide a time interval appropriate to examine the difference of the sounds emitted within the fi rst week of the birds life. Sound recordings were manually analysed of fl ine and labelled using sound analysis software: Adobe ® Audition TM CS6. Sound labelling involved the extraction and classi fi cation of both individual ‘ isolated ’ animal sounds (BS) and general sounds coming from the whole fl ock (HS). Analysis examined amplitude and frequency of the sound signals in audio fi les, each fi le being manually labelled using a procedure based on acoustic analysis combined with visual spectral analysis, used to extract recognisably distinct and characteristic sound patterns from the entire recording fi le. Every hour long digital recording was cut in shorter labelled fi les of 10 min duration in order to facilitate the sound analysis and the labelling procedure. The sound analysis was divided into two different phases. First, the fi le was examined (lis- tened to with high frequency response headphones) in its entirety in order to recognise the regions of the recording with the clearest sounds. During the ‘ listen through ’ , the regions of the recording with the clearest sounds were then digitally marked ( fl agged) in order to classify different types of sound. The methodology for the labelling procedure and the sound analysis is that described by (Marx et al., 2001). The labelling procedure was carried out of fl ine, identifying those sounds that the operator classi fi ed as signi fi cant vocalisation sounds through the combination of auditive analysis (listening with headphones) and the visual observation of the spectrogram of the sounds corresponding to each sound ‘ group ’ (Ferrari et al., 2008). Through Adobe ® Audition TM CS6, a number of discrete sound ‘ groups ’ were identi fi ed and analysed using time (x-axis) and frequency (y-axis) for further statistical comparisons. A fast fourier transform (FFT) was used to perform the frequency analyses using a Hamming window with a FFT dimension of 256 (Figure 1). Figure 1 shows the spectrogram of a labelled vocalisation. The time is reported on the x-axis and the frequency on the y-axis. Bright areas indicate sounds with high energy. The small box on the right shows the frequency analysis of the marked sound on the left. The mean duration, the mean interval and the number of repetitions of each kind of vocalisation were collected. For both HS and BS, the peak frequency (PF = representing the frequency of maximum power) was manually extracted. The frequency range was band pass fi ltered between 1000 and 13 000 Hz. The lower frequency limit was set at 1000 Hz to remove the low frequency background noise and the upper limit was set at 13 000 Hz to cut off high frequency noise and also because broilers are sensitive to a frequency range of about 60 to 11 950 (Appleby et al., 1992; Tefera, 2012). Video and sound recordings were synchronised during the labelling procedure in order to link the behaviours to the sounds emitted by the animals. Statistical analysis was performed using statistical software SAS 9.3. The difference in PF of HS and BS recorded during Day 1 and Day 5 were tested using a paired t-test in order to evaluate whether there were statistically signi fi cant changes in vocalisation PF related to different days and situations (isolated/in group). Paired sample t-tests were made to compare the PF of vocalisations emitted by the chicks in six speci fi c situations: a) BS collected in Day 1 and in Day 5 (BS1-BS5). b) HS collected in Day 1 and in Day 5 (HS1-HS5). c) BS and HS collected in Day 1 (BS1-HS1). d) BS and HS collected in Day 5 (BS5-HS5). e) BS collected in Day 1 and HS collected in Day 5 (BS1-HS5). f) BS collected in Day 5 and HS collected in Day 1 (BS5-HS1). Statistical analysis of sounds was performed using the clearest vocalisations (the loudest, the ‘ clearest ’ and with the highest energy) found during the labelling procedure of recordings made on Day 1 and Day 5. The fi nal dataset consisted of 60 BS (isolated chicks) sound fi les, and 136 sound fi les from HS. The correlations between BS and HS were evaluated to identify whether chicks emitted speci fi c sounds on a speci fi c day (1 or 5) or speci fi c sounds during a stress situation (isolated or in group). A further comparison among the different BS sounds was fi rst performed analysing the spectrogram of each sounds (visual analysis) and then using the PDIFF (differences between least squares means) option in the GLM procedure of SAS to verify their similarity/ dissimilarity (SAS User ’ s Guide, ...
