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Saccharomyces cerevisiae yeast. (A) Field view (2,000 x, 25.00 kV). (B) Budding scars are observed as bumps on the cell wall (arrow, 37,963 x, 25.00 kV). (C) budding process will leave the characteristic scar on both, the parent cell (PC) and the daughter cell (Y) (birth scar). Bumpy surfaces of mature yeast cells are observed (80,000 x, 25.00 kV). ETD: Everhart-Thornley detector (a secondary electron and back-scattered electron detector).

Saccharomyces cerevisiae yeast. (A) Field view (2,000 x, 25.00 kV). (B) Budding scars are observed as bumps on the cell wall (arrow, 37,963 x, 25.00 kV). (C) budding process will leave the characteristic scar on both, the parent cell (PC) and the daughter cell (Y) (birth scar). Bumpy surfaces of mature yeast cells are observed (80,000 x, 25.00 kV). ETD: Everhart-Thornley detector (a secondary electron and back-scattered electron detector).

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The aim of this study was to develop an easy method for the analysis of the surface ultrastructure in prokaryotic and eukaryotic free cells. The procedure to prepare three types of free cells, the adhesion to poly-L-lysin (PLL)-coated glass substrate, fixation, washing, dehydration in increasing ethanol concentrations, critical point drying, mounti...

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Context 1
... washing steps as well as the concentration adjustment favored the even distribution of the cells on the PLL-SS. In the case of S. cerevisiae sample (Figure 2A), a relatively low magnification (2,000 x) allowed the detection of yeast at initial and final stages of budding. At a 37,963 x magnification, budding scars were clearly distinguished in the parent yeast cells as well as in the bud or daughter cells ( Figure 2B). ...
Context 2
... the case of S. cerevisiae sample (Figure 2A), a relatively low magnification (2,000 x) allowed the detection of yeast at initial and final stages of budding. At a 37,963 x magnification, budding scars were clearly distinguished in the parent yeast cells as well as in the bud or daughter cells ( Figure 2B). Finally, at 80,000 x, small bumps were evident on the surface of parent cells but absent on buds ( Figure 2C), allowing the distinction between aged and newly formed yeast cells. ...
Context 3
... a 37,963 x magnification, budding scars were clearly distinguished in the parent yeast cells as well as in the bud or daughter cells ( Figure 2B). Finally, at 80,000 x, small bumps were evident on the surface of parent cells but absent on buds ( Figure 2C), allowing the distinction between aged and newly formed yeast cells. In actinomycete sample, the continuous of filaments were observed as well as lightly prominent zones, which may be indicative of incipient formation of spores (Figure 3). Figure 3. Vegetative mycelium of actinomycetes (aerobic bacteria). ...