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Objective: Onosma echioides (OE) L., family Boraginaceae, is most recurrently used remedial herb, which is extensively dispersed all over India. It is used as purgative, bladder obstacle, liver chaos, kidney obstruction, sciatic pain, and gout. The study was meant to carry out the physicochemical, phytochemical, and high-performance thin layer chro...
Citations
... Mainly the root extracts of O. echioides are applied as antiseptic for skin injuries, contusion and eruptions. The two main active components namely shikonin and alkannin (enantiomeric natural dyes) along with their oligomers is present in O. echioides root bark (SHOAIB et al., 2017). Shikonin is the major component and possess wound healing, anti-cancer activity and has shown apoptosis and cell cycle arrest in MDA-MB-231 and HCT-116 cancer cell lines (Barreto et al., Kumar et 475 al., 2007, Kumar et al., 2013. ...
The present study is aimed at evaluation of phytosomal gel of the petroleum ether extract of root bark of Onosma echiodes for wound healing activity in rats. Extract of root bark of O. echioides was standardized by isolated naphthoquinone dimer using HPTLC. Phytosomes (equivalent to 2% w/w of naphthoquinones) of the standardized extract were prepared by thin film hydration technique. The wound healing efficacy of the formulation was evaluated in rats by inflicting excision and incision wounds followed by treatment of the wounds topically. The parameters evaluated for healing included determination of breaking strength and tensile strength of healed skin for incision model and percentage wound contraction, hydroxyproline content, granulation tissue free radicals and catalase in excision wound model. The formulation treated group showed a significant healing (p<0.005) of both the excision and incision wounds with respect to wound contraction and tensile strength respectively, as compared to vehicle treated group. The oxidative stress of the granulation tissue was also found to be reduced as indicated by reduced lipid peroxidation and increase in catalase activity. The phytosomal gel of O. echioides effectively exhibited wound healing effect.
... Phytochemical screening was useful to recognize the nature of the substance present in the various solvents (Pattnaik et al., 2013). The preliminary phytochemical screening demonstrated the presences of phenols, flavones, tannins, coumarins, saponins, alkaloids and glycosides (Shoaib et al., 2017). The preliminary phytochemical screening is basic for distinguishing proof of the distinctive phytoconstituents present in plant material (Koparde & Magdum, 2017). ...
Buchanania lanzan Spreng belongs to the family Anacardiaceae. The primary focus of this study was to examine phytochemical, proximate, antioxidant property and GCMS evaluation of unripe and ripen fruit of B. lanzan Spreng. The phytochemical studies showed that alkaloids, phenols, flavones, saponins, coumarins, glycosides and tannins are present in both ripen and unripe fruits. The proximate evaluation confirmed that crude fat (14.5%) and protein (6.37±0.69 g/100g) is high in ripen fruit. In unripe fruit (11.3%) crude fat and protein (4.11%) is less percent. The carotenoid (5.58 ± 0.5 mg/100g) and catalase (0.226±0.074 mg/100g) activity is higher in unripe fruit. The total polyphenol (6.4±0.8 mg/100g) and peroxidase (0.362±0.017 mg/100g) content show greater activity in ripen fruit. In DPPH and FRAP highest activity showed in methanol extract of ripen and unripe fruit than the other solvent. GC-MS evaluation showed many bioactive compounds present in unripe and ripen fruit. It is concluded that nutritional and bioactive ability is high in ripen and unripe fruits of B. lanzan. The fruits of B.lanzan are a good source of nutrition and medicinally important.
... The chromatograms were scanned by a CAMAG TLC Scanner after spraying with respective spray reagents and dried at 100° in a hot air oven. The peak number with its height, area and R f values of fingerprint data were recorded by WIN CATS (1.3.4 version) software [9][10][11][12][13][14]. ...
Objective: The objective of the study was to isolate the conditional amino acids present in the fresh leaves extracts of Alternanthera sessilis and compare the isolated amino acids with amino acid standards. Method: The fresh plant material was used for extraction. For the extracted sample solution, paper chromatography and high-performance thin-layer chromatography (HPTLC) fingerprinting analysis were then carried out. Results: Since A. sessilis is rich in protein, various amino acids have been isolated in aggregates and paper chromatography has been performed. Amino acids were separated and identified using trial and error method. Cysteine, glycine, aspartic acid, and proline are the standard amino acids used for comparing with the isolated amino acids by HPTLC fingerprint analysis, which gives an idea for the authentication of the plant, its constituents and also provides a parameter for quality control. Conclusion: The results scientifically validate the use of A. sessilis in the traditional medicine and also as a food.
