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To compare sciatic nerve regeneration in rats using three different techniques of repair.
Fifteen isogonics rats were divided into three groups according to the method used to repair a 5-mm long defect created in the sciatic nerve: autogenous graft (Group A), polyglycolic acid tube (PGAt) (Group B), and of the association of PGAt with the graft (Gr...
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The regenerative capability of peripheral nerves is very limited, and several strategies have been proposed to increase nerve regeneration. In the present work, we have analyzed the in vivo usefulness of a novel nanostructured fibrin-agarose bio-artificial nerve substitute (Nano) used alone or in combination with NeuraGen® collagen type I conduits...
Background
We compared different surgical techniques for nerve regeneration in a rabbit sciatic nerve gap model using magnetic resonance diffusion tensor imaging (DTI), electrophysiology, limb function, and histology.
Methods
A total of 24 male New Zealand white rabbits were randomized into three groups: autograft (n = 8), hollow conduit (n = 8),...
Objective: The physical properties of nerve guidance channel and components of the regenerating microenvironment can significantly enhance regeneration. The aim of this research was to evaluate the effect of embryo cerebrospinal fluid (ECSF) in nerve regeneration across the microwave irradiated collagen nerve guides in comparison with autograft.
Ma...
Background/aim:
The aim of this study was to compare electrospun caprolactone (EC) and poly(lactic acid-ε-caprolactone) (PLCL) nerve conduits with nerve graft in a rat sciatic nerve defect model.
Materials and methods:
A total of 32 male Wistar albino rats were divided into 4 groups, with 8 rats in each group. A nerve defect of 1 cm was construc...
Objective: The physical properties of nerve guidance channel and components of the regenerating microenvironment can significantly enhance regeneration. The aim of this research was to evaluate the effect of embryo cerebrospinal fluid (ECSF) in nerve regeneration across the microwave irradiated collagen nerve guides in comparison with autograft.
Ma...
Citations
... Багато біоматеріалів випробовувалося у експериментах [42], найбільш обнадійливі результати були досягнуті за допомогою полігліколевої кислоти (ПГК). Була показана здатність полігліколевих трубок покращувати регенерацію ПН при його критичних дефектах, як у експерименті [43], так і у клініці [44]. ...
Peripheral nerve injuries are significant problem in the medical and socio-economic plan, as they are accompanied by a high incidence of disability by people of working age.
In recent decades, significant progress has been made in the restorative surgery of the peripheral nervous system, in particular through the introduction into clinical practice of microsurgical techniques. However, the problem of restoring the peripheral nerve after its traumatic injury has not been resolved yet.
Review article addresses the current state of developing stem cell technologies for peripheral nerve repair. Basic concepts of peripheral nerve regeneration after traumatic injury, methods of their restoration in experimental and clinic conditions are considered. The prospect of using stem cells of different origins is shown in the experiment by many authors, and the positive effect of stem cells on peripheral nerve regeneration is explained by their ability to secrete many trophic factors and differentiation to a neural phenotype. An essential issue in the tissue engineering approach is the choice of the optimal material to be used as a scaffold for large size peripheral nerve defects grafting.
The article focuses on the main types of stem cells, as well as their combinations with biopolymers, which have shown efficiency in the experiment. Despite the advances in the use of the latest technologies, the search for the necessary components is underway to provide the most favorable conditions for peripheral nerve regeneration in the clinic.
... In terms of the current state of the art, there are typically two approaches to peripheral nerve regeneration: cell-free tissue-engineered nerve grafts and cell based therapeutic approaches. Taking the carrier in isolation, morphologically, the cell free NGC performed well in comparison to recent studies over similar defect distances on other biomaterials [31,32]. The axons generated by the NGC in this study gave better results in terms of total axon count (NGC gave a mean axon count of 4,671 compared to Costas [30] reported 4,225.2 or de Ruiters [32] reported 2,925 for a PGLA multichannel tube) and critically, axonal calibre (3.6 mm compared to de Ruiters 4.01). ...
