Reverse transcription reaction system

Reverse transcription reaction system

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Background The regulatory mechanisms underlying symbiotic nitrogen fixation between rhizobium and legumes remain unclear. Results In this study, integrated transcriptomics and root morphology analyses revealed that the inoculation of five rhizobial strains significantly increased root length, the number of tips, and nodule size in Mengnong clover N...

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Context 1
... utilizing the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, 18,007 unigenes (18.59 percent) were assigned to 140 pathways (Supplementary File 2). Table 1 Throughput and quality of RNA-seq of DGE libraries Sample Raw reads Raw bases Clean reads Clean bases Q20(percent) Q30(percent) GC ...
Context 2
... corresponding reaction system was established as shown in Table 2. The reverse transcription process was carried out under the following conditions: 42°C for 30 minutes followed by a denaturation step at 85°C for 5 seconds. ...

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