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Representatives of Mediterranean to C. European Ranunculi and allied genera. A, Ranunculus thora (alpine limestone scree, Alps Maritimes); B, R. seguieri (alpine limestone scree, Alps Maritimes); C, Ceratocephala falcata (open places in cultivated land, W. Turkey; D, R. millefoliatus (open places in pastures, Sicily); E, R. aduncus (subalpine grassland, Alps Maritimes); F, R. cortusifolius ( Laurus forest, Tenerife). Photo credit: Franz Hadacek.
Source publication
Ranunculus s.l. shows a considerable species diversity and degree of endemism in the Mediterranean region and occurs with various life forms from the lowlands to the highest mountains. Based on a sampling from all continents, sequences of the ITS of nrDNA, the plastid matK, and the adjacent trnK regions were analysed using maximum parsimony and Bay...
Contexts in source publication
Context 1
... & Cook, 1993, in Flora Europaea), and the monotypic sections Acetosellifoli (Iberian peninsula), Physophyllum (omni- mediterranean), and Chloeranunculus (extending to Central France) sensu Tamura (1995). Altogether, Tamura's (1995) classification reflects a high morpho- logical diversity of buttercups in the Mediterranean region (see examples in Fig. ...
Context 2
... allopolyploid taxa of the R. auricomus complex, and several taxa from the southern hemisphere). Almost all ITS sequences were taken from Hörandl & al. (2005), whereas all plastid sequence data (except for the out- group) are newly reported here (Appendix 1). According to the delimitation of the Mediterranean region after Blondel & Aronson (1999: fig. 1.5), we include here 30 species endemic or subendemic to the Mediterranean region and one endemic to Macaronesia, plus 47 species non-endemic occurring in the region. Materials, vouch- ers, and GenBank accession numbers are documented in Appendix 1. Selection of Trautvetteria as an outgroup, which is the closest relative to the ...
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The Balkan Peninsula, characterized by high rates of endemism, is recognised as one of the most diverse and species-rich areas of Europe. However, little is known about the origin of Balkan endemics. The present study addresses the phylogenetic position of the Balkan endemic Ranunculus wettsteinii, as well as its taxonomic status and relationship w...
Premise of the study:
Oceanic island endemics typically exhibit very restricted distributions. In Macaronesia, only one endemic angiosperm species, Ranunculus cortusifolius, has a distribution spanning the archipelagos of the Azores, Madeira, and Canaries. Earlier work suggested possible differences between archipelagos and the multiple origins of...
Citations
... Ranunculus L. is the largest genus of Ranunculaceae and one of the 50 largest angioperm genera, with a worldwide distribution and around 600 herbaceous species (Tamura 1995, Frodin 2004, Cires et al. 2014). The majority of species are found in temperate to arctic/ subantarctic zones; nonetheless, the genus exhibits significant variety in the Mediterranean (Paun et al. 2005). In our country, Ranunculus species are recognized by names such as buttercup, oil bowl, and mule shoe (Fafal Erdogan and Yonter 2009). ...
The goal of this study is to conduct a thorough karyotypic examination of nine Ranunculus species [R. brachylobus subsp. incisilobatus, R. kotschyi (B1), R. kotschyi (B2), R. macrorrhynchus subsp. trigonocarpus, R. polyanthemos, R. repens, R. sericeus, R. sphaerospermus, and R. strigillosus] growing in Turkey. This study discovered that the basic number of Ranunculus taxa investigated was x=7 or 8. R. sphaerospermus and R. strigillosus have median and submedian chromosomes while other taxa have subterminal (st) chromosomes as well median (m) and submedian (sm). This investigation also revealed that R. kotschyi (B1), R. kotschyi (B2), and R. macrorrhynchus subsp. trigonocarpus are hexaploid, whereas R. repens and R. sphaerospermus are tetraploid. The Stebbins classification, ploidy levels, karyotype formula, chromosomal length range, total karyotype length, various karyotype asymmetry values, and the A1 and A2 in each taxon were also determined in this study. The Stebbins classification of R. brachylobus subsp. incisilobatus, R. polyanthemos, and R. repens were 3A while the other six taxa have 3B.
