Figure 7 - uploaded by Pernilla Abrahamsson
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Schematic picture over the heart, placement of the snare over the LAD branch and the MD probe in the myocardium (dotted line) and on the surface of the heart (solid line). The ischemic area is shaded. (Paper III)
Source publication
Background: The microdialysis (MD) technique is widely spread and used both experimentally and in clinical practice. The MD technique allows continuous collection of small molecules such as glucose, lactate, pyruvate and glycerol. Samples are often analysed using the CMA 600 analyser, an enzymatic and colorimetric analyser. Data evaluating the per...
Context in source publication
Context 1
... probes were placed in the myocardial tissue (myocardial probe) supplied by the snared LAD branch. A second MD probe was placed on the epicardial surface (surface probe) in the same area as the myocardial probe ( Figure 7). The probes were secured with one suture placed over the membrane and one suture placed in the fixation point. ...
Citations
... Shorter collection intervals are possible, but were not necessary here. A 15 min interval meant that the sample represents a single averaged value during this period, although brief higher or lower levels for the measured substances could have occurred interstitially [221][222][223] . ...
Objective
To investigate the effect of aortic supra-celiac balloon occlusion (ABO) and superior mesenteric artery (SMA) occlusion on abdominal visceral metabolism in an animal model by using intraperitoneal microdialysis (IPM) and laser Doppler flowmetry.
Methods
Nine pigs were subjected to ABO and seven animals were subjected to SMA occlusion for one hour followed by three hours of reperfusion. Seven animals served as controls. Hemodynamic data, arterial blood samples, urinary output and intestinal mucosal blood flow (IBF) were followed hourly. Intraperitoneal (i.p) glucose, glycerol, lactate and pyruvate concentrations and lactate-pyruvate (l/p) ratio were measured by IPM.
Results
Compared to baseline, ABO reduced IBF by 76% and decreased urinary output. SMA occlusion reduced IBF by 75% without affecting urinary output. ABO increased the i.p l/p ratio from 18 at baseline, peaking at 46 in early reperfusion. SMA occlusion and reperfusion tended to increase the i.p l/p ratio, peaking at 36 in early reperfusion. ABO increased the i.p glycerol concentration from 87 μM at baseline to 579 μM after three hours of reperfusion. SMA occlusion and reperfusion increased the i.p glycerol concentration but to a lesser degree.
Conclusions
Supra-celiac ABO caused severe hemodynamic, renal and systemic metabolic disturbances compared to SMA occlusion, most likely due to the more extensive ischemia-reperfusion injury. The intra-abdominal metabolism, measured by microdialysis, was affected both by ABO and SMA occlusion but the most severe disturbances were caused by ABO. The i.p l/p ratios and the glycerol concentrations increased during ischemia and reperfusion and may serve as markers of these events and indicate anaerobic metabolism and cell damage respectively.
