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SEM of antennal sensilla of apterous viviparous female of S. yushanensis: (a) antennal flagellum general view, (b) ANT III with type I trichoid sensilla, (c) ANT IV with type I trichoid sensilla and one small multiporous placoid sensillum (Orange) -secondary rhinarium, (d) ANT V with type I trichoid sensilla, one small multiporous placoid sensillum (Orange) -secondary rhinarium and big multiporous placoid sensillum (yellow) primary rhinarium, (e) ANT VI with type I trichoid sensilla, type II trichoid sensilla (violet), big multiporous placoid sensillum (yellow) -major rhinarium, small multiporous placoid sensilla (green) and sunken coeloconic sensilla (pink) -accessory rhinaria.

SEM of antennal sensilla of apterous viviparous female of S. yushanensis: (a) antennal flagellum general view, (b) ANT III with type I trichoid sensilla, (c) ANT IV with type I trichoid sensilla and one small multiporous placoid sensillum (Orange) -secondary rhinarium, (d) ANT V with type I trichoid sensilla, one small multiporous placoid sensillum (Orange) -secondary rhinarium and big multiporous placoid sensillum (yellow) primary rhinarium, (e) ANT VI with type I trichoid sensilla, type II trichoid sensilla (violet), big multiporous placoid sensillum (yellow) -major rhinarium, small multiporous placoid sensilla (green) and sunken coeloconic sensilla (pink) -accessory rhinaria.

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Here, we present a revision of the poorly studied aphid genus, Sinolachnus from the tribe Tuberolachnini of the subfamily Lachninae (Hemiptera: Aphididae) modified to include eight species. Apterous and alate viviparous females of the type species S. niitakayamensis are redescribed together with alate viviparous females of Sinolachnus elaeagnensis....

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... contrast to type I trichoid sensilla the type II sensilla are characterized by blunt or slightly rounded apices with a granulose surface ( Figure 10(f)). During the examination of the apterous viviparous antennal flagellum besides type I trichoid sensilla, small multiporous placoid sensilla, big multiporous placoid sensilla and sunken coeloconic sensilla have been found (Figure 9(c)-(e)). In the apterae of S. yushanensis, small multiporous placoid sensilla have been found on ANT IV and V as secondary rhinaria and on ANT VI BASE as primary (accessory) rhinaria. ...
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... placoid multiporous sensilla on ANT IV and V are rather small, rounded, slightly protuberant and lie in depressions of the cuticle (Figure 11 )). Most types of sensilla on ANT VI are distributed on the BASE and consist of big multiporous placoid sensillum (major rhinarium) with almost all accessory rhinaria lying on the lateral side of the major rhinarium and one small placoid sensillum moved far from the other ones to the terminal process (Figure 9(e)). The structure and porous surface of the big placoid sensillum (major rhinarium) are the same as on ANT V. ...
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... Figs. 23, 29; Table II) http://urn:lsid:zoobank.org:act:A8A4CCB2-3434-4ADF-A61C-665111F2D5E6 Etymology. We are pleased to name the new species to honour Riochi Takahashi, an outstanding Japanese aphidologist who has worked for several years on the aphid fauna of Taiwan and was the collector of the ...
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... alate viviparous females share similarly short and wide pterostigma of blunt edges and robust antennae (Figure 38(b)-(d)). Antennal sensilla in Sinolachnus are numerous and very protuberant (Figure 39(a)) which is very similar to characters of antennal sensilla of Protrama radicis (Figure 39 (b)) and E. moerickei (Figure 39(c)). Further, Sinolachnus, Protrama and Eotrama share the presence of secondary rhinaria on ANT V and ANT VI (Figure 39(d)-(f)). ...
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... alate viviparous females share similarly short and wide pterostigma of blunt edges and robust antennae (Figure 38(b)-(d)). Antennal sensilla in Sinolachnus are numerous and very protuberant (Figure 39(a)) which is very similar to characters of antennal sensilla of Protrama radicis (Figure 39 (b)) and E. moerickei (Figure 39(c)). Further, Sinolachnus, Protrama and Eotrama share the presence of secondary rhinaria on ANT V and ANT VI (Figure 39(d)-(f)). ...