... However, this treatment was not supported by molecular phylogenetic analysis [45,46]. A large number of molecular phylogenetic studies of Ranunculeae have been published [11][12][13][47][48][49] which helped us understand the delimitation and generic relationship of this tribe. Based on molecular phylogeny and comprehensive sampling, 19 genera were recognized within the tribe Ranunculeae [11][12][13]. ...
The tribe Ranunculeae, Ranunculaceae, comprising 19 genera widely distributed all over the world. Although a large number of Sanger sequencing-based molecular phylogenetic studies have been published, very few studies have been performed on using genomic data to infer phylogenetic relationships within Ranunculeae. In this study, the complete plastid genomes of nine species (eleven samples) from Ceratocephala, Halerpestes, and Ranunculus were de novo assembled using a next-generation sequencing method. Previously published plastomes of Oxygraphis and other related genera of the family were downloaded from GenBank for comparative analysis. The complete plastome of each Ranunculeae species has 112 genes in total, including 78 protein-coding genes, 30 transfer RNA genes, and four ribosomal RNA genes. The plastome structure of Ranunculeae samples is conserved in gene order and arrangement. There are no inverted repeat (IR) region expansions and only one IR contraction was found in the tested samples. This study also compared plastome sequences across all the samples in gene collinearity, codon usage, RNA editing sites, nucleotide variability, simple sequence repeats, and positive selection sites. Phylogeny of the available Ranunculeae species was inferred by the plastome data using maximum-likelihood and Bayesian inference methods, and data partitioning strategies were tested. The phylogenetic relationships were better resolved compared to previous studies based on Sanger sequencing methods, showing the potential value of the plastome data in inferring the phylogeny of the tribe.
... Ranunculus L., with ca. 600 species, is the largest genus in the Ranunculaceae and is widely distributed in all continents (Tamura 1995;Hörandl et al. 2005;Paun et al. 2005;Hörandl and Emadzade 2012). More than 150 species and 30 varieties of Ranunculus are currently recognized in China, one of the centers of species diversity for the genus (Wang 1995a, b, Fei. ...
Ranunculus luanchuanensis (Ranunculaceae), a new species from Laojun Shan in Luanchuan county, Henan province, central China, is here illustrated and described. It is morphologically similar to R. limprichtii in having 3-lobed and subreniform basal leaves, 3-lobed cauline leaves, and small flowers with reflexed and caducous sepals, but differs by having slender and basally slightly thickened roots (vs. fusiform), prostrate stems (vs. erect), obliquely ovoid and glabrous carpels and achenes (vs. widely ovoid and puberulous), longer styles in the carpels (ca. 1.2 mm vs. 0.6–0.8 mm) and achenes (ca. 1.8 mm vs. 0.6–0.8 mm), and glabrous receptacles (vs. sparsely puberulous). Ranunculus luanchuanensis , currently known only from its type locality, is geographically isolated from R. limprichtii , a species widely distributed in Gansu, Qinghai, Sichuan, Xizang (Tibet) and Yunnan, China. The distribution map of this new species and its putative closest ally, R. limprichtii , is also provided.
... Ranunculus L., comprising approximately 600 species, is the largest genus in the Ranunculaceae and is widely distributed in all continents (Tamura 1995;Hörandl et al. 2005;Paun et al. 2005;Hörandl and Emadzade 2012). In China, one of the centers of species diversity in Ranunculus, more than 150 species and 30 varieties are currently recognized in the genus (Wang 1995a(Wang , b, 1996(Wang , 2007(Wang , 2008(Wang , 2013(Wang , 2015(Wang , 2016(Wang , 2018(Wang , 2019a(Wang , b, 2022Yang 2000;Wang and Gilbert 2001;Wang and Liao 2009;Luo and Zhao 2013;Wang and Chen 2015;Wang et al. 2016;Yuan and Yang 2017a, b, c;Zhang et al. 2020;Fei et al. 2022Fei et al. , 2023a. ...
