Figure - available from: Journal of Bacteriology
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SDS-PAGE of NIFL and NIFA tryptic peptides generated in the presence and absence of nucleotides. Incubations were carried out as described in the legend to Fig. 2, and the reactions were analyzed on 12.5% polyacrylamide gels. Peptides were generated by digestion with trypsin (weight ratio, 1:100) for 45 min at 20°C. (A) No nucleotide. (B) MgADP (1 mM). (C) MgATPγS (1 mM). (D) MgGTPγS. (E) Schematic representation of the NIFA peptide A7 protected in the presence of NIFL. Bands labeled A4 to -6 and L1 and -2 are shown in Fig. 1B and 2A, respectively. In each case, lane 1 is NIFL, lane 2 is NIFA, and lane 3 is NIFL plus NIFA.
Source publication
The enhancer binding protein NIFA and the sensor protein NIFL fromAzotobacter vinelandii comprise an atypical two-component regulatory system in which signal transduction occurs via complex formation between the
two proteins rather than by the phosphotransfer mechanism, which is characteristic of orthodox systems. The inhibitory activity
of NIFL to...
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In Azotobacter vinelandii, activation of nif gene expression by the transcriptional regulatory enhancer binding protein NIFA is controlled by the sensor protein NIFL in response to changes in levels of oxygen and fixed nitrogen in vivo. NIFL is a novel redox-sensing flavoprotein which is also responsive to adenosine nucleotides in vitro. Inhibition...