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... alate viviparous females share similarly short and wide pterostigma of blunt edges and robust antennae (Figure 38(b)-(d)). Antennal sensilla in Sinolachnus are numerous and very protuberant (Figure 39(a)) which is very similar to characters of antennal sensilla of Protrama radicis (Figure 39 (b)) and E. moerickei (Figure 39(c)). Further, Sinolachnus, Protrama and Eotrama share the presence of secondary rhinaria on ANT V and ANT VI (Figure 39(d)-(f)). ...
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... sensilla in Sinolachnus are numerous and very protuberant (Figure 39(a)) which is very similar to characters of antennal sensilla of Protrama radicis (Figure 39 (b)) and E. moerickei (Figure 39(c)). Further, Sinolachnus, Protrama and Eotrama share the presence of secondary rhinaria on ANT V and ANT VI (Figure 39(d)-(f)). This is how a major difference between Sinolachnus and Tuberolachnini in the case of Tramini becomes a similarity -the position and arrangement of accessory rhinaria relative to the major rhinarium. ...
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... is how a major difference between Sinolachnus and Tuberolachnini in the case of Tramini becomes a similarity -the position and arrangement of accessory rhinaria relative to the major rhinarium. In Protrama (also in Trama) the secondary rhinaria, as in Sinolachnus, are in linear position and occur as singlular structures partly on the ANT VI BASE and spanning the PT (this character of ANT VI antennal sensilla is also present in Stomaphis) (Figure 39(d) and (e)). In this case, E. moerickei is the most outstanding case with secondary rhinaria rather as one group and also rather on the BASE (Figure 39(f)). ...
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... Protrama (also in Trama) the secondary rhinaria, as in Sinolachnus, are in linear position and occur as singlular structures partly on the ANT VI BASE and spanning the PT (this character of ANT VI antennal sensilla is also present in Stomaphis) (Figure 39(d) and (e)). In this case, E. moerickei is the most outstanding case with secondary rhinaria rather as one group and also rather on the BASE (Figure 39(f)). What is very noticeable is the very similar morphology of the first segments of especially fore and middle (Protrama and Trama) and all tarsi (Eotrama) with first segments of tarsi in Sinolachnus representatives. ...
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... is very noticeable is the very similar morphology of the first segments of especially fore and middle (Protrama and Trama) and all tarsi (Eotrama) with first segments of tarsi in Sinolachnus representatives. As in Sinolachnus (Figure 39(g)), Tramini members are characterized by short dorsal length and rounded distal part of the ventral side and numerous evident peg-like setae on middle and fore tarsi (Figure 39(h)-(l)). Apterous viviparous females of Sinolachnus are obligatory myrmecophilous as Tramini and they are well adapted to this relationship: they can live in soil shelters made by the ants and they have the same morphological adaptations in the form of the trophobiotic organ (Figure 2(b)-(e)) recently discovered in Tramini and almost the same as in Protrama ( Kanturski et al. 2017b;Kaszyca-Taszakowska & Yamamoto 2020). ...
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... is very noticeable is the very similar morphology of the first segments of especially fore and middle (Protrama and Trama) and all tarsi (Eotrama) with first segments of tarsi in Sinolachnus representatives. As in Sinolachnus (Figure 39(g)), Tramini members are characterized by short dorsal length and rounded distal part of the ventral side and numerous evident peg-like setae on middle and fore tarsi (Figure 39(h)-(l)). Apterous viviparous females of Sinolachnus are obligatory myrmecophilous as Tramini and they are well adapted to this relationship: they can live in soil shelters made by the ants and they have the same morphological adaptations in the form of the trophobiotic organ (Figure 2(b)-(e)) recently discovered in Tramini and almost the same as in Protrama ( Kanturski et al. 2017b;Kaszyca-Taszakowska & Yamamoto 2020). ...