Context 4
... results in capturing sounds within the broiler house where the chicks tended to cluster ‘ in groups ’ classi fi ed as house sounds (HS). At the same time as the sound recordings were being made, video recordings were acquired by placing a digital video camera on a low-level tripod focussed on the area where the birds were most active and in the locality of the microphones. After each period of continuous recording, three chicks, chosen at random, were moved into an enclosed ‘ shielded recording area ’ (30 cm high box with an area of 0.8 m 2 ), in order to collect individual bird sounds of ‘ isolated ’ chicks by shielding the microphone from background environmental noise. The chicks were individually placed into the separation box at times 0 (chick 1), 1 min later (chick 2) and 2 min later (chick 3); 5 min of recording box sounds (BS) were initiated when the fi rst chick was placed in the box. Simultaneously with the BS audio recordings video recordings were made, positioning the video of the chicks inside the box, and also of chicks in the area just outside the box. After 5 min of recording, the barrier was removed and the chicks were returned to the fl ock. The fi nal sound ‘ library ’ consisted of 27 h 24 min of sound recordings for trial 1 and 27 h 56 min of sound recordings for trial 2. It was decided to analyse and manually label the vocalisations recorded with MIC 1 (bird focussed) due to the higher quality of the sounds compared with the ones recorded with MIC 2 (background focussed). In this study, the sounds (HS and BS) recorded during Day 1 and Day 5 in both trials have been analysed. Day 1 and Day 5 were chosen in order to provide a time interval appropriate to examine the difference of the sounds emitted within the fi rst week of the birds life. Sound recordings were manually analysed of fl ine and labelled using sound analysis software: Adobe ® Audition TM CS6. Sound labelling involved the extraction and classi fi cation of both individual ‘ isolated ’ animal sounds (BS) and general sounds coming from the whole fl ock (HS). Analysis examined amplitude and frequency of the sound signals in audio fi les, each fi le being manually labelled using a procedure based on acoustic analysis combined with visual spectral analysis, used to extract recognisably distinct and characteristic sound patterns from the entire recording fi le. Every hour long digital recording was cut in shorter labelled fi les of 10 min duration in order to facilitate the sound analysis and the labelling procedure. The sound analysis was divided into two different phases. First, the fi le was examined (lis- tened to with high frequency response headphones) in its entirety in order to recognise the regions of the recording with the clearest sounds. During the ‘ listen through ’ , the regions of the recording with the clearest sounds were then digitally marked ( fl agged) in order to classify different types of sound. The methodology for the labelling procedure and the sound analysis is that described by (Marx et al., 2001). The labelling procedure was carried out of fl ine, identifying those sounds that the operator classi fi ed as signi fi cant vocalisation sounds through the combination of auditive analysis (listening with headphones) and the visual observation of the spectrogram of the sounds corresponding to each sound ‘ group ’ (Ferrari et al., 2008). Through Adobe ® Audition TM CS6, a number of discrete sound ‘ groups ’ were identi fi ed and analysed using time (x-axis) and frequency (y-axis) for further statistical comparisons. A fast fourier transform (FFT) was used to perform the frequency analyses using a Hamming window with a FFT dimension of 256 (Figure 1). Figure 1 shows the spectrogram of a labelled vocalisation. The time is reported on the x-axis and the frequency on the y-axis. Bright areas indicate sounds with high energy. The small box on the right shows the frequency analysis of the marked sound on the left. The mean duration, the mean interval and the number of repetitions of each kind of vocalisation were collected. For both HS and BS, the peak frequency (PF = representing the frequency of maximum power) was manually extracted. The frequency range was band pass fi ltered between 1000 and 13 000 Hz. The lower frequency limit was set at 1000 Hz to remove the low frequency background noise and the upper limit was set at 13 000 Hz to cut off high frequency noise and also because broilers are sensitive to a frequency range of about 60 to 11 950 (Appleby et al., 1992; Tefera, 2012). Video and sound recordings were synchronised during the labelling procedure in order to link the behaviours to the sounds emitted by the animals. Statistical analysis was performed using statistical software SAS 9.3. The difference in PF of HS and BS recorded during Day 1 and Day 5 were tested using a paired t-test in order to evaluate whether there were statistically signi fi cant changes in vocalisation PF related to different days and situations (isolated/in group). Paired sample t-tests were made to compare the PF of vocalisations emitted by the chicks in six speci fi c situations: a) BS collected in Day 1 and in Day 5 (BS1-BS5). b) HS collected in Day 1 and in Day 5 (HS1-HS5). c) BS and HS collected in Day 1 (BS1-HS1). d) BS and HS collected in Day 5 (BS5-HS5). e) BS collected in Day 1 and HS collected in Day 5 (BS1-HS5). f) BS collected in Day 5 and HS collected in Day 1 (BS5-HS1). Statistical analysis of sounds was performed using the clearest vocalisations (the loudest, the ‘ clearest ’ and with the highest energy) found during the labelling procedure of recordings made on Day 1 and Day 5. The fi nal dataset consisted of 60 BS (isolated chicks) sound fi les, and 136 sound fi les from HS. The correlations between BS and HS were evaluated to identify whether chicks emitted speci fi c sounds on a speci fi c day (1 or 5) or speci fi c sounds during a stress situation (isolated or in group). A further comparison among the different BS sounds was fi rst performed analysing the spectrogram of each sounds (visual analysis) and then using the PDIFF (differences between least squares means) option in the GLM procedure of SAS to verify their similarity/ dissimilarity (SAS User ’ s Guide, ...

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