Peripheral nerve injury presents significant therapeutic challenges for recovery of motor and sensory function in patients. Different clinical approaches exist but to date there has been no consensus on the most effective method of treatment. Here, we investigate a novel approach to peripheral nerve repair using olfactory derived stem (ONS) cells delivered in a biphasic collagen and laminin functionalized hyaluronic acid based nerve guidance conduit (NGC). Nerve regeneration was studied across a 10-mm sciatic nerve gap in Sprague Dawley rats. The effect of ONS cell loading of NGCs with or without nerve growth factor (NGF) supplementation on nerve repair was compared to a cell-free NGC across a variety of clinical, functional, electrophysiological, and morphologic parameters. Animals implanted with ONS cell loaded NGCs demonstrated improved clinical and electrophysiological outcomes compared to cell free NGC controls. The nerves regenerated across ONS cell loaded NGCs contained significantly more axons than cell-free NGCs. A return of the nocioceptive withdrawal reflex in ONS cell treated animals indicated an advanced repair stage at a relatively early time point of 8 weeks post implantation. The addition of NGF further improved the outcomes of the repair indicating the potential beneficial effect of a combined stem cell/growth factor treatment strategy delivered on NGCs. Stem Cells Translational Medicine 2017;6:1894–1904
... 12,13 Synthetic conduits built from absorbable materials such as polyglycolic acid and poly-3hydroxybutyrate (PHB) have also been tested and had results similar to those of the standard autograft treatment. 14,15 However, there is still no biological or synthetic conduit available for clinical use that has the same regenerative capacity as an autologous nerve graft. ...
IntroductionThe standard treatment for nerve defects is nerve autograft. There is no conduit available that provides the same regenerative capacity of nerve autograft. This study evaluated the histological and functional recovery of nerve defects treated with fibrin conduit in comparison to the nerve autograft, in a rat model.MethodA sciatic nerve injury model (10-mm defect) was performed in 20 Wistar rats, nerve defect was reconstructed using a fibrin conduit (n = 10). A nerve autograft was used as control (n = 10). The walking behavior was measured by footprint analysis at 4, 8, and 12 weeks and sciatic function index was determined. After 12 weeks, histological analysis was performed to evaluate the regenerated nerve and measured axonal density. The triceps surae muscle weight was also evaluated.ResultsThe fibrin conduit group showed less improvement in walking behavior compared to nerve autograft (−53 ± 2 vs. −36 ± 2; P < 0.001 at 12 weeks). The fibrin conduit group presented axonal density of 40.0 axons/10.995μm2 and the nerve autograft group had 67.2 axons/10.995μm2 (P < 0.001). The triceps surae muscle weight ratio of the fibrin conduit group was 41 ± 3% versus 71 ± 4% of the nerve autograft group (P < 0.001).Conclusion
The fibrin conduit could be used for nerve reconstruction following peripheral nerve injury in the rat model. However, the functional recovery in the fibrin conduit repair group was worse than that in nerve autograft group and the nerve repair with the fibrin conduit has less myelinated fibers when compared to the repair with nerve autograft. © 2015 Wiley Periodicals, Inc. Microsurgery, 2015.
Background:
Centella asiatica (L.) is a plant with neuroprotective and neuroregenerative properties; however, its effects on the neurodifferentiation of mesenchymal stem cells (MSCs) and on peripheral nerve injury are poorly explored. This study aimed to investigate the effects of C. asiatica (L.)-neurodifferentiated MSCs on the regeneration of peripheral nerve in a critical-size defect animal model.
Methods:
Nerve conduit was developed using decellularised artery seeded with C. asiatica-neurodifferentiated MSCs (ndMSCs). A 1.5 cm sciatic nerve injury in Sprague-Dawley rat was bridged with reversed autograft (RA) (n = 3, the gold standard treatment), MSC-seeded conduit (MC) (n = 4) or ndMSC-seeded conduit (NC) (n = 4). Pinch test and nerve conduction study were performed every 2 weeks for a total of 12 weeks. At the 12th week, the conduits were examined by histology and transmission electron microscopy.
Results:
NC implantation improved the rats' sensory sensitivity in a similar manner to RA. At the 12th week, nerve conduction velocity was the highest in NC compared with that of RA and MC. Axonal regeneration was enhanced in NC and RA as shown by the expression of myelin basic protein (MBP). The average number of myelinated axons was significantly higher in NC than in MC but significantly lower than in RA. The myelin sheath thickness was higher in NC than in MC but lower than in RA.
Conclusion:
NC showed promising effects on nerve regeneration and functional restoration similar to those of RA. These findings revealed the neuroregenerative properties of C. asiatica and its potential as an alternative strategy for the treatment of critical size nerve defect.