Citations

... In general, Sinolachnus is clearly one of the most challenging Lachninae genera, and perhaps, this was the reason that in some cases, species from this genus have been described in other genera, such as Maculolachnus or Cinara Curtis, 1835. Kanturski et al. [23], during the revision of the genus, pointed out several differences between Sinolachnus from Tuberolachnini and similarities with Tramini, transferring it to the latter tribe. ...
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The tribe Tuberolachnini within the Lachninae (Hemiptera: Aphididae) is particularly intriguing due to its morphological traits and various ecological associations. Among the genera within this group, Pyrolachnus stands out as relatively understudied. Currently, only one species, Pyrolachnus imbricatus nipponicus Sorin, 2011, is known from Japan, distinguished by its distinctive characteristics. Through meticulous morphological analyses, we introduce a novel Lachninae genus, Miyalachnus gen. nov., associated with Cerasus and Prunus spp. (Rosaceae) in Japan. This new genus accommodates P. imbricatus nipponicus, now recognized as Miyalachnus nipponicus (Sorin, 2011) comb. nov. Additionally, we present a second species within this genus, Miyalachnus sorini sp. nov., along with comprehensive SEM morphological examination and insights into its biology. Our study describes in detail the morphological characteristics of both viviparous and bisexual generations of Miyalachnus, as well as their relationships with related genera.
... nov., they are relatively similar in some general features, which was also reflected in the phylogenetic results ( Figure 1). Additionally, the two new species differ from the micromeli/piri/xitianmushanus group of species in having different numbers of basal (16)(17)(18)(19)(20)(21), URS accessory (14)(15)(16), and ABD VIII setae (36)(37)(38)(39)(40)(41)(42), which in the micromeli/piri/xitianmushanus group are more numerous (20-27, 18-25, and 45-52, respectively). The last group of similar species, micromeli/piri/xitianmushanus, besides the already mentioned differences, is characterised by brown to dark pigmented distal parts of hind tibiae and antennal segments and their close relation in the results of the phylogenetic analyses ( Figure 1). ...
... One more species of Tuberolachnini-Pyrolachnus imbricatus nipponicus Sorin, 2011-also seems to be far from the other species of Pyrolachnus and Tuberolachnini, but in this case, several important differences were noted and placed the generic and tribal identity of this species in question. As P. imbricatus nipponicus shares some features with Sinolachnus yushanensis Kanturski et al. [14], those taxa formed one clade; however, this needs to be confirmed by molecular study, and this result should not question Sinolachnus as a member of Tramini [14]. Stomaphidini and root-feeding Tramini formed sister groups, as always in morphological investigations [41], probably due to a similar life mode (underground or often deep under the bark), despite the fact that Stomaphidini are the sister group to the clade formed by Tramini and Tuberolachnini in recent molecular results [1]. ...
... One more species of Tuberolachnini-Pyrolachnus imbricatus nipponicus Sorin, 2011-also seems to be far from the other species of Pyrolachnus and Tuberolachnini, but in this case, several important differences were noted and placed the generic and tribal identity of this species in question. As P. imbricatus nipponicus shares some features with Sinolachnus yushanensis Kanturski et al. [14], those taxa formed one clade; however, this needs to be confirmed by molecular study, and this result should not question Sinolachnus as a member of Tramini [14]. Stomaphidini and root-feeding Tramini formed sister groups, as always in morphological investigations [41], probably due to a similar life mode (underground or often deep under the bark), despite the fact that Stomaphidini are the sister group to the clade formed by Tramini and Tuberolachnini in recent molecular results [1]. ...
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Nippolachnus Matsumura, 1917 is a small aphid genus from the tribe Tuberolachnini (Hemiptera: Lachninae) occurring in Southeast Asia. Species from this genus are quite characteristic and stand out among lachnids for their morphology and ecological associations. We have performed a revision and phylogenetic analyses to elucidate the relationships within Nippolachnus and other representatives of Tuberolachnini. Here, the taxonomy of the genus is revised based on morphological data to include seven species, three of them newly described: Nippolachnus chakrabartii sp. nov. from India, Nippolachnus sinensis sp. nov. from China, and Nippolachnus malayaensis sp. nov. from Indonesia. Nippolachnus appear to be non monophyletic genus and a new genus, Indolachnus gen. nov., is described to accommodate Nippolachnus himalayensis (van der Goot, 1917) as Indolachnus himalayensis (van der Goot, 1917) comb. nov. The new genus is a sister group to the remaining Nippolachnus species, which created a monophyletic clade. Neonippolachnus Shinji, 1924 syn. nov. is recognised as a synonym of Nippolachnus, and Neonippolachnus betulae Shinji, 1924 syn. nov. as a synonym of Nippolachnus micromeli Shinji, 1924. For the first time, a scanning electron microscopy study of the sexual generation of N. piri Matsumura, 1917 has been performed. Apterous and alate viviparous females of N. bengalensis Basu and Hille Ris Lambers, 1968, N. piri, and N. micromeli, and alate viviparous females of N. xitianmushanus Zhang and Zhong, 1982 are re-described and illustrated, as well as apterous and alate viviparous females of I. himalayensis comb. nov. Hitherto unknown morphs of N. micromeli, N. piri, and N. xitianmushanus are described. A lectotype and paralectotypes of N. xitianmushanus are designated herein. Notes on distribution and host plants are given, and keys to apterous and alate viviparous females of the genera Nippolachnus and Indolachnus are also provided.
... Classical methods of species verification such a conventional light microscopy can be strengthened by detailed observations of body structures in SEM images. Evaluation of morphometric variation at the macro-and micro-morphological levels using SEM for taxonomic differentiation of closely related taxa of insects (including aphids) is increasingly used (Kanturski et al. 2015(Kanturski et al. , 2018a(Kanturski et al. , 2020(Kanturski et al. , 2023Mittné et al. 2022). Our SEM work with of Drepanaphis has resulted in morphological discernment and reveals new diagnostic features, such as the The combination of precision morphological comparisons at the macro-and microscopic levels along with DNA sequence data add to our understanding of species delimitation. ...
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The Nearctic genus Drepanaphis Del Guercio, 1909 currently includes 16 species with similar morphometric features, and three-dimensional structures may be important in species identification. The form (shape) of the dorsal abdominal tubercles, however, can be distorted by mounting on microscopic slides and this ultimately clouds diagnostic characters. This paper focuses on the identification of three species belonging to the genus Drepanaphis: Drepanaphis acerifoliae (Thomas, 1878), D. kanzensis Smith, 1941 and D. sabrinae Miller, 1937, to show the apparent differences of the structures of the examined individuals based on analysis of material deposited in museum collections and freshly collected material. To verify structural differences more precisely, we used Scanning Electron Microscopy to depict morphological characters accurately and DNA barcoding to analyze individuals at the molecular level.
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Two new Sinolachnus species from China, Sinolachnus rubusis Qiao & Li, sp. nov. feeding on Rubus sp. from Shaanxi and Sichuan Provinces, and Sinolachnus yunnanensis Qiao & Li, sp. nov. feeding on Elaeagnus sp. from Yunnan Province, are described and illustrated. Keys to Sinolachnus species distributed in China are presented. All examined specimens are deposited in the National Zoological Museum of China, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.