Scheme: Synthesis of novel chalcone derivatives Identification tests TLC: Merck silica gel 60 F 254 Al-backed plates were used with methanol: ethyl acetate (1:1) solvent system and the plates are stained with phosphomolybdic acid and tested under UV lamp at 254 nm [1]  

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Context 1

... mixture was filtered through vaccum washed with water, and then it was crystallized using a mixture of ethyl acetate and methanol (8:2) to afford compounds from RC-1 to RC-8. Figure 1 shows scheme for synthesis of novel chalcones derivatives. Wilson test: The synthesized compounds were treated with conc. ...

Context 2

... in vitro screening of antibacterial activity was carried out by using Cylinder-plate assay method. For antibacterial activity, the inoculums or microbial suspension is prepared according to the procedure given in the I.P (Indian pharmacoepia-2010).The test organism (one loop full) were seeded into the nutrient agar medium (HIMEDIA) at temperature between 40° to 50° and immediately pour the inoculated medium into the Petri plate (8 Inch) to give a depth of 3 to 4 mm and allowed to solidify and punched with a sterile cork borer (6.0 mm diameter) to make open cavities. Each plate should have cavities with appropriate distances. ...