Figure 2 - uploaded by Andreas Weinhäusel
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Schematic overview of tested samples and Luminex assay conditions. Denatured conditions refer to pre-treatment of the Luminex bead pool with 2% SDS for 10min at 70°C prior to sample incubation while native condition refers to no pre-treatment of the bead-pool.

Schematic overview of tested samples and Luminex assay conditions. Denatured conditions refer to pre-treatment of the Luminex bead pool with 2% SDS for 10min at 70°C prior to sample incubation while native condition refers to no pre-treatment of the bead-pool.

Source publication
Experiment Findings
Full-text available
Antibody reactivities and the respective antigenic proteins and peptides are diagnostically meaningful - protein-arrays are used for discovery of antigenic proteins and confirmation & validation of analysis is best conducted using Luminex bead arrays. Here we show how that the transfer of protein-macroarray derived clones onto Luminex-bead arrays i...