Ranunculus maoxianensis (Ranunculaceae), a new species from Jiuding Shan in Maoxian county, northwestern Sichuan province, China, is here illustrated and described. The species is morphologically similar to R. chongzhouensis , a species also occurring in Sichuan, in having reniform leaves and puberulous receptacles, carpels and achenes, but differs by having leaves adaxially puberulous with shorter appressed hairs 0.16‒0.28 mm long (vs. longer appressed hairs 0.55‒0.85 mm long), larger flowers (1.8‒2 cm vs. 1.4‒1.6 cm in diameter), larger (8‒10 × 5.5‒6.5 mm vs. 6‒7 × 4.5‒5 mm) and widely obovate petals (vs. obovate), more numerous stamens (35‒55 vs. 12‒18), and subglobose gynoecium and aggregate fruit (vs. ellipsoid). The two species are also different in chromosome number and chromosome morphology. Ranunculus chongzhouensis has a karyotype of 2 n = 2 x = 16 = 10m + 6sm while R. maoxianensis has a karyotype of 2 n = 4 x = 32 = 16m + 16sm. An emended description of R. chongzhouensis is provided, and its geographical distribution is largely extended.
... Ranunculus L., comprising approximately 600 species, is the largest genus in the Ranunculaceae and is widely distributed in all continents (Tamura 1995;Paun et al. 2005;Hörandl and Emadzade 2012). In China, one of the centers of species diversity of Ranunculus, more than 150 species and 30 varieties are currently recognized in the genus (Wang 1995a(Wang , b, 1996(Wang , 2007(Wang , 2008(Wang , 2013(Wang , 2015(Wang , 2016(Wang , 2018(Wang , 2019a(Wang , b, 2022Yang 2000;Wang and Gilbert 2001;Wang and Liao 2009;Luo and Zhao 2013;Wang and Chen 2015;Wang et al. 2016;Yuan and Yang 2017a, b, c;Zhang et al. 2020;Fei et al. 2022Fei et al. , 2023a. ...
Ranunculus jiguanshanicus (Ranunculaceae), a new species from Chongzhou in Sichuan province, China, is here described and illustrated. The new species is easily distinguishable from other Chinese members of the genus by an array of characters, including small stature, glabrous and prostrate stems, 3-foliolate leaves with obvious petiolules (3–5 mm long), unequally 3-sected leaflets, lanceolate to linear ultimate leaflet segments, small flowers (5.2–6 mm in diameter), and long styles in the carpels and achenes (ca. 0.8 mm long). A distribution map of this new species is also provided.
... 600 genetically diverse species (Tamura 1995). The genus is distinguished by its high ecological-geographical diversity over a wide zonal spectrum ranging from the Arctic tundra through varied forests, steppes, deserts to exclusively aquatic habitats and high-altitude communities on nearly all continents (Paun et al. 2005). However, the main centres of speciation of Ranunculus are often in large mountain systems, where the formation of species is not only directly related to isolation, but also significantly depends on specific conditions of the highlands (Emadzade et al. 2015;Fernández Prieto et al. 2017;Shchegoleva 2018;Shchegoleva et al. 2020;Zverev et al. 2020). ...
New data on the phylogeny of four rare and endemic species of Ranunculus L. (sect. Ranunculastrum DC.) of western Pamir-Alai, one of which is new to science, have been obtained. Ranunculus tojibaevii sp. nov., from the Baysuntau Mountains, Western Hissar Range of Uzbekistan, is described. The new species is closely related to R. botschantzevii Ovcz., R. convexiusculus Kovalevsk. and R. alpigenus Kom., but differs in the blade of the radical leaves, which is rounded-reniform, segments 3-5-dissected, each 2-5-partite with elongated, rounded apical lobes. A phylogenetic analysis, using both the nuclear ribosomal internal transcribed spacer (ITS) and cpDNA (matK, rbcL, trnL-trnF), was informative in placing R. tojibaevii in context with its most closely-related species. Discussion on the geographic distribution, updated identification key, a detailed description, insights about its habitat and illustrations are provided.
