Fig 1 - uploaded by Prasanna K. Santhekadur
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Schematic representation of miRNA biogenesis and RISC assembly. miRNAs play an important role in the regulation of genes. Primary miRNAs are cleaved in the nucleus to generate precursor miRNAs. Furthermore, precursor miRNAs undergo processing in the cytoplasm to form a miRNA duplex. RISC, via its catalytic AGO2 or SND1, specifically incorporates the guide strand, which is base paired with the target mRNA, leading to gene silencing. AGO2, argonaute2; miRNAs, microRNAs; mRNA, messenger RNA; RISC, RNA-induced silencing complex; SND1, Staphylococcal nuclease and Tudor domain-containing 1.
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Hepatocellular carcinoma (HCC) is one of the most lethal and widespread cancers in the human race. Despite its fatal attributes, there is only a limited understanding of the factors contributing to its pathogenesis at the cellular and molecular levels.
Consequently, unraveling new facets involved in HCC progression is elemental for establishing no...
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... of double-stranded RNA in the eukaryotic cytoplasm, which is then modified into compact regulatory RNAs (20-30 nucleotides in length). 4 Argonaute protein, a prominent protein of RISC, aids in the recruitment of guide miRNA to the 3′-untranslated region of target mRNA and regulates transcript expression through RISC-mediated mRNA cleavage ( Fig. 1). 9,11,12 In the aftermath of target recognition, several patterns of RISC effector processes are exhibited. In one such approach, RISC subjects its complementary mRNA to slicing or hydrolysis by cellular exonucleases, which obstructs protein encoding. 13 In the mammalian system, the mode of gene silencing by RISC is ubiquitously ...
