Fig 3 - uploaded by Priti Nirav Desai
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Scratch wound assay. A) Images from three independent experiments captured at 24, 48, and 72 h (40 × total magnification). B) Quantitative values of a wound area left after the migration of cells at different time points. Mock cells (black bars), pSUPER.neo-transfected cells (light gray bars), and psNIPERDH1C1-transfected cells (dark gray bars, bars s.e.m)
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Vascular endothelial growth factor C (VEGF-C) is known to stimulate growth of endothelial cells to form tumor-associated lymphatics, which leads to the migration of tumor cells to distant places. However, the function of VEGF-C is not limited to lymph angiogenesis only. VEGF-C is produced by breast cancer cells and binds to the receptors present on...
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Context 1
... scratch and wound assay displayed a migratory nature of the mock and pSUPER.neo-transfected cells as compared to psNIPERDH1C1-transfected cells after 72 h. The mock and pSUPER.neo-transfected cells mi- grated toward the site of wound leading to its closure while the gap remained unsealed in case of VEGF-C-si- lenced cells, as shown in images (Fig. 3A). The quantita- tive values of wound size were determined by ImageJ software. For multiple comparisons, Dunnett's test was applied. According to this, insignificant difference was ob- served between mock cells and pSUPER.neo-transfected cells at any time point. Whereas significant difference was observed between gap closure of ...
Context 2
... determined by ImageJ software. For multiple comparisons, Dunnett's test was applied. According to this, insignificant difference was ob- served between mock cells and pSUPER.neo-transfected cells at any time point. Whereas significant difference was observed between gap closure of pSUPER.neo-transfected cells and psNIPERDH1C1-transfected cells (Fig. ...
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