... The occurrence of Aconitum in Central Europe can be traced back to as early as the Late Miocene, as suggested by the Aconitum pollen deposits found in the Central Paratethys realm (Central Europe) (Stuchlik & Shatilova, 1987). The Caucasian and European lines diverged in the Late Miocene, and internally diversified mainly in Table 2 and Table S1. the Quaternary, similarly to Ranunculus s. s. (Paun et al., 2005), Syringa (Kim & Jensen, 1998), and Wulfenia (Surina et al., 2014), highlighting the significance of this period for the evolution of the European mountain and high-mountain flora. ...
Phylogenetic relations within Aconitum subgen. Aconitum (Ranunculaceae) in Europe are still unclear. To infer the phylogeny of the nuclear (ITS) region and chloroplast intergenic spacer trnL (UAG)-ndhF of the chloroplast DNA (cpDNA), we analyzed 64 accessions within this taxon, 58 from Europe and six from the Caucasus Mts. Nuclear ITS sequences were identical in 51 European and two Caucasian accessions, whereas the remaining sequences were unique. cpDNA sequences could be categorized into five haplotypes, i.e., A-E, including a European-Caucasian Aconitum haplotype B. Ten cpDNA sequences were unique. A 5-bp indel distinguished the diploids from the tetraploids. None of the extant European diploids were basal to the tetraploid local group. A phylogenetic tree based on combined ITS and cpDNA sequences (bayesian inference, maximum likelihood, minimal parsimony) placed Aconitum burnatii (Maritime Alps, Massif Central) and A. nevadense (Sierra Nevada, Pyrenees) in a sister group to all other European species. A Bayesian relaxed clock model estimated the earliest split of the Caucasian species during the Late Miocene [ca. 7 million years ago (Mya)], and the divergence of A. burnatii and A. nevadense from the European genetic stock during the Miocene/Pliocene (ca. 4.4 Mya). Diploids in Europe are likely to be descendants of the Miocene European-Caucasian flora linked with the ancient Asian (arctiotertiary) genetic stock. The origins of the tetraploids remain unclear, and it is possible that some tetraploids originated from local, now extinct diploids. Both the diploids and tetraploids underwent rapid differentiation in the Late Pliocene-Quaternary period.
... Soon thereafter, DNA markers were implemented to clarify and discuss the phylogenetic relationships within Ranunculaceae [5][6][7][8]. Several publications using molecular markers have been dedicated to the Ranunculus genus and suggest that the molecular taxonomy of the genus is not congruent with the classifications based on botanical studies [8][9][10]. For instance, phylogenetic analyses of c. 200 species of Ranunculus based on sequences of the muclear ITS (nrITS) region [9] showed a high level of similarity to previous chloroplast DNA restriction site analyses [8] and differed from the previous classification based on phenetic studies. ...
... DNA barcoding studies have often been used by different scientists for evaluating the generic delimitation and infrageneric classification of the genus Ranunculus [9][10][11][12][13][14][15]. Most of these studies have relied on the use of nucleotide sequences of the internal transcribed spacer (ITS) of the nuclear ribosomal DNA (rDNA) region, as well as plastid regions. ...
