Schematic diagram, micrograph of histology and histochemistry of atretic follicles in Rastrelliger brachysoma ; (A-B) atretic follicle of previtellogenic stage (Ap), A = 100  m, B = 50  m; (D-I) Normal vitellogenic stage (V) = 70  m; (C, K-O) Stage Is = 50  m; (J, P-T) Stage II = 50  m; (U-Z) Stage III = 50  m; (Z1, a-e) Stage IV = 50  m; (Z2, f-j) Stage V = 50  m; Apt = apoptosis, B = basement membrane, Bv = blood vessel, Ca = Cortical alveoli, G = granulosa cell, Hg = hypertrophy of granulosa cell, Lc = lymphocytes, Ly = liquefaction of yolk granules, N = nucleus, P = previtellogenic stage, Pt = pynotic nuclei, T = theca cell, VC = vacuole, Wbc = white blood cells, Y = yolk granules, Zi = inner layer of zonapellucida, Zo = outer layer of zonapellucida, Zp = zonapellucida, * = degeneration of yolk granule, ** = degeneration of cortical alveoli.(MT = Masson’s trichrome, PAS = periodic acid-schiff, AB = aniline blue, Rt = reticulin method). 

Contexts in source publication

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... characterization of atretic follicle in the pre- vitellogenic stage was similarly seen with normal stage, but the ooplasm shown as basophilic cytoplasm with a surrounding thin follicular layer (PAS staining) (Figure 2A and 2B). ...

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... normal vitellogenic stage, histo- logical details of several atretic follicles during vitellogenic stage in this species were detected. It could be completely divided into four steps according to shape, characterizations of nucleus and follicle complex ( Figure 2D to 2I). ...

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... in shape and degeneration with separation between inner and outer layers of the zonapellucida were initially and continuously detected. Additionally, hypertro- phies together with pyknosis of some granulosa and theca cells were detected ( Figure 2C and 2K). Based on histo- chemistry, the cortical alveoli were still positive with MT as reddish and AB as bluish. ...

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... on histo- chemistry, the cortical alveoli were still positive with MT as reddish and AB as bluish. Other characterizations, yolk granules with slight positively stain with PAS reaction, were specially surrounded by basement membrane (black line with RT) ( Figure 2L to 2O). Stage II, the early stage of stage II was irregular in shape. ...

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... hypertrophy of granulosa cells were also continuously proliferated, which each cell had a spherical nucleus with surrounding the eosinophilic cytoplasm. Externally, the thecal cell layer was located around few blood vessels ( Figure 2J and 2P). AB, MT and PAS reactions conformed to previous stage but RT reaction distinctly showed the degeneration due to the fragment of basement membrane ( Figure 2Q to 2T). ...

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... the thecal cell layer was located around few blood vessels ( Figure 2J and 2P). AB, MT and PAS reactions conformed to previous stage but RT reaction distinctly showed the degeneration due to the fragment of basement membrane ( Figure 2Q to 2T). ...

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... granulosa and theca were not separated and rarely seen. Surprisingly, in this stage, the leucocytes were mostly found and continued to be found near oocyte ( Figure 2U and 2V). Histochemically, the cortical alveoli were slightly seen and began to degenerate (AB and MT stains) whereas RT reaction confirmed that no basement membrane was seen during this stage ( Figure 2W to 2Z). ...

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... in this stage, the leucocytes were mostly found and continued to be found near oocyte ( Figure 2U and 2V). Histochemically, the cortical alveoli were slightly seen and began to degenerate (AB and MT stains) whereas RT reaction confirmed that no basement membrane was seen during this stage ( Figure 2W to 2Z). ...

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... the follicular cells became phagocytize degenerating materials because several leucocytes were presented among a few blood vessels ( Figure 2Z1 and 2a). No mucopolysaccharide was observed according to AB and MT, indicating the complete degeneration of cortical alveoli (Figure 2b and 2e). ...

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... follicle (both yolk granules and zona pellucida) itself was completely digested. The large vacuole associated with yellow-brownish pigments within the ooplasm was accumulated ( Figure 2Z2 and 2f). Some follicular cells were also presented. ...

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... follicular cells were also presented. Moreover, this stage was surrounded by fibroblast-like cells, as strongly positive with MT and PAS stained (Figure 2g and 2h). It should be noted that this stage could potentially cause by the apoptosis under RT reaction (Figure 2j). ...

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... this stage was surrounded by fibroblast-like cells, as strongly positive with MT and PAS stained (Figure 2g and 2h). It should be noted that this stage could potentially cause by the apoptosis under RT reaction (Figure 2j). The leucocytes were found around the apoptotic follicles (based on RT stained). ...