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Results of H. heilmannii polymerase chain reaction (PCR) detection. Lane 1: 100 bp ladder, lane 2: negative control, lane 3: positive control (580 bp), lanes 5 and 6: positive sample, lanes 4, 7 and 8: negative samples
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Background
Companion animals like dogs play an important role in the lives of many people and are often considered to be members of families, but definitely, any contact with them poses an inherent risk of transmitting zoonotic pathogens. One of these pathogens is the genus Helicobacter which is linked to many disorders in human and animal.
Object...
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Background
Reports on eradication of non-Helicobacter pylori Helicobacter (NHPH) gastritis in Japanese patients are rare. We investigated effectiveness of the standard triple-drug combination therapy for Helicobacter pylori eradication and of proton pump inhibitor (PPI) monotherapy in eradication of non-Helicobacter pylori Helicobacter.
Methods
Sub...
Citations
... Although gastroscopy, which is within the invasive diagnostic methods, has higher sensitivity and specificity than other methods, it should be noted that many veterinarians do not have the opportunity and ability to perform gastroscopy, in addition to the risks of gastroscopy and associated anaesthesia. For this reason, non-invasive methods such as serology, culture or PCR, which can be made from blood, saliva and faecal samples, are preferred [6]. In addition, it was reported in a study that PCR analyses alone would not be sufficient to associate the presence of bacteria with the disease state in the diagnosis of Helicobacter, and this should be supported by histopathological analyses [7]. ...
In order to determine the presence of Helicobacter in shelter Dogs in Yozgat Province of Türkiye by fecal antigen test and ELISA and to treat the infection during the initial period. As the material of the study, faecal antigen test, faecal samples for ELISA, blood samples for haematological and biochemical analyses were collected from 82 dogs in Sorgun animal shelter where food and water bowls were kept in compartments. Infected and control groups were formed according to the results of the fecal antigen test. As a result of fecal antigen test, 6 of 82 animals were antigen positive and 76 were antigen negative. According to ELISA analysis; 53 dogs were antibody positive and 29 dogs were antibody negative. No significance was determined between the groups in terms of age and gender in both tests (P>0.05). Dogs in the infected group were treated according to a protocol known as triple therapy, which is used in Helicobacter infections. WBC, NEU and MON counts, RDW and % NEU values of the infected group were higher in the hematological examination, while the % LYM values were significantly lower than the control group. In the biochemical examination, the concentrations TNF–α (21.17 pg·mL-1 vs. 48.21 pg·mL-1), IL–1β (73.41 pg·mL-1 vs. 37.60 pg·mL-1) (P<0.01) and CRP (644.0 mg·dL-1 vs. 234.01 mg·dL-1) (P<0.001) were found to be higher than those of the control group. As a result, it was determined in the study that the presence of Helicobacter was intense in dogs that were in one–to–one contact with each other, including the feeding of dogs in shelter conditions. With the methods used in the study, it is predicted that the presence of Helicobacter can be detected and treated in the early stages, thus preventing transmission of Helicobacter in dogs.
... Due to the detection of two Helicobacter spp. (Helicobacter heilmannii and Helicobacter bilis), improving the owners' knowledge of this finding is important, regardless of the minor role of fecal-oral transmission in the particular study 12 . ...
... Incidentally, H. pylori was the second most prevalent (26.39%) species detected, unlike H. felis, H. bizzozeronii, and H. heilmannii s.s. often highlighted as the predominant GH in canines from some other studies[28]. In contrast, however, a high prevalence of H. pylori infection (62.5% and 41.43%) in canine stool samples from Egypt has been reported[29,30]. ...
Background. It is probable that humans can acquire H. pylori and non-H. pylori Helicobacter infections via domestic animals. The prevalence and risk factors of infections of Helicobacter species in canines of Taipei city were therefore analysed in this study. Materials and methods. A total of 95 canine faecal samples were collected from different animal shelters and hospitals in Taipei city. Total DNA was extracted for semi-nested PCR detection of Helicobacter species. The PCR products were sequenced for further comparative database and phylogenetic analyses. Results. The overall prevalence of Helicobacter species in canines of Taipei city was 75.79% (72/95). Two gastric, seven enterohepatic and two unclassified Helicobacter species were identified, all of which have been implicated in the aetiology of human diseases. The predominant species detected included H. canis (27.78%), H. pylori (26.39%), H. canicola (18.06%), and H. bilis (13.89%) in decreasing order, while H. canadensis and H. typhlonius were identified for the first time in canines. The genotypes in H. pylori and H. canicola clusters grouped together, with their respective reference strains, showed a close evolutionary distance in the phylogenetic tree, indicating a common ancestry may have existed in these clusters respectively. The residential region of canines, dog living status (pet or stray) and breed (purebred or mixed-breed) are the risk factors associated with Helicobacter infections in the canines examined. Conclusion. The high prevalence of Helicobacter infections in canines highlights a potential public health risk of zoonotic transmission among dogs, humans and other animals, and therefore, the need for proper methods in controlling the transmission routes. In addition, the 16S rRNA gene amplification method was found to be useful for bacterial identification and phylogenetic analysis.
... Incidentally, H. pylori was the second most prevalent (26.39%) species detected, unlike H. felis, H. bizzozeronii, and H. heilmannii s.s. often highlighted as the predominant GH in canines from some other studies [28]. In contrast, however, a high prevalence of H. pylori infection (62.5% and 41.43%) in canine stool samples from Egypt has been reported [29,30]. ...
Background
It is probable that humans can acquire H. pylori and nonH. pylori Helicobacter infections via domestic animals. The prevalence and risk factors of infections of Helicobacter species in canines of Taipei city were therefore analysed in this study.
Materials and methods
A total of 95 canine faecal samples were collected from different animal shelters and hospitals in Taipei city. Total DNA was extracted for semi-nested PCR detection of Helicobacter species. The PCR products were sequenced for further comparative database and phylogenetic analyses.
Results
The overall prevalence of Helicobacter species in canines of Taipei city was 75.79% (72/95). Two gastric, seven enterohepatic and two unclassified Helicobacter species were identified, all of which have been implicated in the aetiology of human diseases. The predominant species detected included H. canis (27.78%), H. pylori (26.39%), H. canicola (18.06%), and H. bilis (13.89%) in decreasing order, while H. canadensis and H. typhlonius were identified for the first time in canines. The genotypes in H. pylori and H. canicola clusters grouped together, with their respective reference strains, showed a close evolutionary distance in the phylogenetic tree, indicating a common ancestry may have existed in these clusters respectively. The residential region of canines, dog living status (pet or stray) and breed (purebred or mixed-breed) are the risk factors associated with Helicobacter infections in the canines examined.
Conclusion
The high prevalence of Helicobacter infections in canines highlights a potential public health risk of zoonotic transmission among dogs, humans and other animals, and therefore, the need for proper methods in controlling the transmission routes. In addition, the 16S rRNA gene amplification method was found to be useful for bacterial identification and phylogenetic analysis.