Worldwide, the genus Ranunculus includes approximately 600 species and is highly genetically diverse. Recent taxonomic reports suggest that the genus has a monophyletic origin, divided into two subgenera, and consists of 17 sections. The Central Asian country of Kazakh-stan has 62 species of the genus that have primarily been collected in the central part of the country. The latest collection trips in southern parts of the country have led to the description of a wider distribution area for Ranunculus and the identification of a new species Ranunculus talassicus Schegol. et A.L. Ebel from Western Tien Shan. Therefore, in this study, attempts were made to assess the molecular taxonomic positions of R. talassicus and two other species endemic to the Central Asian region R. karkaralensis Schegol. and R. pskemensis V.N. Pavlov in relation to other species of the genus, using internal transcribed spacer (ITS) molecular genetic markers. The ITS-aligned sequences of 22 local Central Asian accessions and 43 accession sequences available in the National Center for Biotechnology Information (NCBI) database allowed the construction of a maximum parsimony phylogenetic tree and a Neighbor-Net network. The results indicated that R. talassicus and R. pskemensis could be assigned to section Ranunculastrum. Additionally, an assessment of the network suggested that R. pskemensis was the rooting taxon for the group of species containing R. talassicus, and that R. illyricus L. and R. pedatus Waldst. & Kit. were founders of a prime rooting node for the Ranunculastrum section of the genus. The ITS-aligned sequences showed that R. karkar-alensis was indifferent with respect to three other species in the Ranunculus section of the genus, i.e., R. acris L., R. grandifolius C.A. Mey., and R. subborealis Tzvelev. The study indicated that the assessments of ITS-based phylogenetic tree and Neighbor-Net network provided new insights into the taxonomic positions of three endemic species from Central Asia.
... (Ranunculaceae) is known for its great morphological polymorphism and the subsequent confusion created by the use of different synonymies (Ficaria or Ranunculus L. etc.). Molecular data support the discrimination of Ficaria and Ranunculus into two different genera (Paun & al. 2005). ...
A karyosystematic study of six interesting taxa from the Ionian Islands (Greece) is presented. The chromosome number (2n = 16) and karyotype analysis for two ecologically different populations of the Greek endemic Allium callimischon subsp. callimischon are given and its kary-otype variation is also confirmed. The chromosome number (2n = 16) and karyotype morphology of Allium flavum subsp. tauricum is reported for the first time in material from Ionian Islands. Karyotype analysis of Ficaria calthifolia (2n = 16) is given for first time in Greek material. Chromosome data (2n = 22) for Freesia leichtlinii subsp. alba are presented for the first time for this taxon. Previous karyological references of Paeonia mascula subsp. russoi (2n = 10) confirm our findings from a new population from north Kefallinia isl. The first karyolog-ical attempt at Romulea bulbocodium in Greece is performed here, resulting in two ploidy levels (2n = 3x = 33 and 2n = 4x = 44) for, ecologically, different populations and additionally the basic chromosome number of the genus is discussed.
... Furthermore, a host plant range that is wider than most other Pseudodineura species is already well documented for fuscula. According to Paun et al. (2005), the ranunculus species which we list as its hosts, excluding auricomus and two other species not included in that work (cassubicus, kerneri) belong to four main lineages, of which lineages I and VIII are the most distantly related within the ranunculus core clade. Currently, the only apparent taxonomic uncertainty affecting West Palaearctic Pseudodineura is whether specimens of P. fuscula feeding on ranunculus auricomus agg. ...
Six valid species of Pseudodineura are now recognised as occurring in the West Palaearctic, and the only described species of the related genus Endophytus. Larvae of all species are leaf-miners in Ranunculaceae. An identification key to adults is provided, followed by species commentaries which include summarised data on taxonomy, larval host plants, and distribution, with particular reference to Sweden. Whereas identification of some specimens using morphological characters may not be possible, each species apparently has a distinct COI barcode sequence. Pseudodineura heringi (Enslin, 1921) is a new junior synonym of P. parvula (Klug, 1816). Pseudodineura mocsaryi Zombori, 1976 and P. scaligera Zombori, 1979 are new junior synonyms of P. clematidisrectae Hering, 1935. Lectotypes are designated for: Dolerus minutus Hartig, 1837, Pelmatopus clematidis Hering, 1932, P. enslini Hering, 1923, P. heringi Enslin, 1921, and P. mentiens var. konowi Enslin, 1921.
