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S3 Characterization of Treg cells during multiple TH1 infections. (A) BrdU incorporation by T-bet + Treg cells was measured following T. gondii infection by flow cytometry, LPL shown from naïve and day 9 infected mice, (gated on Foxp3 + , CD4 + , live/dead-cells). (B) Treg cell expression of Ki67 following oral T. gondii infection (gated on Foxp3 + , CD4 + , live cells), day 9 shown. (C-F) WT or DEREG mice were infected orally with 10 cysts of ME49. Two days postinfection, DT was administrated daily until day 9 to deplete CD4 + Foxp3 + Treg cells. (C) Treg cell frequencies in spleen, MLN and LP compartments at day 6 postinfection (day 4 of DT treatment). (D) Cells were stained for TCR-β, CD4, CD8, Foxp3, and Ki67 to determine effector T cell proliferation after Treg cell depletion.
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Interleukin (IL)–27 is a heterodimeric cytokine with potent inhibitory properties. Thus, mice that lack IL–27–mediated signaling develop exaggerated inflammatory responses during toxoplasmosis as well as other infections or autoimmune processes. While regulatory T (Treg) cells are critical to limit inflammation, their role during toxoplasmosis is c...
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