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Insights into the eukaryotic gene regulation networks have improved due to the advent of diverse classes of non-coding RNAs. The transfer RNA (tRNA)-derived non-coding RNAs or tncRNAs is a novel class of non-coding RNAs, shown to regulate gene expression at transcription and translation levels. Here, we present a pipeline ‘tncRNA Toolkit’ for accur...
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Citations
... Building an artificial tRNA genome to map tRNA sequencing reads is also the solution proposed by tReasure (Lee et al., 2022), a package to analyze tRNA expression profiles to be used in R (Giorgi et al., 2022). Similarly, MINTmap was designed to profile tRNA fragments from short reads RNA-seq , and tncRNA toolkit (Zahra et al., 2023), which uses tRNAscan-SE (Chan et al., 2021) to index the genome of interest predicting tRNAs sequences. Another option is provided by the unitas software (Gebert et al., 2017), which allows the complete annotation of small RNA sequence datasets, supporting all the species for which non-coding RNA reference sequences are available in the Ensembl databases (Cunningham et al., 2022). ...
